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Follistatin-like protein 1 suppressed pro-inflammatory cytokines expression during neuroinflammation induced by lipopolysaccharide

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Abstract

Follistain-like protein 1 (FSTL1), has been recently demonstrated to be involved in the embryo development of nervous system and glioblastoma. However, the role of FSTL1 in neuroinflammation remains unexplored. In this study, the expression of FSTL1 in astrocytes was verified and its role was studied in neuroinflammation induced by in vivo intracerebroventricular (ICV) injection of lipopolysaccharide (LPS) or LPS treatment to astrocytes in vitro. FSTL1 was significantly induced after ICV LPS injection or LPS treatment. FSTL1 suppressed upregulation of pro-inflammatory cytokines in astrocytes after LPS treatment. Moreover, FSTL1 downregulated expression of pro-inflammatory cytokines through suppressing MAPK/p-ERK1/2 pathway in astrocytes. Our results suggest that FSTL1 may play an anti-inflammatory role in neuroinflammation mediated by astrocytes.

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Abbreviations

FSTL1:

Follistatin-like protein 1

hrFSTL1:

Human recombined Follistatin-like protein 1

LPS:

Lipopolysaccharide

ICV:

Intracerebroventricular

N.S.:

Normal saline

CNS:

Central nervous system

HIF:

Hypoxia induced factor

TNF-α:

Tumor necrosis factor alpha

IL-1β:

Interleukin 1 beta

IL-6:

Interleukin 6

COX2:

Cyclooxygenase-2

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Acknowledgements

We thank Chong-Bo Yang for his patient review and critical thinking of our manuscript. This work was supported by the General Program of National Natural Science Foundation of China (Grant No. 81572191).

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    Authors and Affiliations

    1. Department of Orthopaedics, Qilu Hospital of Shandong University, Jinan, 250012, China

      Kai-Yuan Cheng, Yi Liu, Ying-Guang Han, Jing-Kun Li, Jia-Lin Jia, Bin Chen, Zhi-Xiao Yao, Lin Nie & Lei Cheng

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    1. Kai-Yuan Cheng
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    2. Yi Liu
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    Correspondence to Lei Cheng.

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    Kai-Yuan Cheng and Yi Liu have contributed equally to this work.

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    Cheng, KY., Liu, Y., Han, YG. et al. Follistatin-like protein 1 suppressed pro-inflammatory cytokines expression during neuroinflammation induced by lipopolysaccharide. J Mol Hist 48, 63–72 (2017). https://doi.org/10.1007/s10735-016-9706-z

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    • DOI: https://doi.org/10.1007/s10735-016-9706-z

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