Abstract
Chrysolaena obovata (Less.) Dematt., a herbaceous Asteraceae species widely distributed across different Brazilian Cerrado physiognomies, bears underground organs, named rhizophores, that accumulate high concentrations of inulin-type fructans. These carbohydrates are recognized as beneficial soluble fibers for human health and are currently used in food and pharmaceutical industries. Considering that fructans, in addition to their economic potential, provide plants with greater tolerance to drought, heat and cold, it is important to understand whether their metabolism is conserved in natural populations. In this study, we investigated if the levels of genetic diversity in the studied populations allow selection of localities with high genetic base and high fructan content for future programs of in situ conservation and genetic improvement for inulin production. We characterized diversity, structure, and gene flow of seven natural populations from the Brazilian Cerrado, using nine microsatellite loci (SSR). In addition, we compared fructan composition between populations of different Cerrado phytophysiognomies. Overall, we found that C. obovata populations exhibited moderate levels of genetic diversity, low genetic differentiation, and high gene flow. This study identified two populations with lower genetic diversity (Santa Bárbara and Serra Dourada) than the remaining five, suggesting that greater attention should be given to conservation programs including these populations. Fructan metabolism is conserved in all populations, indicating that C. obovata is an important genetic resource with high potential for inulin production.
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Data availability
Data are deposited at Zenodo http://doi.org/10.5281/zenodo.6030291.
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Acknowledgements
This work is part of the PhD thesis of L.M.D. (Programa de Pós-Graduação em Biodiversidade Vegetal e Meio Ambiente, IBt). The authors thank Drs. Jimi Nakajima (Universidade Federal de Uberlândia) and Rosangela Simão-Bianchini (Instituto de Botânica) for species identification and Drs. Moemy Gomes de Moraes (Universidade Federal de Goiás), Cristiane da Silva Ferreira (Universidade Federal de Brasília), Jimi Nakajima (Universidade Federal de Uberlândia), Geraldo Aclecio Melo (Universidade Estadual de Montes Claros), and Giselda Durigan (Instituto Florestal de São Paulo) for their help with the logistics in plant collecting expeditions. Finally, we thank Dr. Rita de Cássia L. Figueiredo-Ribeiro for continuous support.
Funding
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CAPES/PNADB (Grant No 454/2010) financed field and laboratory work. L.M.D. received financial support from Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq (Grant No 478005/2011-3; 142410/2015-2). A.A-P was granted a post-doctoral scholarship by the Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP (Grant No 20018/00036-9), and M.A.M.C was a CNPq Research Fellow (Grant No 309635/2015-2).
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LMDC wrote the manuscript. LMD, MAMC and MG contributed to the conception and design of the study. LMD, MAMC and MG collected the plants from different populations in the field. AA-P and LMD obtained the data and carried out the bioinformatic analysis. All authors collaborated on analysis and interpretation of data, were involved in drafting, and revising the manuscript, approved the final version, and are accountable for all aspects of the work.
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Díaz, L.M., Alves-Pereira, A., Zucchi, M.I. et al. Chrysolaena obovata (Less.) Dematt., a species native of Brazilian Cerrado: genetic diversity and structure of natural populations and potential for inulin production. Genet Resour Crop Evol 70, 189–204 (2023). https://doi.org/10.1007/s10722-022-01425-3
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DOI: https://doi.org/10.1007/s10722-022-01425-3