Skip to main content

Advertisement

SpringerLink
Log in

Protective Effects of Arachidonic Acid Against Paraquat-Induced Pulmonary Injury

  • Published:
Inflammation Aims and scope Submit manuscript

ABSTRACT

In this study, we aimed to study the effects of arachidonic acid (AA) on acute lung injury (ALI) caused by paraquat (PQ) in mice. Male Kunming mice were randomly divided into three groups: control group, PQ group, and PQ + AA group (n = 24). The mice in the PQ and PQ + AA groups received a single oral dose of 20 mg/kg bodyweight PQ, and the mice of the PQ + AA group were challenged by 500 mg/kg bodyweight AA posttreatment 2 h after PQ administration. The results indicated that the administration of AA significantly increased the activity of superoxide dismutase (SOD), decreased the activity of myeloperoxidase (MPO), the content of malondialdehyde (MDA), and the level of lactate dehydrogenase (LDH). Pathological examination also revealed that AA effectively alleviated PQ-induced histological damage. Furthermore, AA significantly reduced PQ-induced upregulations of inflammatory mediators such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-8. These results demonstrated that AA had effective protection against PQ-induced ALI.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

REFERENCES

  1. Chen, J.J., T. Zeng, Y. Bi, et al. 2011. Docosahexaenoic acid (DHA) attenuated paraquat induced lung damage in mice. Inhalation Toxicology 25: 9–16.

    Article  CAS  Google Scholar 

  2. Seok, S.J., H.W. Gil, D.S. Jeong, et al. 2009. Paraquat intoxication in subjects who attempt suicide: why they chose paraquat. Korean Journal of Internal Medicine 24: 247–251.

    Article  PubMed Central  PubMed  Google Scholar 

  3. Zerin, T., Y.S. Kim, S.Y. Hong, et al. 2012. Protective effect of methylprednisolone on paraquat-induced A549 cell cytotoxicity via induction of efflux transporter, P-glycoprotein expression. Toxicology Letters 208: 101–107.

    Article  CAS  PubMed  Google Scholar 

  4. Tomita, M., T. Okuyama, H. Katsuyama, et al. 2007. Mouse model of paraquat-poisoned lungs and its gene expression profile. Toxicology 231: 200–209.

    Article  CAS  PubMed  Google Scholar 

  5. Awadalla, E.A. 2012. Efficacy of vitamin C against liver and kidney damage induced by paraquat toxicity. Experimental and Toxicologic Pathology 64: 431–434.

    Article  CAS  PubMed  Google Scholar 

  6. Uzun, F.G., F. Demir, S. Kalender, et al. 2010. Protective effect of catechin and quercetin on chlorpyrifos-induced lung toxicity in male rats. Food and Chemical Toxicology 48: 1714–1720.

    Article  CAS  PubMed  Google Scholar 

  7. Grosicka-Maciag, E., D. Kurpios-Piec, M. Szumilo, et al. 2011. Protective effect of N-acetyl-L-cysteine against maneb induced oxidative and apoptotic injury in Chinese hamster V79 cells. Food and Chemical Toxicology 49: 1020–1025.

    Article  CAS  PubMed  Google Scholar 

  8. Dinis-Oliveira, R.J., J.A. Duarte, A. Sánchez-Navarro, et al. 2008. Paraquat poisonings: mechanisms of lung toxicity, clinical features, and treatment. Critical Reviews in Toxicology 38: 13–71.

    Article  CAS  PubMed  Google Scholar 

  9. Valko, M., D. Leibfritz, J. Moncol, et al. 2007. Free radicals and antioxidants in normal physiological functions and human disease. International Journal of Biochemistry & Cell Biology 39: 44–84.

    Article  CAS  Google Scholar 

  10. Riahi, B., H. Rafatpanah, M. Mahmoudi, et al. 2010. Immunotoxicity of paraquat after subacute exposure to mice. Food and Chemical Toxicology 48: 1627–1631.

    Article  CAS  PubMed  Google Scholar 

  11. Rahman, I., and W. MacNee. 2000. Regulation of redox glutathione levels and gene transcription in lung inflammation: therapeutic approaches. Free Radical Biology and Medicine 28: 1405–1420.

    Article  CAS  PubMed  Google Scholar 

  12. Kim, S., L. Dayani, P.A. Rosenberg, et al. 2010. RIP1 kinase mediates arachidonic acid-induced oxidative death of oligodendrocyte precursors. International Journal of Psychophysiology 2: 137–147.

    CAS  Google Scholar 

  13. Wang, Z.J., C.L. Liang, G.M. Li, et al. 2006. Neuroprotective effects of arachidonic acid against oxidative stress on rat hippoeampal slices. Chemico-Biological Interactions 163: 207–217.

    Article  CAS  PubMed  Google Scholar 

  14. Chen, Y., Y.C. Nie, Y.L. Luo, et al. 2013. Protective effects of naringin against paraquat-induced acute lung injury and pulmonary fibrosis in mice. Food and Chemical Toxicology 58: 133–140.

    Article  CAS  PubMed  Google Scholar 

  15. Sun, S., H. Wang, G. Zhao, et al. 2011. Complement inhibition alleviates paraquat-induced acute lung injury. American Journal of Respiratory Cell and Molecular Biology 45: 834–842.

    Article  CAS  PubMed  Google Scholar 

  16. Park, H.K., S.J. Kim, Y. Kwon do, et al. 2010. Protective effect of quercetin against paraquat-induced lung injury in rats. Life Science 87: 181–186.

    Article  CAS  Google Scholar 

  17. Lee, J., W. Kwon, Y. Jo, et al. 2008. Protective effects of ethyl pyruvate treatment on paraquat-intoxicated rats. Human & Experimental Toxicology 27: 49–54.

    Article  CAS  Google Scholar 

  18. Podder, B., Y.S. Kim, T. Zerin, et al. 2012. Antioxidant effect of silymarin on paraquat-induced human lung adenocarcinoma A549 cell line. Food and Chemical Toxicolog 50: 3206–3214.

    Article  CAS  Google Scholar 

  19. Cristóvão, A.C., D.H. Choi, G. Baltazar, et al. 2009. The role of NADPH oxidase 1-derived reactive oxygen species in paraquat-mediated dopaminergic cell death. Antioxidants & Redox Signaling 11: 2105–2118.

    Article  Google Scholar 

  20. Cui, Y., J. Robertson, S. Maharaj, et al. 2011. Oxidative stress contributes to the induction and persistence of TGF-beta1 induced pulmonary fibrosis. International Journal of Biochemistry & Cell Biology 43: 1122–1133.

    Article  CAS  Google Scholar 

  21. Reumaux, D., M. de Boer, A.B... Meijer, et al. 2003. Expression of myeloperoxidase (MPO) by neutrophils is necessary for their activation by anti-neutrophil cytoplasm autoantibodies (ANCA) against MPO. Journal of Leukocyte Biology 73: 841–849.

Download references

Author information

Authors and Affiliations

  1. Pulmonary Department, Affiliated Hospital of QingDao University, Qingdao, 266003, People’s Republic China

    Jianxin Du

  2. Respiratory Department of Internal Medicine, People’s Hospital of Rizhao, Rizhao, 267807, People’s Republic China

    Xia Li

  3. Pulmonary Department, Affiliated Hospital of Qingdao University, Qingdao, 266003, People’s Republic China

    CunZhi Lin

  4. Respiratory Department, People‘s Hospital of Rizhao Lanshan, Lanshanxi Road, Rizhao, 267807, People‘s Republic China

    Xigang He

Authors
  1. Jianxin Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xia Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. CunZhi Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xigang He
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xigang He.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Du, J., Li, X., Lin, C. et al. Protective Effects of Arachidonic Acid Against Paraquat-Induced Pulmonary Injury. Inflammation 38, 1458–1463 (2015). https://doi.org/10.1007/s10753-015-0120-6

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10753-015-0120-6

KEY WORDS

Search

Navigation