Abstract
Male sterility is widely utilized for hybrid seed production. In this study, two new found male sterile mutants SauS4 and SauS5 were obtained from space flighted seeds of maize inbred line RP125. Then, genetic analysis, molecular markers identification, and cytological observation were conducted to confirm their male sterile types. For genetic analysis, the above two male sterile mutants were continuously backcrossed with two maize inbred line 18Hong and RP125, and four stable male sterile lines SauS4(18Hong), SauS5(18Hong), SauS4(RP125), SauS5(RP125) were generated by six-generation backcross. Restoring and maintaining relationship analysis showed that both Hui313 and Zifeng1 didn’t rescue the male sterility SauS4(18Hong) and SauS5(18Hong). Using CMS mitochondria-specific primers for PCR detection suggested that only a 440 bp band unique to CMS-T type was amplified in SauS4(18Hong), SauS5(18Hong), SauS4(RP125), and SauS5(RP125). Sequencing results showed that these bands sequences were identical in DNA level which compared with T-urf13. Cytological observations showed that the main abortion stages of SauS4 and SauS5 were at the middle stage of uninucleate microspores under the two nuclear backgrounds of 18Hong and RP125, exhibiting the characteristics of sporophyte sterility. All the above results pointed out the two male sterile mutants SauS4 and SauS5 belonged to the CMS-T type. Interestingly, some mitochondrial genome difference between SauS4(RP125) and SauS5(RP125) were revealed by AFLP analysis.
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References
Allen JO, Fauron CM, Patrick M et al (2007) Comparisons among two fertile and three male-sterile mitochondrial genomes of maize. Genetics 177(2):1173–1192. https://doi.org/10.1534/genetics.107.073312
Beckett JB (1971) Classification of male-sterile cytoplasms in maize (Zea mays L.). Crop Sci 11:724–727. https://doi.org/10.1007/BF02862061
Cao MJ, Rong TZ, Pan GT (2003) Genetic analysis about maize male sterile mutant obtained by space flight. (China). Acta Genet Sin 30:817–822. https://doi.org/10.1023/A:1022289509702(in Chinese with English abstract)
Castiglioni P, Marsan PA, Wijk RV, Motto M (1999) AFLP markers in a molecular linkage map of maize: codominant scoring and linkage group ditsribution. Theor Appl Genet 99(3–4):425–431. https://doi.org/10.1007/s001220051253
Charles S, Levings I (1990) The texas cytoplasm of maize: cytoplasmic male sterility and disease susceptibility. Science 250:942–947. https://doi.org/10.1126/science.250.4983.942
Dewey RE, Timothy DH, Levings CS (1992) Chimeric mitochondrial genes expressed in the C male-sterile cytoplasm of maize. Curr Genet 20:475–482. https://doi.org/10.1007/BF00334775
Feng Y, Zheng Q, Song H, Wang Y et al (2015) Multiple loci not only Rf3 involved in the restoration ability of pollen fertility, anther exsertion and pollen shedding to S type cytoplasmic male sterile in maize. Theor Appl Genet 128:2341–2350. https://doi.org/10.1007/s00122-015-2589-7
Geleta LF, Labuschagne MT, Viljoen CD (2008) Relationship between heterosis and genetic distance based on morphological traits and AFLP markers in pepper. Plant Breed 123(5):467–473. https://doi.org/10.1111/j.1439-0523.2004.01017.x
Guo BJ, Zhang ZX, Chen BT, Liu BS (2008) Genetic analysis of male sterile cytoplasm of Ta-CMS in maize. (China). Mol Plant Breed 6:491–494. https://doi.org/10.1145/1344411.1344416(in Chinese with English abstract)
Liu ZY, Peter SO, Long M et al (2002) A PCR assay for rapid discrimination of cytoplasm types in maize. Crop Sci 42:566–569. https://doi.org/10.2135/cropsci2002.5660
Mackill DJ, Zhang Z, Redona ED, Colowit PM (1996) Level of polymorphism and genetic mapping of AFLP markers in rice. Genome 39(5):969–977. https://doi.org/10.1139/g96-121
Mao CZ, Yi KK, Yang Y et al (2004) Identification of aluminium-regulated genes by cDNA-AFLP in rice (Oryza sativa L.): aluminium-regulated genes for the metabolism of cell wall components. J Exp Bot 55(394):137–143. https://doi.org/10.1093/jxb/erh030
Prins R, Groenewald JZ, Marais GF et al (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor Appl Genet 103(4):618–624. https://doi.org/10.1007/pl00002918
Rong TZ, Li WC, Cao MJ, Hu CY (2002) Study on identification in group of cytoplasmic male sterile in maize. (China). Sci Agric Sin 35:1055–1059. https://doi.org/10.1006/jfls.2001.0409(in Chinese with English abstract)
Vos P, Hogers R, Bleeker M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414. https://doi.org/10.1093/nar/23.21.4407
Wang C, Li HY, Zhang LY, Pei YX, Wang YQ (2013) Identification of an AFLP marker and conversion to a SCAR marker to identify cytoplasmic male-sterile or normal cytoplasm in Welsh onion (Allium fistulosum L.). J Hort Sci Biotechnol 88:409–414. https://doi.org/10.1080/14620316.2013.11512984
Wang J, Cao MJ, Pan GT, Lu YL, Rong TZ (2009) RNA editing of mitochondrial functional genes atp6 and cox2 in maize (Zea mays L.). Mitochondrion 9:364–369. https://doi.org/10.1016/j.mito.2009.07.005
Warmke HE, Lee J (1977) Mitochondrial degeneration in Texas cytoplasmic male-sterile corn anthers. J Hered 68:213–222. https://doi.org/10.1093/oxfordjournals.jhered.a108817
Xia T, Liu JL (1989) The cytological study of cytoplasmic male sterility in maize. (China). Acta Agron Sin 2:97–103 (in Chinese with English abstract)
Xu XB, Yin WJ, Xing YJ et al (2013) Genetic analysis of maize male sterile mutant obtained by space flight. (China). Shandong Agric Sci 45:28–31. https://doi.org/10.14083/j.issn.1001-4942.2013.04.029(in Chinese with English abstract)
Zabala G, Susan G, John RL (1997) The nuclearg Rf3 affects the expression of the mitochondrial chimeric sequence R implicated in S-type male sterility in maize. Genetics 147:847–860. https://doi.org/10.1017/S0016672397002966
Zhang CB, Yuan GZ, Wang J et al (2011) Genetic analysis of maize cytoplasmic male sterile mutants obtained by space flight. (China). Hereditas 33:175–181. https://doi.org/10.3724/SP.J.1005.2011.00175(in Chinese with English abstract)
Zhang ZX, Fang MJ, Du HW, Deng LR, Zheng YL (2005) The rapid discrimination based on PCR on cytoplasmic types of male sterile line of maize (Zea mays L.). (China). Acta Agron Sin 31:1386–1388. https://doi.org/10.3321/j.issn:0496-3490.2005.10.027(in Chinese with English abstract)
Zhao ZF, Huang L, Liu YM et al (2018) Genetics of fertility restoration in the isocytoplasm allonuclear C-group of cytoplasmic male sterility in maize. (China). Hereditas 40:402–414. https://doi.org/10.16288/j.yczz.17-401(in Chinese with English abstract)
Zheng YL (1982) Mechanism of several CMS maize. (China). J Huazhong Agric Univ 1:44–68. https://doi.org/10.13300/j.cnki.hnlkxb.1982.01.005(in Chinese with English abstract)
Funding
This research was supported by the National Key Research and Development Program of China (No. 2016 YFD0102104), Platform for Mutation Breeding by Radiation in Sichuan (No. 2016NZ0106), and Key Project of Education Development of Sichuan Province (No. 035Z2246).
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HY conceived work in this study together with CZ, CL, JW, TY. YL performed the analysis. HY wrote the manuscript initial of manuscript, MC modified and approved this manuscript.
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Yi, H., Zhang, C., Li, C. et al. Identification and genetic analysis of two maize CMS-T mutants obtained from out-space-flighted seeds. Genet Resour Crop Evol 68, 1937–1947 (2021). https://doi.org/10.1007/s10722-021-01107-6
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DOI: https://doi.org/10.1007/s10722-021-01107-6