Abstract
Objective
Due to the high side effects of commonly used drugs and according to the pharmacological properties reported for coumaric acid (CA), this study was designed to determine the impact of CA on acetic acid-induced colitis in rats, considering its possible anti-inflammatory, antioxidant, and anti-apoptotic properties.
Materials and methods
Forty-eight male Wistar rats were divided into 6 equal groups (n = 8). Colitis was induced by acetic acid intrarectally. CA in three different doses (50, 100, and 150 mg/kg) was administrated for 5 days. Finally, the macroscopic and histopathological changes in the colon tissue were examined. The expression of inflammatory and apoptotic genes, including NF-κB, TNF-α, INOS, IL-1β, IL-6, TLR4, Caspase-3, Caspase-8, Bax, Bcl-2 was assessed. In addition, changes in the levels of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), nitrite, and total antioxidant capacity (TAC) were measured in the colon tissue.
Results
Colitis led to a decrease in TAC and the activity levels of CAT and SOD and an increase in the expression of inflammatory and apoptotic genes, MDA, and nitrite levels in the colon. Colitis was also associated with edema and severe damage to the epithelium, infiltration of inflammatory cells, and the presence of ulcers and necrosis in the colon tissue. CA significantly improved the inflammation, oxidative stress, apoptosis, and histopathological indices caused by acetic acid-induced colitis on the colon.
Conclusion
It is concluded that CA probably exerts its positive effects in the management of colitis, through its anti-inflammatory, antioxidant, and anti-apoptotic properties.
Similar content being viewed by others
Data availability
Data regarding the present study are available at the Medical Plants Research Center, Shahrekord University of Medical Sciences.
References
Abdelkader NF, Safar MM, Salem HA (2016) Ursodeoxycholic acid ameliorates apoptotic cascade in the rotenone model of Parkinson’s disease: modulation of mitochondrial perturbations. Mol Neurobiol 53:810–817
Aebi H (1984) [13] Catalase in vitro. Methods Enzymol 105:121–126
Alavala S, Sangaraju R, Nalban N, Sahu BD, Jerald MK, Kilari EK, Sistla R (2019) Stevioside, a diterpenoid glycoside, shows anti-inflammatory property against Dextran Sulphate Sodium-induced ulcerative colitis in mice. Eur J Pharmacol 855:192–201
Andrade AWL, Machado KDC, Machado KDC, Figueiredo DDR, David JM, Islam MT, Uddin SJ, Shilpi JA, Costa JP (2018) In vitro antioxidant properties of the biflavonoid agathisflavone. Chem Cent J 12:1–9
Balmus IM, Ciobica A, Trifan A, Stanciu C (2016) The implications of oxidative stress and antioxidant therapies in inflammatory bowel disease: clinical aspects and animal models. Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association 22:3
Bastaki SM, Amir N, Adeghate E, Ojha S (2021) Nerolidol, a sesquiterpene, attenuates oxidative stress and inflammation in acetic acid-induced colitis in rats. Mol Cell Biochem 476(9):3497–3512. https://doi.org/10.1007/s11010-021-04094-5
Bastaki SM, Al Ahmed MM, Al Zaabi A, Amir N, Adeghate E (2016) Effect of turmeric on colon histology, body weight, ulcer, IL-23, MPO and glutathione in acetic-acid-induced inflammatory bowel disease in rats. BMC Complement Altern Med 16:1–14
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76
Cheng G, Zhao Y, Li H, Wu Y, Li X, Han Q, Dai C, Li Y (2014) Forsythiaside attenuates lipopolysaccharide-induced inflammatory responses in the bursa of Fabricius of chickens by downregulating the NF-κB signaling pathway. Exp Ther Med 7:179–184
Eissa N, Hussein H, Kermarrec L, Ali AY, Marshall A, Metz-Boutigue M-H, Hendy GN, Bernstein CN, Ghia J-E (2018) Chromogranin-A regulates macrophage function and the apoptotic pathway in murine DSS colitis. J Mol Med 96:183–198
Fuentes F, Paredes-Gonzalez X, Kong A-NT (2015) Dietary glucosinolates sulforaphane, phenethyl isothiocyanate, indole-3-carbinol/3, 3′-diindolylmethane: Antioxidative stress/inflammation, Nrf2, epigenetics/epigenomics and in vivo cancer chemopreventive efficacy. Curr Pharmacol Rep 1:179–196
Gilardi D, Fiorino G, Genua M, Allocca M, Danese S (2014) Complementary and alternative medicine in inflammatory bowel diseases: what is the future in the field of herbal medicine? Expert Rev Gastroenterol Hepatol 8:835–846
Gupta RA, Motiwala MN, Mahajan UN, Sabre SG (2018) Protective effect of Sesbania grandiflora on acetic acid induced ulcerative colitis in mice by inhibition of TNF-α and IL-6. J Ethnopharmacol 219:222–232
Haj-Mirzaian A, Amiri S, Amini-Khoei H, Hosseini M-J, Haj-Mirzaian A, Momeny M, Rahimi-Balaei M, Dehpour AR (2017) Anxiety-and depressive-like behaviors are associated with altered hippocampal energy and inflammatory status in a mouse model of Crohn’s disease. Neuroscience 366:124–137
Khan MN, Lane ME, McCarron PA, Tambuwala MM (2018) Caffeic acid phenethyl ester is protective in experimental ulcerative colitis via reduction in levels of pro-inflammatory mediators and enhancement of epithelial barrier function. Inflammopharmacology 26:561–569
Khan M, Liu H, Wang J, Sun B (2020) Inhibitory effect of phenolic compounds and plant extracts on the formation of advance glycation end products: a comprehensive review. Food Res Int 130:108933
Kim Y, Wu AG, Jaja-Chimedza A, Graf BL, Waterman C, Verzi MP, Raskin I (2017) Isothiocyanate-enriched moringa seed extract alleviates ulcerative colitis symptoms in mice. PLoS ONE 12:e0184709
Lee W, Yang S, Lee C, Park EK, Kim K-M, Ku S-K, Bae J-S (2019) Aloin reduces inflammatory gene iNOS via inhibition activity and p-STAT-1 and NF-κB. Food Chem Toxicol 126:67–71
Liu X, Wang JM (2011) Iridoid glycosides fraction of Folium syringae leaves modulates NF-κ B signal pathway and intestinal epithelial cells apoptosis in experimental colitis. PLoS ONE 6:e24740
Liu D-M, Zhou C-Y, Meng X-L, Wang P, Li W (2018) Thymol exerts anti-inflammatory effect in dextran sulfate sodium-induced experimental murine colitis. Trop J Pharm Res 17:1803–1810
Lou Z, Wang H, Rao S, Sun J, Ma C, Li J (2012) p-Coumaric acid kills bacteria through dual damage mechanisms. Food Control 25:550–554
Macpherson B, Pfeiffer C (1978) Experimental production of diffuse colitis in rats. Digestion 17:135–150
Magrone T, Magrone M, Russo MA, Jirillo E (2020) Recent advances on the anti-inflammatory and antioxidant properties of red grape polyphenols: In vitro and in vivo studies. Antioxidants 9:35
Mahmoud TN, El-Maadawy WH, Kandil ZA, Khalil H, El-Fiky NM, el Alfy TSM (2021) Canna x generalis LH Bailey rhizome extract ameliorates dextran sulfate sodium-induced colitis via modulating intestinal mucosal dysfunction, oxidative stress, inflammation, and TLR4/NF-ҡB and NLRP3 inflammasome pathways. J Ethnopharmacol 269:113670
Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175
Murad HA, Abdallah HM, Ali SS (2016) Mentha longifolia protects against acetic-acid induced colitis in rats. J Ethnopharmacol 190:354–361
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358
Omidi-Ardali H, Lorigooini Z, Soltani A, Balali-Dehkordi S, Amini-Khoei H (2019) Inflammatory responses bridge comorbid cardiac disorder in experimental model of IBD induced by DSS: protective effect of the trigonelline. Inflammopharmacology 27:1265–1273
Peng J, Zheng T-T, Liang Y, Duan L-F, Zhang Y-D, Wang L-J, He G-M, Xiao H-T (2018) p-Coumaric acid protects human lens epithelial cells against oxidative stress-induced apoptosis by MAPK signaling, Oxidative Medicine and Cellular Longevity, vol 2018. p 8549052. https://doi.org/10.1155/2018/8549052
Pourkhodadad S, Alirezaei M, Moghaddasi M, Ahmadvand H, Karami M, Delfan B, Khanipour Z (2016) Neuroprotective effects of oleuropein against cognitive dysfunction induced by colchicine in hippocampal CA1 area in rats. J Physiol Sci 66:397–405
Prince PSM, Roy AJ (2013) p-Coumaric acid attenuates apoptosis in isoproterenol-induced myocardial infarcted rats by inhibiting oxidative stress. Int J Cardiol 168:3259–3266
Rafiee Z, Moaiedi MZ, Gorji AV, Mansouri E (2020) p-Coumaric acid mitigates doxorubicin-induced nephrotoxicity through suppression of oxidative stress, inflammation and apoptosis. Arch Med Res 51:32–40
Ramachandran A, Madesh M, Balasubramanian KA (2000) Apoptosis in the intestinal epithelium: its relevance in normal and pathophysiological conditions. J Gastroenterol Hepatol 15:109–120
Ravindran R, Swamy MK, Jaganathan R (2019) Therapeutic potential of plant polyphenolics and their mechanistic action against various diseases. Springer, Natural Bio-Active Compounds
Rees V (1998) Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines. Clin Exp Immunol 114:385–391
Robbins SL, Cotran RS (1979) Pathologic basis of disease. Saunders
Sales-Campos H, Basso P, Alves V, Fonseca M, Bonfá G, Nardini V, Cardoso C (2015) Classical and recent advances in the treatment of inflammatory bowel diseases. Braz J Med Biol Res 48:96–107
Scorrano L, Korsmeyer SJ (2003) Mechanisms of cytochrome c release by proapoptotic BCL-2 family members. Biochem Biophys Res Commun 304:437–444
Shaker ME, Ashamallah SA, Houssen ME (2014) Celastrol ameliorates murine colitis via modulating oxidative stress, inflammatory cytokines and intestinal homeostasis. Chem Biol Interact 210:26–33
Sharma SH, Rajamanickam V, Nagarajan S (2018) Antiproliferative effect of p-Coumaric acid targets UPR activation by downregulating Grp78 in colon cancer. Chem Biol Interact 291:16–28
Shen Y, Song X, Li L, Sun J, Jaiswal Y, Huang J, Liu C, Yang W, Williams L, Zhang H (2019) Protective effects of p-coumaric acid against oxidant and hyperlipidemia-an in vitro and in vivo evaluation. Biomed Pharmacother 111:579–587
Tekeli İO, Ateşşahin A, Sakin F, Aslan A, ÇERIBAŞı, S. & YIPEL, M. (2019) Protective effects of conventional and colon-targeted lycopene and linalool on ulcerative colitis induced by acetic acid in rats. Inflammopharmacology 27:313–322
Tian Q, Bravo Iniguez A, Sun Q, Wang H, Du M, Zhu MJ (2021) Dietary Alpha-Ketoglutarate promotes epithelial metabolic transition and protects against DSS-induced colitis. Mol Nutr Food Res 65:2000936
Triantafillidis JK, Triantafyllidi A, Vagianos C, Papalois A (2016) Favorable results from the use of herbal and plant products in inflammatory bowel disease: evidence from experimental animal studies. Ann Gastroenterol Q Publ Hell Soc Gastroenterol 29:268
Wallace JL, Keenan CM (1990) An orally active inhibitor of leukotriene synthesis accelerates healing in a rat model of colitis. Am J Physiol Gastrointest Liver Physiol 258:G527–G534
Wallace KL, Zheng L-B, Kanazawa Y, Shih DQ (2014) Immunopathology of inflammatory bowel disease. World J Gastroenterol: WJG 20:6
Wang L, Liu H, Zhang L, Wang G, Zhang M, Yu Y (2017) Neuroprotection of dexmedetomidine against cerebral ischemia-reperfusion injury in rats: involved in inhibition of NF-κB and inflammation response. Biomol Ther 25:383
Zhu H, Li YR (2012) Oxidative stress and redox signaling mechanisms of inflammatory bowel disease: updated experimental and clinical evidence. Exp Biol Med 237:474–480
Acknowledgements
The authors thank the Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran, for supporting this study.
Funding
This study was supported by a research grant (5186) from Shahrekord University of Medical Sciences, Shahrekord, Iran.
Author information
Authors and Affiliations
Contributions
All the authors were involved in the design, Material preparation, data collection and analysis, manuscript writing, and editing. All the authors read and approved the final draft of the manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors have no conflicts of interest to declare regarding the study described in this article and the preparation of the manuscript.
Ethical approval
All procedures were carried out under the regulations of the University and the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (ethical code: IR.SKUMS.REC.1399.113) and Guide for the Care and Use of Laboratory Animals (8th edition, National Academies Press).
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Ghasemi-Dehnoo, M., Amini-Khoei, H., Lorigooini, Z. et al. Coumaric acid ameliorates experimental colitis in rats through attenuation of oxidative stress, inflammatory response and apoptosis. Inflammopharmacol 30, 2359–2371 (2022). https://doi.org/10.1007/s10787-022-01074-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10787-022-01074-z