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Expression of rice gene OsMSR4 confers decreased ABA sensitivity and improved drought tolerance in Arabidopsis thaliana

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Abstract

The small heat shock proteins (sHSPs) are most prevalent in plants and are believed to play an important role in stress tolerance. Our microarray and qRT-PCR analyses of rice plants showed that the gene Oryza sativa Multi-Stress-Responsive 4 (OsMSR-4) is induced by heat, drought, and cold in different tissues at various developmental stages. OsMSR-4 encodes a Class III sHSP. Its expression in Arabidopsis thaliana conferred enhanced tolerance to drought accompanied by altered expression of other stress-related genes. Under drought conditions, levels of free proline were higher in transgenic plants than in the wild-type. The transgenics also showed decreased sensitivity to abscisic acid (ABA) during the seed germination and post-germination stages. Our study provides evidence that OsMSR4 has a key role in regulating plant responses to ABA and drought.

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Abbreviations

ABA:

Abscisic acid

ACD:

“a-crystallin” domain

MS:

Murashige and Skoog

NLS:

Nuclear localization signal

ORF:

Open reading frame

OsMSR-4 :

Oryza sativa Multi-Stress-Responsive Gene 4

qRT-PCR:

Quantitative real-time PCR

RT-PCR:

Reverse-transcription PCR

sHSPs:

Small heat shock proteins

WT:

Wild type

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Acknowledgments

This research was supported by the Science and Technology Key Project of Hu Nan Province, P. R. China (S2011F2022).

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Authors and Affiliations

  1. Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, People’s Republic of China

    Xuming Yin, Lifang Huang, Manling Wang, Guoyun Xu & Xinjie Xia

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Xin Zhang

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  1. Xuming Yin
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  2. Lifang Huang
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  3. Xin Zhang
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  4. Manling Wang
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  5. Guoyun Xu
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  6. Xinjie Xia
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Correspondence to Xinjie Xia.

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Yin, X., Huang, L., Zhang, X. et al. Expression of rice gene OsMSR4 confers decreased ABA sensitivity and improved drought tolerance in Arabidopsis thaliana . Plant Growth Regul 75, 549–556 (2015). https://doi.org/10.1007/s10725-014-0020-z

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  • DOI: https://doi.org/10.1007/s10725-014-0020-z

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