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Regulation of auxin accumulation and perception at different developmental stages in carrot

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Abstract

Auxins are a class of small indolic compounds that affect plant growth and developmental processes. However, the molecular mechanisms underlying auxin accumulation and perception during carrot growth and development are still unclear. To address this, carrot samples from five stages were collected. Morphological characteristics and auxin accumulation were also investigated during carrot development. IAA levels underwent great changes at five successive stages, and IAA contents in the petioles and leaves were relatively higher than those in the roots. A total of 18 genes related to auxin biosynthesis and signaling were identified and their expression profiles were determined using quantitative real-time PCR. Transcript levels of most genes were not well correlated with auxin accumulation, indicating that IAA biosynthesis pathway may be regulated through a complex network. The results indicated that auxin may regulate carrot growth and development in tissue-specific and stage-dependent manner. This study would provide valuable resources for genetic and molecular research focused on carrot growth and development.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

ARF:

Auxin response factor

Aux/IAA:

Auxin/indole-3-acetic acid

GH3:

Gretchen Hagen3

IAA:

Indole-3-acetic acid

IAAld:

Indole-3-acetaldehyde

IAM:

Indole-3-acetamide

IAN:

Indole-3-acetonitrile

IAOx:

Indole-3-acetaldoxime

IPyA:

Indole-3-pyruvic acid

NAA:

1-Naphthaleneacetic acid

qRT-PCR:

Quantitative real-time PCR

TAA:

Tryptophan aminotransferase

TIR1:

Transport inhibitor response

Trp:

Tryptophan

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Acknowledgments

The research was supported by the New Century Excellent Talents in University (NCET-11-0670); Jiangsu Natural Science Foundation (BK20130027); Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Author contributions

Conceived and designed the experiments: A.S.X., X.J.W. Performed the experiments: X.J.W., G.L.W., X.S., Z.S.X., F.W., A.S.X. Analyzed the data: X.J.W. Contributed reagents/materials/analysis tools: A.S.X. Wrote the paper: X.J.W. Revised the paper: A.S.X., X.J.W. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Xue-Jun Wu, Guang-Long Wang, Xiong Song, Zhi-Sheng Xu, Feng Wang & Ai-Sheng Xiong

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  1. Xue-Jun Wu
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  2. Guang-Long Wang
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  3. Xiong Song
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  4. Zhi-Sheng Xu
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Correspondence to Ai-Sheng Xiong.

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Wu, XJ., Wang, GL., Song, X. et al. Regulation of auxin accumulation and perception at different developmental stages in carrot. Plant Growth Regul 80, 243–251 (2016). https://doi.org/10.1007/s10725-016-0161-3

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