Abstract
The effect of excess cadmium (Cd: 0.0, 0.25, 0.5, 1.0 and 2.0 mM) on growth and ascorbate oxidase (AO) activity was investigated in barley (Hordeum vulgare L. cv. Jubilant) roots. The study employed a filter-paper technique to germinate and grow the germinating seeds following imbibition with respective Cd treatments for 4 h at 25 °C in darkness. Cd was required at 1.0 mM to affect 50% root growth inhibition 72 h after the treatment. This Cd-induced root growth inhibition was accompanied by a corresponding loss of plasma membrane integrity in root cells as evaluated by Evans blue uptake. Excess Cd (1.0 and 2.0 mM) significantly inhibited the AO activity in all the analysed fractions of barley roots such as extracellular, soluble, cell wall (CW)- and membrane-bound fractions. AO was localized in the apoplast, and its highest specific activity was detected in the CW II fraction obtained by extraction with 1.0 M NaCl from purified cell walls. The analysis of AO isozyme profile showed that besides the reduced activity of two anionic and two cationic isozymes, one cationic AO isozyme was activated during excess Cd treatment, which could be detected in cell wall fractions CW II, III and IV.
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Abbreviations
- AO:
-
ascorbate oxidase
- Cd:
-
cadmium
- CW:
-
cell wall
- ROS:
-
reactive oxygen species
References
O. Arrigoni L. Gara ParticleDe F. Tommasi R. Liso (1992) ArticleTitleChanges in the ascorbate system during seed development of Vicia faba L. Plant Physiol. 99 235–238 Occurrence Handle1:CAS:528:DyaK38Xkt1egurc%3D Occurrence Handle16668855
O. Arrigoni E. Chinni S. Ciraci M.C. Tullio ParticleDe (2003) ArticleTitle In vivo elicitation of ascorbate oxidase activity by dioxygen and its possible role in photosynthesizing leaves Rend. Fis. Acc. Lincei. 14 127–134
S.V. Avery (2001) ArticleTitleMetal toxicity in yeasts and the role of oxidative stress Adv. Appl. Microbiol. 49 111–142 Occurrence Handle1:CAS:528:DC%2BD3sXjsl2nsLc%3D Occurrence Handle11757348
C.J. Baker N.M. Mock (1994) ArticleTitleAn improved method for monitoring cell death in cell suspension and leaf disc assays using Evans blue Plant Cell Tiss. Org. Cult. 39 7–12 Occurrence Handle10.1007/BF00037595
M.N. Bradford (1976) ArticleTitleA rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein–dye binding Anal. Biochem. 72 248–254 Occurrence Handle10.1016/0003-2697(76)90527-3 Occurrence Handle1:CAS:528:DyaE28XksVehtrY%3D Occurrence Handle942051
J.M. Cutler D.W. Rians (1974) ArticleTitleCharacterisation of cadmium uptake by plant tissue Plant Physiol. 54 67–71 Occurrence Handle1:CAS:528:DyaE2cXls1KnsLk%3D Occurrence Handle16658840
A. Chaoui A. Mazhoudi M.H. Ghorbal E. El Ferjani (1997) ArticleTitleCadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.) Plant Sci. 127 139–147 Occurrence Handle10.1016/S0168-9452(97)00115-5 Occurrence Handle1:CAS:528:DyaK2sXltl2ksLw%3D
A. Chaoui B. Jarrar E. El Ferjani (2004) ArticleTitleEffects of cadmium and copper on peroxidaseNADH oxidase and IAA oxidase activities in cell wall, soluble and microsomal membrane fractions of pea roots J. Plant Physiol. 161 1225–1234 Occurrence Handle10.1016/j.jplph.2004.02.002 Occurrence Handle1:CAS:528:DC%2BD2MXhvFeguw%3D%3D Occurrence Handle15602814
U. Cho N. Seo (2005) ArticleTitleOxidative stress in Arabidopsis thaliana exposed to cadmium is due to hydrogen peroxide accumulation Plant Sci. 168 113–120 Occurrence Handle10.1016/j.plantsci.2004.07.021 Occurrence Handle1:CAS:528:DC%2BD2cXpsFSltb4%3D
M.C. Tullio ParticleDe R. Liso O. Arrigoni (2004) ArticleTitleAscorbic acid oxidase: an enzyme in search of a role Biol. Plant. 48 161–166
M. Esaka J. Imagi K. Suzuki K. Kubota (1988) ArticleTitleFormation of ascorbate oxidase in cultured pumpkin cells Plant Cell Physiol. 29 231–235 Occurrence Handle1:CAS:528:DyaL1cXitVWrs7g%3D
M. Esaka K. Fujisawa M. Goto Y. Kisu (1992) ArticleTitleRegulation of ascorbate oxidase expression in pumpkin by auxin and copper Plant Physiol. 100 231–237 Occurrence Handle1:CAS:528:DyaK38XmtlShsr0%3D Occurrence Handle16652952
E. Fodor A. Szabó-Nagy L. Erdei (1995) ArticleTitleThe effects of cadmium on the fluidity and H+-ATPase activity of plasma membrane from sunflower and wheat roots J. Plant Physiol. 147 87–92 Occurrence Handle1:CAS:528:DyaK2MXpsVKjurg%3D
M. Fojtová A. Kovařík (2000) ArticleTitleGenotoxic effect of cadmium is associated with apoptotic changes in tobacco cells Plant Cell Environ. 23 531–537
J.J. Hart R.M. Welch W.A. Norwell L.V. Kochian (2002) ArticleTitleTransport interactions between cadmium and zinc in roots of bread and durum wheat seedlings Physiol. Plant. 116 73–78 Occurrence Handle10.1034/j.1399-3054.2002.1160109.x Occurrence Handle1:CAS:528:DC%2BD38Xnt1els70%3D Occurrence Handle12207664
M. Inouhe S. Ninomiya H. Tohoyama M. Joho T. Murayama (1994) ArticleTitleDifferent characteristics of roots in the cadmium-tolerance and Cd-binding formation between mono- and dicotyledonous plants J. Plant Res. 107 201–207 Occurrence Handle1:CAS:528:DyaK2MXhvFehsb8%3D
K. Jiang L.J. Feldman (2003) ArticleTitleRoot meristem establishment and maintenance: the role of auxin J. Plant Growth Regul. 21 432–440 Occurrence Handle10.1007/s00344-002-0037-9 Occurrence Handle1:CAS:528:DC%2BD3sXlslWntLs%3D
N. Kato M. Esaka (2000) ArticleTitleExpansion of transgenic tobacco protoplasts expressing pumpkin ascorbate oxidase is more rapid than that of wild-type protoplasts Planta 210 1018–1022 Occurrence Handle10.1007/s004250050712 Occurrence Handle1:CAS:528:DC%2BD3cXjtVWrtLY%3D Occurrence Handle10872237
U.K. Laemmli (1970) ArticleTitleCleavage of structural proteins during the assembly of the head of the bacteriophage T4 Nature 227 680–685 Occurrence Handle10.1038/227680a0 Occurrence Handle1:CAS:528:DC%2BD3MXlsFags7s%3D Occurrence Handle5432063
L. Lin J.E. Varner (1991) ArticleTitleExpression of ascorbate acid oxidase in zucchini squash (Cucurbita pepo L.) Plant Physiol. 96 159–165 Occurrence Handle1:CAS:528:DyaK3MXkt1Kju74%3D Occurrence Handle16668145 Occurrence Handle10.1104/pp.96.1.159
R. Liso M.C. Tullio ParticleDe S. Ciraci R. Balestrini N. Rocca ParticleLa L. Bruno A. Chiappetta M.B. Bitonti P. Bonfante O. Arrigoni (2004) ArticleTitleLocalization of ascorbic acidascorbic acid oxidaseand glutathione in roots of Cucurbita maxima L J. Exp. Bot. 55 2589–2597 Occurrence Handle10.1093/jxb/erh262 Occurrence Handle1:CAS:528:DC%2BD2cXhtVSqsr3L Occurrence Handle15520029
A. Metwally V.I. Safranova A.A. Belimov K. Dietz (2005) ArticleTitleGenotypic variation of the response to cadmium toxicity in Pisum sativum L J. Exp. Bot. 56 167–178 Occurrence Handle1:CAS:528:DC%2BD2MXkt1Wmtw%3D%3D Occurrence Handle15533881
N. Nautiyal C. Chatterjee C.P. Sharma (1999) ArticleTitleCopper stress affects grain filling in rice Commun. Soil Sci. Plant Anal. 30 1625–1632 Occurrence Handle1:CAS:528:DyaK1MXksl2mt7k%3D Occurrence Handle10.1080/00103629909370314
E. Olmos J.R. Martínez-Solano A. Piqueras E. Hellín (2003) ArticleTitleEarly steps in the oxidative burst induced by cadmium in cultured tobacco cells (BY-2 line) J. Exp. Bot. 54 291–301 Occurrence Handle10.1093/jxb/54.381.291 Occurrence Handle1:CAS:528:DC%2BD3sXhtlOiu7w%3D Occurrence Handle12493856
L. Perfus-Barbeoch N. Leonhardt A. Vavasseur C. Forestier (2002) ArticleTitleHeavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status Plant J. 32 539–548 Occurrence Handle10.1046/j.1365-313X.2002.01442.x Occurrence Handle1:CAS:528:DC%2BD38Xps1Kru7w%3D Occurrence Handle12445125
C. Pignocchi C.H. Foyer (2003) ArticleTitleApoplastic ascorbate metabolism and its role in the regulation of cell signaling Curr. Opin. Plant Biol. 6 379–389 Occurrence Handle10.1016/S1369-5266(03)00069-4 Occurrence Handle1:CAS:528:DC%2BD3sXlsValtbg%3D Occurrence Handle12873534
C. Pignocchi J.M. Fletcher J.E. Wilkinson J.D. Barnes C.H. Foyer (2003) ArticleTitleThe function of ascorbate oxidase in tobacco Plant Physiol. 132 1631–1641 Occurrence Handle10.1104/pp.103.022798 Occurrence Handle1:CAS:528:DC%2BD3sXlsFGhu7Y%3D Occurrence Handle12857842
G. Pitari G. Chichiricco G. Marcozzi A. Rossi M. Maccarrone L. Avigliano (1993) ArticleTitleExpression of ascorbate oxidase in cultured green zucchini cells – effect of copper and abscisic-acid Plant Physiol. Biochem. 31 593–598 Occurrence Handle1:CAS:528:DyaK2cXntlGltw%3D%3D
R.A. Reisfeld U.J. Levis D.E. Williams (1962) ArticleTitleDisk electrophoresis of basic proteins and peptides on polyacrylamide gels Nature 195 281–283 Occurrence Handle1:CAS:528:DyaF38XksVCjtbs%3D Occurrence Handle14491328
A. Rivetta N. Negrini M. Cocucci (1997) ArticleTitleInvolvement of Ca2+–calmodulin in Cd2+ toxicity during the early phases of radish (Raphanus sativus L.) seed germination Plant Cell Environ. 20 600–608 Occurrence Handle10.1111/j.1365-3040.1997.00072.x Occurrence Handle1:CAS:528:DyaK2sXjsFamurY%3D
M.C. Romero-Puertas J.M. Palma M. Gómez L.A. Del Río L.M. Sandalio (2002) ArticleTitleCadmium causes the oxidative modification of proteins in pea plants Plant Cell Environ. 25 677–686 Occurrence Handle10.1046/j.1365-3040.2002.00850.x Occurrence Handle1:CAS:528:DC%2BD38XktlertLg%3D
L. Sanita di Toppi R. Gabbrielli (1999) ArticleTitleResponse to cadmium in higher plants Environ. Exp. Bot. 41 105–130
M. Sanmartin P.D. Drogoudi T. Lyons I. Pateraki J. Barnes A.K. Kanellis (2003) ArticleTitleOver-expression of ascorbate oxidase in the apoplast of transgenic tobacco results in altered ascorbate and glutathione redox states and increased sensitivity to ozone Planta 216 918–928 Occurrence Handle1:CAS:528:DC%2BD3sXjvFGjsbg%3D Occurrence Handle12687359
R.K. Sarkar A. Das (2000) ArticleTitleChanges in anti-oxidative enzymes and anti-oxidants in relation to flooding tolerance in rice J. Plant Biol. 27 307–311
P. Schopfer C. Plachy G. Frahry (2001) ArticleTitleRelease of active oxygen intermediates (superoxide radicals, hydrogen peroxideand hydroxyl radicals) and peroxidase in germinating radish seeds controlled by lightgibberellin, and abscisic acid Plant Physiol. 125 1591–1602 Occurrence Handle10.1104/pp.125.4.1591 Occurrence Handle1:CAS:528:DC%2BD3MXjtFKqtrk%3D Occurrence Handle11299341
M. Šimonovičová J. Huttová I. Mistrík B. Široká L. Tamás (2004) ArticleTitlePeroxidase mediated hydrogen peroxide production in barley roots grown under stress conditions Plant Growth Regul. 44 267–275
N. Smirnoff (1996) ArticleTitleThe function and metabolism of ascorbic acid in plants Ann. Bot. 78 661–669 Occurrence Handle10.1006/anbo.1996.0175 Occurrence Handle1:CAS:528:DyaK2sXjtFKrtg%3D%3D
N. Smirnoff G.L. Wheeler (2000) ArticleTitleAscorbic acid in plants: biosynthesis and function Crit. Rev. Plant Sci. 19 267–290 Occurrence Handle1:CAS:528:DC%2BD3cXmtVCjs74%3D
L. Tamás M. Šimonovičová J. Huttová I. Mistrík (2004) ArticleTitleAluminium stimulated hydrogen peroxide production of germinating barley seeds Environ. Exp. Bot. 51 281–288
Y. Umemura T. Ishiduka R. Yamamoto M. Esaka (2004) ArticleTitleThe dof domain, a zinc finger DNA-binding domain conserved only in higher plants, truly function as a Cys2/Cys2 Zn finger domain Plant J. 37 741–749 Occurrence Handle10.1111/j.1365-313X.2003.01997.x Occurrence Handle1:CAS:528:DC%2BD2cXislylt7g%3D Occurrence Handle14871313
F. Wu J. Dong Q.Q. Qian G. Zhang (2005) ArticleTitleSubcellular distribution and chemical form of Cd and Cd–Zn interaction in different barley genotypes Chemosphere 60 1437–1446 Occurrence Handle1:CAS:528:DC%2BD2MXmvFSjt7g%3D Occurrence Handle16054913
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Tamás, L., Bočová, B., Huttová, J. et al. Cadmium-Induced Inhibition of Apoplastic Ascorbate Oxidase in Barley Roots. Plant Growth Regul 48, 41–49 (2006). https://doi.org/10.1007/s10725-005-4747-4
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DOI: https://doi.org/10.1007/s10725-005-4747-4