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Genome-wide identification and response to exogenous hormones and pathogens of the TIFY gene family in Fragaria ananassa

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Abstract

TIFY protein is a plant-specific transcription factor widely found in land plants and plays an important role in plant growth and development, signal transduction and stress response. Although many TIFY gene families have been identified and studied in plants, they have not been well described in octaploid strawberry. In this study, 54 TIFY family genes in strawberry were identified and their biological information was analyzed. The phylogenetic tree showed that FaTIFY genes were divided into four subfamilies: TIFY, JAZ, ZML and PPD. The JAZ subfamily was the largest and can be further divided into five groups. These genes are located on six chromosomes and exhibit a motif range of 3 to 8. Promoter cis-element analysis showed that most FaTIFY genes contain cis-elements associated with plant hormones responses. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that FaTIFY genes had different expression patterns under methyl jasmonate (MeJA), and salicylic acid (SA) treatments. At the same time, we also examined the expression pattern of TIFY gene after boea mold infection and predicted the binding of TIFY protein to WRKY transcription factor, providing a theoretical basis for exploring the role of TIFY gene in hormone delivery and plant defense in strawberry.

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Funding

This research was supported by the National Natural Science Foundation of China (32272370).

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Authors and Affiliations

  1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food Science and Engineering, Ningbo University, Ningbo, 315800, China

    Siyao Tong, Yi Chen, Yingying Wei, Shu Jiang, Jianfen Ye, Feng Xu & Xingfeng Shao

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  1. Siyao Tong
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  2. Yi Chen
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Contributions

Xingfeng Shao (XS), Yi Chen (YC), and Siyao Tong (ST) conceptualized and designed the experiments. ST conducted the experiments and drafted the manuscript. Data analysis was performed by ST, YC, and Yingying Wei (YW). The manuscript’s initial draft was critically reviewed and edited by XS, YC, YW, Jianfen Ye (JY), Shu Jiang (SJ), and Feng Xu (FX). Funding acquisition was managed by XS.

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Tong, S., Chen, Y., Wei, Y. et al. Genome-wide identification and response to exogenous hormones and pathogens of the TIFY gene family in Fragaria ananassa. Plant Growth Regul (2024). https://doi.org/10.1007/s10725-024-01147-9

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