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Highly hydrophilic ordered mesoporous carbon–organic polymer composite and its applications in direct electrochemistry and the possibility of biosensing1

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Abstract

A highly hydrophilic ordered mesoporous carbon–poly(vinyl alcohol) composite with large surface area and good biocompatibility, and exhibiting efficient immobilization and biosensing of protein [e.g., hemoglobin (Hb)] was prepared by intermittent microwave heating techlnique. The as-prepared composite with immobilized Hb is tested for direct electrochemistry and biosensing. The immobilized Hb at the modified electrode had a high adsorption with 32 wt% of the proteins. Moreover, the modified electrode retained its native structure, with improved interfacial electron-communication rate, and showed a good electrochemical performance to detect H2O2, as verified by Raman, UV–Vis absorption, and electrochemical impedance spectroscopies, as well as steady-state current measurements. The proposed biosensor shows a sensitivity of 9.8 µA mM−1 and responds in less than 5 s when the protein concentration ranges from 87.5 to 0.4 µM. This work provides an efficient strategy and a new promising platform for both fundamental investigation of the redox reactions mechanism and the development of third-generation biosensors.

Graphical Abstract

A unique structure and high hydrophilic nature mesoporous carbon has been obtained by a a simple and effective synthesis method. It still retains the ordered mesoporous carbon structure and the mesopore width. The composite enabled a high protein loading and a fast direct electron transfer, resulting in a much higher biocatalytic sensitivity and long-term stability.

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Acknowledgments

The authors acknowledge the support from the National Natural Science Foundation of China (21443004), the 47th (1792) Scientific Research Foundation for the Returned Overseas Chinese Scholars, the Chunhui planning project (Z2014083), the Ministry of Education, and the Technology Research Project of Chongqing Education Board (KJ1401217).

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Authors and Affiliations

  1. Chongqing Key Laboratory of Inorganic Special Functional Materials, Yangtze Normal University, 98 Julong Road, Chongqing, Fuling, China

    Guoqing Zhang, Tuanwu Cao, Huisheng Huang & Peng Zhang

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  1. Guoqing Zhang
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  2. Tuanwu Cao
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  3. Huisheng Huang
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  4. Peng Zhang
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Correspondence to Guoqing Zhang.

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Zhang, G., Cao, T., Huang, H. et al. Highly hydrophilic ordered mesoporous carbon–organic polymer composite and its applications in direct electrochemistry and the possibility of biosensing1. J Appl Electrochem 46, 593–601 (2016). https://doi.org/10.1007/s10800-016-0944-2

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  • DOI: https://doi.org/10.1007/s10800-016-0944-2

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