Abstract
Three cDNAs encoding actins were identified in two culturable strains (clades A and F) of the symbiotic dinoflagellates Symbiodinium spp. In a molecular phylogenetic analysis these actin sequences formed a monophyletic group with known dinoflagellate actins, remote from Syact-p that had been isolated from a clade A Symbiodinium strain (HG39). One of the newly identified actin sequences (SyAct-F1) was the most closely related to partial actin cDNA sequences (named AGfact-p and AFcact-p) isolated from adult colonies of two reef corals (Galaxea fascicularis and Favites chinensis) that were inhabited by Symbiodinium spp., suggesting the possibility that the latter two were from the symbionts. Partial AFcact-p sequences could be amplified by PCR using genomic DNA prepared from a symbiotic adult colony of F. chinensis as the template, but not from planula larvae in which zooxanthellae could not be detected, also arguing for the origin of AFcact-p in the symbiont. An expression analysis showed that the levels of the SyAct-A1 mRNA were comparable in symbiotic and non-symbiotic states, and also in motile and non-motile phases in a cultured condition, suggesting its usefulness as a constitutively expressed control gene in expression analysis of Symbiodinium mRNAs.
References
Banaszak AT, LaJeunesse TC, Trench RK (2000) The synthesis of mycosporine-like amino acids (MAAs) by cultured, symbiotic dinoflagellates. J Exp Mar Biol Ecol 249:219–233
Baillie BK, Belda-Baillie CA, Maruyama T (2000) Conspecificity and Indo-Pacific distribution of Symbiodinium genotypes (Dinophyceae) from giant clams. J Phycol 36:1153–1161
Baker AC (2003) Flexibility and specificity in coral—algal symbiosis: diversity, ecology, and biogeography of Symbiodinium. Annu Rev Ecol Evol Syst 34:661–689
Barnes DJ, Chalker BE (1990) Calcification and photosynthesis in reef-building corals and algae. in: Dubinsky Z (ed). Coral reefs:Ecosystems of the world, Vol. 25. Elsevier, Amsterdam, pp. 109–131
Bentis CJ, Kaufman L, Golubic S (2000) Endolithic fungi in reef-building corals (order: Scleractinia) are common, cosmopolitan, and potentially pathogenic. Biol Bull 198:254–260
Carlos AA, Baillie BK, Kawachi M, Maruyama T (1999) Phylogenetic position of Symbiodinium (Dinophyceae) isolates from tridacnids (Bivalvia), cardiids (Bivalva), a sponge (Porifera), a soft coral (Anthozoa), and a free-living strain. J Phycol 35:1054–1062
Fukuda I, Imagawa S, Iwao K, Horiguchi T, Watanabe T (2002) Isolation of actin-encoding cDNAs from symbiotic corals. DNA Res 9:217–223
Fukuda I, Ooki S, Fujita T, Murayama E, Nagasawa H, Isa Y, Watanabe T (2003) Molecular cloning of a cDNA encoding a soluble protein in the coral exoskeleton. Biochem Biophys Res Commun 304:11–17
Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704
Harrison PL, Wallace CC (1990) Reproduction, dispersal and recruitment of scleractinian corals. in: Dubinsky Z (ed). Coral reefs: ecosystems of the world, Vol. 25. Elsevier, Amsterdam, pp 133–207
Hayakawa H, Nakano Y, Andoh T, Watanabe T (2005) Sex-dependent expression of mRNA encoding a major egg protein in the gonochoric coral Galaxea fascicularis. Coral Reefs 24:488–494
Imagawa S, Nakano Y, Watanabe T (2004) Molecular analysis of a major soluble egg protein in the scleractinian coral Favites chinensis. Comp Biochem Physiol 137B:11–19
LaJeunesse TC (2001) Investigating the biodiversity, ecology, and phylogeny of endosymbiotic dinoflagellates of the genus Symbiodinium using the ITS region: in search of a ‘species’ level marker. J Phycol 37:866–880
Muscatine L (1990) The role of symbiotic algae in carbon and energy flux in reef corals. in: Dubinsky Z (ed): Coral reefs:Ecosystems of the world, Vol. 25. Elsevier, Amsterdam, pp. 75–87
Pochon X, LaJeunesse TC, Pawlowski J (2004) Biogeographic partitioning and host specialization among foraminiferan dinoflagellate symbionts (Symbiodinium; Dinophyta). Mar Biol 146:17–27
Rodrìguez F, Oliver JF, Marìn A, Medina JR (1990) The general stochastic model of nucleotide substitutions. J Theor Biol 142:485–501
Rowan R (1998) Diversity and ecology of zooxanthellae on coral reefs. J Phycol 34:407–417
Rowan R, Powers DA (1991) Molecular genetic identification of symbiotic dinoflagellates (zooxanthellae). Mar Ecol Prog Ser 71:65–73
Santos SR, Taylor DJ, Coffroth MA (2001) Genetic comparisons of freshly isolated versus cultured symbiotic dinoflagellates: implications for extrapolating to the intact symbiosis. J Phycol 37:900–912
Schwarz JA, Krupp DA, Weis VM (1999) Late larval development and onset of symbiosis in the scleractinian coral Fungia scutaria. Biol Bull 196:70–79
Takishita K, Ishida K, Maruyama T (2003) An enigmatic GAPDH gene in the symbiotic dinoflagellate genus Symbiodinium and its related species (the order Suessiales): possible lateral gene transfer between two eukaryotic algae, dinoflagellate and euglenophyte. Protist 154:443–454
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Toller WW, Rowan R, Knowlton N (2002) Genetic evidence for a protozoan (phylum Apicomplexa) associated with corals of the Montastraea annularis species complex. Coral Reefs 21:143–146
Trench RK (1987) Dinoflagellates in non-parasitic symbioses. in: Taylor FJR (ed). The biology of dinoflagellates. Blackwell Scientific Publications, Oxford, pp 530–570
van Oppen M (2000) In vitro establishment of symbiosis in Acropora millepora planulae. Coral Reefs 20:200
Veron JEN (2000) Corals of the world. Australian Institute of Marine Science, Townsville, Australia.
Whelan S, Goldman N (2001) A general empirical model of protein evolution derived from multiple protein families using a maximum-likelihood approach. Mol Biol Evol 18:691–699
Yuyama I, Hayakawa H, Endo H, Iwao K, Takeyama H, Maruyama T, Watanabe T (2005) Identification of symbiotically expressed coral mRNAs using a model infection system. Biochem Biophys Res Commun 336:793–798
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Watanabe, T., Kii, Si., Tanaka, J. et al. cDNA cloning and phylogenetic and expression analyses of actin in symbiotic dinoflagellates (Symbiodinium spp.). J Appl Phycol 18, 219–225 (2006). https://doi.org/10.1007/s10811-006-9106-y
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DOI: https://doi.org/10.1007/s10811-006-9106-y