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Eryptosis in hereditary spherocytosis and thalassemia: role of glycoconjugates

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Abstract

The present work is aimed to study the mechanism of faster erythrocyte clearance in hereditary spherocytosis (HS), a heterogeneous disorders characterized by alterations in the proteins of the red cell membrane skeleton along with different kinds of thalassemia. The maximum exposure of phosphatidylserine (PS) is found in HS compared to those in both α- and β-thalassemia. Interestingly, in HS more PS exposed cells were found in younger erythrocytes compared to normal and the thalassemics where aged cells showed higher loss of PS asymmetry. Loss of sialic acid and GlcNAc bearing glycoconjugates, presumably the glycophorins, was also found upon aging. The loss of PS asymmetry together with the cell surface glycoproteins mediated by membrane vesiculation, seemed to play key role in early clearance of erythrocytes from circulation following a mechanism similar to HbEβ-thalassemia.

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Abbreviations

HS:

Hereditory spherocytosis

HE:

Hereditory elliptocytosis

PS:

Phosphatidylserine

FITC:

Fluorescein-5-isothiocyate

AV-FITC:

Fluorescein-5-isothiocyate labeled Annexin V

WGA-FITC:

Fluorescein-5-isothiocyate labeled Wheat Germ Agglutinin

PWM-FITC:

Fluorescein-5-isothiocyate labeled Poke Weed Mitogen

Ca2+/A23187:

1 mM Ca2+ with 4 μM of A23187

GlcNAc:

N-acetyl-D-glucosamine

Neu5Ac:

N-acetyl-D-neuraminic acid

MFI:

Mean fluorescence intensity

SEM:

Standard error of the mean

PBS:

Phosphate buffered saline containing 2.7 mM KCl, 1.5 mM KH2PO4, 137 mM NaCl, 8.1 mM Na2HPO4, 0.01% NaN3 pH 7.4

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Authors and Affiliations

  1. Biophysics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700064, India

    Sumanta Basu & Abhijit Chakrabarti

  2. Structural Genomics Section, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700064, India

    Abhijit Chakrabarti

  3. Department of Pathology, Ramakrishna Mission Seva Prathisthan, Kolkata, 700026, India

    Debasis Banerjee

  4. Department of Hemato-Oncology, Kothari Medical Centre, Kolkata, 700027, India

    Sarmila Chandra

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  1. Sumanta Basu
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  2. Debasis Banerjee
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  3. Sarmila Chandra
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  4. Abhijit Chakrabarti
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Correspondence to Abhijit Chakrabarti.

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Basu, S., Banerjee, D., Chandra, S. et al. Eryptosis in hereditary spherocytosis and thalassemia: role of glycoconjugates. Glycoconj J 27, 717–722 (2010). https://doi.org/10.1007/s10719-009-9257-6

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  • DOI: https://doi.org/10.1007/s10719-009-9257-6

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