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Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins

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Abstract

We present the purification and characterization of the two most abundant isoforms of lectins isolated from Tepary bean (Phaseolus acutifolius) seeds, which have been shown to differentially affect the survival of different cancer cells. They were separated by concanavalin A-affinity chromatography. After purification, to release the N-glycans, they were digested with the endoglycosidases PNGase and Glycanase A. Fractions resulted from the hydrolysis products were analyzed to determine their carbohydrate composition. Mass spectrometry data indicated that both isoforms contained high mannose glycans being mannose 6 the most abundant form. Furthermore, based on sequence Ans-X-Ser/Thr, where X is any amino acid except proline, a glycosylation site was determined on asparagine 36. When their metal requirement to preserve their biological activity was determined, the lectins showed differences. While lectin A (LA) agglutination activity was best in the presence of magnesium, lectin B (LB) was best with calcium. Additionally, only LA exhibited affinity to human type-A erythrocytes. Although both lectins showed small differences in their properties, an identical structure-model for both lectins was generated by the homology modelling process. Also, the analysis of ligand binding sites and in silico glycosylation were achieved. Molecular docking with colon adenocarcinoma associated-N-glycans revealed some highly possible interactions and, on the other hand, that N-glycan interaction zones of Tepary bean lectins is not restricted to the carbohydrate binding domain but to an extended part of their surface, which could lead new strategies to explain their biological activity.

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Funding

Funding was provided by Consejo Nacional de Ciencia y Tecnología [project number CB-2014–01241181].

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Authors and Affiliations

  1. Tecnológico Nacional de México / Instituto Tecnológico Superior de Irapuato, Carretera Irapuato-Silao Km. 12.5. Colonia El Copal, 36821, Irapuato, Guanajuato, México

    José Luis Castro-Guillén

  2. Centro de Investigación y de Estudios Avanzados. Unidad Irapuato. Departamento de Biotecnología y Bioquímica., Libramiento Norte. Carretera Irapuato-León. Km. 9.6, 36824, Irapuato, Guanajuato, México

    Iovanna Torres-Arteaga, Alejandro Blanco-Labra, Elizabeth Mendiola-Olaya & Alondra L. Ortega-de-Santiago

  3. Universidad Autónoma de Querétaro. Campus Juriquilla. Facultad de Ciencias Naturales., Av. de las Ciencias s/n, Juriquilla, 76230, Santiago de Querétaro, Querétaro, México

    Teresa García-Gasca

  4. Tecnológico Nacional de México / Instituto Tecnológico de Roque., Carretera Celaya-Juventino Rosas Km. 8., 38110, Celaya, Guanajuato, México

    Cesar Aguirre-Mancilla

  5. University of California. Davis campus. Department of Chemistry, One Shields Ave. Chemistry Department 2465. Chemistry Annex., 95616, CA, Davis, USA

    Mariana Barboza & Carlito B. Lebrilla

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  1. Iovanna Torres-Arteaga
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  2. Alejandro Blanco-Labra
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  9. José Luis Castro-Guillén
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Contributions

All authors contributed to the study conception and design. Material preparation, purification, and characterization of Tepary bean lectins, data collection and analysis were performed by Iovanna Torres-Arteaga, José Luis Castro-Guillén, Elizabeth Mendiola-Olaya, Teresa García-Gasca, Cesar Aguirre-Mancilla, Alondra L. Ortega-de-Santiago, and Alejandro Blanco-Labra. Carbohydrate identification and analysis were performed by Iovanna Torres-Arteaga, Mariana Barboza, and Carlito B. Lebrilla. Homology modelling, in silico glycosylation, and docking analysis was achieved by José Luis Castro-Guillén. The first draft of the manuscript was written by Iovanna Torres-Arteaga, José Luis Castro-Guillén, Elizabeth Mendiola-Olaya, and Alejandro Blanco-Labra, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to José Luis Castro-Guillén.

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Torres-Arteaga, I., Blanco-Labra, A., Mendiola-Olaya, E. et al. Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins. Glycoconj J 40, 69–84 (2023). https://doi.org/10.1007/s10719-022-10091-7

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