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Effect of aqueous extract of seed of broccoli on inflammatory cytokines and Helicobacter pylori infection: a randomized, double-blind, controlled trial in patients without atrophic gastritis

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A Correction to this article was published on 01 September 2022

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Abstract

The purpose of this study was to investigate the anti-inflammatory effect of an aqueous extract of seed of broccoli (AESB) in Helicobacter pylori (HP)-infected patients without atrophic gastritis. This was a double-centre, randomized, double-blind, controlled study. A total of 110 HP-infected subjects were randomized to receive either AESB or placebo for 2 months. Inflammatory cytokine (IL-8, IFN-γ, TNF-α, CRP, IL-17A, IL-1β, IL-18), pepsinogen I, II (PG I, PG II), and gastrin-17 (G-17) measurements and 13C-urea breath tests were performed at baseline and at 60 days. At 60 days, there was no significant difference in any of the inflammatory cytokines, pepsinogen or gastrin between the two groups. However, IL-8, IFN-γ, PG I, PG I/PG II ratio (PGR), and G-17 were reduced by 9.02 pg/mL, 5.08 pg/mL, 24.56 ng/mL, 1.75 and 0.3 pmol/L, respectively, in the AESB group compared with baseline (all P < 0.05). The HP eradication rates in the AESB group and placebo group were 11.11 and 3.70% at 60 days, respectively (P > 0.05). No treatment-related adverse events were reported. Thus, AESB may reduce the risk of gastric mucosal lesions and decrease the risk of gastric cancer by relieving inflammatory cytokines. The safety profile of AESB was satisfactory. This study is registered with the Chinese Clinical Trials Registry (Registration No. ChiCTR2100054249).

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Data availability

Data is available upon reasonable request from the corresponding authors.

Change history

Abbreviations

HP:

Helicobacter pylori

AESB:

Aqueous extract of seed of broccoli

IFN-γ:

Interferon-γ

TNF-α:

Tumour necrosis factor-α

CRP:

C-reactive protein

IL-17A:

Interleukin-17 A

IL-1β:

Interleukin-1β

IL-8:

Interleukin-8

IL-18:

Interleukin-18

PG I:

Pepsinogen I

PG II:

Pepsinogen II

G-17:

Gastrin-17

ADRs:

Adverse drug reactions

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Funding

This research was funded by BYHEALTH, grant number TY0191115, the Fundamental Research Funds for the Central Universities (No. lzujbky-2019–4), Gansu Province Science Foundation for Youths (No.21JR7RA507) with Lipeng Jing as the funding receiver. and Cuiying Science and Technology Innovation Project of Lanzhou University Second Hospital (CY-2019-QN19) with Lei Wang as the funding receiver. The intervention formulas were donated by BYHEALTH.

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Author notes

  1. Kai Guo, Lei Wang, Jinli Mahe authors contributed equally.

Authors and Affiliations

  1. Institute of Epidemiology and Statistics, School of Public Health, Lanzhou University, Lanzhou, Gansu Province, China

    Kai Guo, Jinli Mahe, Xiaoming Liu, Xuejiao Zeng, Xiaobin Hu & Lipeng Jing

  2. Department of Neurology, Lanzhou University Second Hospital, Lanzhou, Gansu Province, China

    Lei Wang

  3. Jing Yuan County Hospital of Traditional Chinese Medicine, Baiyin, Gansu Province, China

    Liansheng Li

  4. Bai Yin Third People’s Hospital, Baiyin, Gansu Province, China

    Shaojiang Jiao & Haiyan Wang

  5. Institute of Nutrition and Health, BYHEALTH, Guangzhou, Guangdong Province, China

    Yanru Xie

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  1. Kai Guo
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Contributions

LJ and LW: conceived and designed the research; KG, LW, JM, XL, XZ: collected the data; KG: performed the statistical analysis; KG and LJ wrote the manuscript; YX revised the manuscript; XH, LL, SJ and HW contributed to the discussion; All authors read, revised and approved the final manuscript. LJ: acts as guarantor for this study and publication.

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Correspondence to Xiaobin Hu or Lipeng Jing.

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Conflict of interest

Yanru Xie is employed by BYHEALTH. However, this author played no role in collecting the data, analyzing the data or interpreting the results. All other authors have no relevant financial or non-financial interests to disclose.

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This study was conducted in accordance with the Declaration of Helsinki, and approved by the Ethics Committee of the School of Public Health of Lanzhou University (Approval No. IRB19122001).

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Informed consent was obtained from all individual participants included in the study. Details that might disclose the identity of the subjects under study have been omitted.

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Guo, K., Wang, L., Mahe, J. et al. Effect of aqueous extract of seed of broccoli on inflammatory cytokines and Helicobacter pylori infection: a randomized, double-blind, controlled trial in patients without atrophic gastritis. Inflammopharmacol 30, 1659–1668 (2022). https://doi.org/10.1007/s10787-022-01030-x

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