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Somatic cybridization for Citrus: polyphenols distribution in juices and peel essential oil composition of a diploid cybrid from Cleopatra mandarin (Citrus reshni Hort. ex Tan.) and sour orange (Citrus aurantium L.)

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Abstract

Somatic cybridization is a technique based on inducted or spontaneous fusion between nuclear genome of one parent and either the cytoplasmic genome of the other parent or a combination of both donors. This approach is considered a powerful biotechnological tool in plant improvement since it enables to obtain novel intergenomic recombinations that are impossible to achieve by conventional crosses. Twenty-five plants were regenerated following protoplast fusion of Cleopatra mandarin (Citrus reshni Hort. ex Tan.) and sour orange (Citrus aurantium L.) and a new 2n cybrid has been identified (4 % of plants obtained). A complete phytochemical investigation of the new genotype and both parents was carried out analyzing the polyphenol content of juices by liquid-chromatography–ultraviolet–diode array detector–mass spectrometry and the peel essential oils using a combination of gas chromatography equipped with a flame ionization detector and a mass spectrometry. On the whole 10 flavonoids (7 flavanone and 3 flavones) and 4 hydroxycinnamic acids were detected and quantified, while 67 components were fully characterized and grouped into four classes (monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpenes, and others) for an easier comparison of all oils. Comparing the new cybrid to both parents an increase of the monoterpene hydrocarbons and a corresponding decrease of oxygenated components were shown. The aim of the present work was to evaluate whether the genome rearrangements in the new cybrid, obtained through not conventional strategies, can produce superior traits and improved performance such as enhanced presence and quality of bioactive components.

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References

  • Abbate L, Tusa N, Fatta Del Bosco S, Strano T, Renda A, Ruberto G (2012) Genetic improvement of Citrus fruits: new somatic hybrids from Citrus sinensis (L.) Osb. and Citrus limon (L.) Burm. f. Food Res Int 48:284–290

    Article  CAS  Google Scholar 

  • Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry, 4th edn. Allured Publishing Corporation, Carol Stream

    Google Scholar 

  • Amirsadeghi S, Robson CA, Vanlerberghe GC (2007) The role of the mitochondrion in plant responses to biotic stress. Physiol Plant 129:253–266

    Article  CAS  Google Scholar 

  • Atienza SG, Martín A, Pecchioni N, Platani C, Cattivelli L (2008) The nuclear–cytoplasmic interaction controls carotenoid content in wheat. Euphytica 159:325–331

    Article  CAS  Google Scholar 

  • Barkley NA, Roose ML, Krueger RR, Federici CT (2006) Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor Appl Genet 112:1519–1531

    Article  CAS  PubMed  Google Scholar 

  • Barreca D, Bellocco E, Caristi C, Leuzzi U, Gattuso G (2011) Distribution of C- and O-glycosyl flavonoids, (3-hydroxy-3-methylglutaryl) glycosyl flavanones and furocoumarins in Citrus aurantium L. juice. Food Chem 124:576–582

    Article  CAS  Google Scholar 

  • Bassene J-B, Berti L, Carcouet E, Dhuique-Mayer C, Fanciullino A-L, Bouffin J, Ollitrault P, Froelicher Y (2008) Influence of mitochondria origin on fruit quality in a citrus cybrid. J Agric Food Chem 56:8635–8640

    Article  CAS  PubMed  Google Scholar 

  • Bassene J-B, Froelicher Y, Navarro L, Ollitrault P, Ancillo G (2011) Influence of mitochondria on gene expression in a citrus cybrid. Plant Cell Rep 30:1077–1085

    Article  CAS  PubMed  Google Scholar 

  • Benavente-García O, Castillo J (2008) Update on uses and properties of Citrus flavonoids: new findings in anticancer, cardiovascular, and anti-inflammatory activity. J Agric Food Chem 56:6185–6205

    Article  PubMed  Google Scholar 

  • Cheng Y, Guo W, Deng X (2003) cpSSR: a new tool to analyse chloroplast genome of Citrus somatic hybrids. Acta Bot Sin 45:906–909

    CAS  Google Scholar 

  • Cheng Y, De Vicente MC, Meng H, Guo W, Tao N, Deng X (2005) A set of primers for analyzing chloroplast DNA diversity in Citrus and related genera. Tree Physiol 25:661–672

    Article  PubMed  Google Scholar 

  • Dinakar C, Abhaypratap V, Yearla SR, Raghavendra AS, Padmasree K (2010) Importance of ROS and antioxidant system during the beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation. Planta 231:461–474

    Article  CAS  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Fabroni S, Ruberto G, Rapisarda P (2012) Essential oil profiles of new citrus hybrids, a tool for genetic citrus improvement. J Essent Oil Res 24:159–169

    Article  CAS  Google Scholar 

  • Fanciullino A-L, Gancel A, Froelicher Y, Luro F, Ollitrault P, Brillouet JM (2005) Effects of nucleo-cytoplasmic interactions on leaf volatile compounds from citrus somatic diploid hybrids. J Agric Food Chem 53:4517–4523

    Article  CAS  PubMed  Google Scholar 

  • Fatta Del Bosco S, Abbate L, Tusa N, Strano T, Renda A, Ruberto G (2013) Genetic improvement of Citrus fruits: the essential oil profiles in a Citrus limon backcross progeny derived from somatic hybridization. Food Res Int 50:344–350

    Article  CAS  Google Scholar 

  • Froelicher Y, Mouhaya W, Bassene J, Costantino G, Kamiri M, Luro F, Morillon R, Ollitrault P (2011) New universal mitochondrial PCR markers reveal new information on maternal citrus phylogeny. Tree Genet Genomes 7:49–61

    Article  Google Scholar 

  • Galbraith DW, Lambert GM, Macas J, Doležel J (1998) Analysis of nuclear DNA content and ploidy in higher plants. In: Robinson JP, Darzynkiewicz Z, Dean PN, Dressler LG, Orfao A, Rabinovitch PS, Stewart CC, Tanke HJ, Wheeless LL (eds) Current protocols in cytometry. Wiley, New York, pp 7.6.1–7.6.22

    Google Scholar 

  • Gancel AL, Ollitrault P, Froelicher Y, Tomi F, Jacquemond C, Luro F, Brillouet J-M (2003) Leaf volatile compounds of seven citrus somatic tetraploid hybrids sharing willow leaf mandarin (Citrus deliciosa Ten.) as their common parent. J Agric Food Chem 51:6006–6013

    Article  CAS  PubMed  Google Scholar 

  • Gancel A-L, Ollitrault P, Froelicher Y, Tomi F, Jacquemond C, Luro F, Brillouet J-M (2005) Citrus somatic allotetraploid hybrids exhibit a differential reduction of leaf sesquiterpenoid biosynthesis compared with their parents. Flavour Fragr J 20:626–632

    Article  CAS  Google Scholar 

  • Grosser JW, Gmitter FG Jr (2011) Protoplast fusion for production of tetraploids and triploids: applications for scion and rootstock breeding in citrus. Plant Cell Tissuebiotechnology of plant organelles Org 104:343–357

    Article  CAS  Google Scholar 

  • Grosser JW, Gmitter FG Jr, Tusa N, Reforgiato Recupero G, Cucinotta P (1996) Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts. Plant Cell Rep 15:672–676

    Article  CAS  PubMed  Google Scholar 

  • Grosser JW, Ollitrault P, Olivares-Fuster O (2000) Somatic hybridization in citrus: an effective tool to facilitate variety improvement. In Vitro Cell Dev Plant 36:434–449

    Article  Google Scholar 

  • Guo WW, Cai XD, Grosser JW (2004) Somatic cell cybrids and hybrids in plant improvement. In: Daniel H, Chase CD (eds) Molecular biology and biotechnology of plant organelles. Kluwer, Dordrecht, pp 635–659

    Chapter  Google Scholar 

  • Guo WW, Cheng Y, Chen C, Deng X (2006) Molecular analysis revealed autotetraploid, diploid and tetraploid cybrid plants regenerated from an interspecific somatic fusion in Citrus. Sci Hortic 108:162–166

    Article  CAS  Google Scholar 

  • Guo WW, Wu RC, Cheng YJ, Deng XX (2007) Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange. Plant Breed 126:72–76

    Article  CAS  Google Scholar 

  • Guo WW, Xiao SX, Deng XX (2013) Somatic cybrid production via protoplast fusion for citrus improvement. Sci Hortic 163:20–26

    Article  Google Scholar 

  • Hamdan DI, Abdulla RH, Mohamed ME, El-Shazly AM (2013) Chemical composition and biological activity of essential oils of Cleopatra mandarin (Citrus reshni) cultivated in Egypt. J Pharmacogn Phytother 5(5):83–90

    CAS  Google Scholar 

  • Iovene M, Savarese S, Cardi T, Frusciante L, Scotti N, Simon PW, Carputo D (2007) Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids. Genome 50:443–450

    Article  CAS  PubMed  Google Scholar 

  • Jannati M, Fotouhi R, Abad AP, Salehi Z (2009) Genetic diversity analysis of Iranian citrus varieties using micro-satellite (SSR) based markers. J Hortic For 1:120–125

    Google Scholar 

  • Johnson AAT, Veilleux RE (2001) Somatic hybridization and applications in plant breeding. Plant Breed Rev 20:167–225

    CAS  Google Scholar 

  • Jones P, Keane EM, Osborne BA (1998) Effects of alien cytoplasmic variation on carbon assimilation and productivity in wheat. J Exp Bot 49:1519–1528

    Article  CAS  Google Scholar 

  • Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, Griel AE, Etherton TD (2002) Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med 113 (Suppl 9B):71S–88S

    Article  CAS  PubMed  Google Scholar 

  • Liu Z, Butow RA (2006) Mitochondrial retrograde signaling. Ann Rev Genet 40:159–185

    Article  CAS  PubMed  Google Scholar 

  • Lota M-L, de Rocca SD, Tomi F, Casanova J (2001) Chemical variability of peel and leaf essential oils of 15 species of mandarins. Biochem Syst Ecol 29:77–104

    Article  CAS  PubMed  Google Scholar 

  • Matsui K, Yoshida M, Ban T, Komatsuda T, Kawada N (2002) Role of male-sterile cytoplasm in resistance to barley yellow mosaic virus and Fusarium head blight in barley. Plant Breed 121:237–240

    Article  Google Scholar 

  • Moreira CD, Chase CD, Gmitter FG Jr, Grosser JW (2000) Inheritance of organelle genomes in citrus somatic cybrids. Mol Breed 6:401–405

    Article  CAS  Google Scholar 

  • Nichenametla SN, Taruscio TG, Barney DL, Exon JH (2006) A review of the effects and mechanism of polyphenolics in cancer. Crit Rev Food Sci Nutr 46:161–183

    Article  CAS  PubMed  Google Scholar 

  • NIST (1998) National Institute of Standard and Technology. Mass Spectral Library (Version 1998)

  • Partec (2015). Symsex Europe GmbH, Germany. http://www.sysmex-europe.com/partec

  • Ram MU, Guddadarangavvanahally KJ, Bhimanagouda SP (2011) Rapid separation method of polymethoxyflavones from citrus using flash chromatography. Sep Purif Technol 81:151–158

    Article  Google Scholar 

  • Rapisarda P, Pannuzzo P, Romano G, Russo G (2003) Juice components of a new pigmented Citrus hybrid Citrus sinensis (L.) Osbeck × Citrus clementina Hort. ex Tan. J Agric Food Chem 51:1611–1616

    Article  CAS  PubMed  Google Scholar 

  • Rhoads DM, Subbaiah CC (2007) Mitochondrial retrograde regulation in plants. Mitochondrion 7:177–194

    Article  CAS  PubMed  Google Scholar 

  • Ruberto G, Starrantino A, Rapisarda P (1999) Citrus improvement—chemical and genetic aspects. In: Pandalai SG (ed) Recent research developments in agricultural and food chemistry. Research Signpost, Trivandrum, pp 445–470

    Google Scholar 

  • Saito W, Ohgawara T, Shimizu J, Ishii S, Kobayashi S (1993) Citrus cybrid regeneration following cell fusion between nucellar cells and mesophyll cells. Plant Sci 88:195–201

    Article  CAS  Google Scholar 

  • Shonnard GC, Gepts P (1994) Genetics of heat tolerance during reproductive development in common bean. Crop Sci 34:1168–1175

    Article  Google Scholar 

  • Siragusa M, Carra A, Salvia L, Puglia AM, De Pasquale F, Carimi F (2007) Genetic instability in calamondin (Citrus madurensis Lour.) plants derived from somatic embryogenesis induced by diphenylurea derivative. Plant Cell Rep 26:1289–1296

    Article  CAS  PubMed  Google Scholar 

  • Strand A (2004) Plastid-to-nucleus signaling. Curr Opin Plant Biol 7:621–625

    Article  CAS  PubMed  Google Scholar 

  • Suzuki N, Koussevitzky S, Mitter R, Miller G (2002) ROS and redox signaling in the response of plants to abiotic stress. Plant Cell Environ 35:259–270

    Article  Google Scholar 

  • Szajdek A, Borowska EJ (2008) Bioactive compounds and health-promoting properties of berry fruits: a review. Plant Food Hum Nutr 63:147–156

    Article  CAS  Google Scholar 

  • Tripoli E, La Guardia M, Giammanco S, Di Majo D, Giammanco M (2007) Citrus flavonoids: molecular structure, biological activity and nutritional properties: a review. Food Chem 104:466–479

    Article  CAS  Google Scholar 

  • Tusa N, Fatta Del Bosco S, Nigro F, Ippolito A (2000) Response of cybrids and a somatic hybrid of lemon to Phoma tracheiphila infections. HortScience 35:125–127

    Google Scholar 

  • Tusa N, Abbate L, Renda A, Ruberto G (2007) Polyphenol distribution in juices from Citrus allotetraploid somatic hybrids and their sexual hybrids. J Agric Food Chem 55:9089–9094

    Article  CAS  PubMed  Google Scholar 

  • Uprety DC, Tomar VK (1993) Photosynthesis and drought resistance of Brassica carinata and its parent species. Photosynthetica 29:321–327

    Google Scholar 

  • Wang L, Pan ZY, Guo WW (2010) Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo. Plant Cell Tissue Org 103:165–174

    Article  CAS  Google Scholar 

  • Wang J, Jiang J, Wang Y (2013) Protoplast fusion for crop improvement and breeding in China. Plant Cell Tissue Org 112:131–142

    Article  Google Scholar 

  • Woodson JD, Chory J (2008) Coordination of gene expression between organellar and nuclear genomes. Nat Rev Genet 9:383–395

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yamamoto M, Matsumoto R, Okudai N, Yamada Y (1997) Aborted anthers of Citrus result from gene–cytoplasmic male sterility. Sci Hortic 70:9–14

    Article  Google Scholar 

  • Zhang A, Yu F, Zhang F (2003) Alien cytoplasm effects on phytosiderophore release in two spring wheats (Triticum aestivum L.). Gen Res Crop Evol 50:767–772

    Article  CAS  Google Scholar 

  • Zubko MK, Zubko EI, Adler K, Grimm B, Gleba YY (2003) New CMS phenotypes in cybrids Nicotiana tabacum L. (Hyoscyamus niger L.). Ann Bot Lond 92:281–288

    Article  Google Scholar 

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Acknowledgments

The authors wish to thank Mrs. Tonia Strano, Mr. Agatino Renda (ICB-CNR, Catania) and Dr. Antonio Motisi (IBBR-CNR, Palermo) for their skilful technical assistance. This work was financially supported by Consiglio Nazionale delle Ricerche (CNR, Rome, Italy).

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    Authors and Affiliations

    1. CNR - Istituto di Bioscienze e BioRisorse, Corso Calatafimi 414, 90129, Palermo, Italy

      Sergio Fatta Del Bosco, Francesco Mercati, Loredana Abbate & Francesco Carimi

    2. CNR - Istituto di Chimica Biomolecolare, Via Paolo Gaifami 18, 95126, Catania, Italy

      Edoardo Napoli & Giuseppe Ruberto

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    1. Sergio Fatta Del Bosco
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    2. Edoardo Napoli
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    3. Francesco Mercati
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    Correspondence to Francesco Mercati or Giuseppe Ruberto.

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    Sergio Fatta Del Bosco and Edoardo Napoli have equally contributed to this work.

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    Fatta Del Bosco, S., Napoli, E., Mercati, F. et al. Somatic cybridization for Citrus: polyphenols distribution in juices and peel essential oil composition of a diploid cybrid from Cleopatra mandarin (Citrus reshni Hort. ex Tan.) and sour orange (Citrus aurantium L.). Genet Resour Crop Evol 64, 261–275 (2017). https://doi.org/10.1007/s10722-015-0348-x

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