Skip to main content
Log in

Meiotic chromosome configurations in triploid progeny from reciprocal crosses between wild-type diploid and natural tetraploid loach Misgurnus anguillicaudatus in China

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

Here, we showed meiotic chromosome configurations prepared from oocyte germinal vesicles and spermatocytes of triploid loaches produced from reciprocal crosses between wild-type diploids (2n = 50) obtained from Dalian, Liaoning Province, China and natural tetraploids (4n = 100) from Chibi, Hubei Province, China. Major meiotic cells in triploids comprised 25 bivalents and 25 univalents, but cells with one to five trivalents were also observed. When three homologous chromosomes bearing nucleolar organizing regions (NOR) were identified with the detection of signals or positive sites by silver staining, chromomycin A3 staining and fluorescence in situ hybridization with a 5.8S + 28S rDNA probe, two third of selected triploid cells gave a configuration including one bivalent with two NORs (association of two homologous chromosomes) and one univalent with one NOR. However, other triploid cells showed three univalent each of which had one NOR, suggesting a failure of synapsis between homologous chromosomes. These results suggested that triploid female and male should produce aneuploid gametes with the theoretical mode at 1.5n (37 or 38 chromosomes).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Arai K, Fujimoto T (2013) Genomic constitution and atypical reproduction in polyploidy and unisexual lineages of the Misgurnus loach, a teleost fish. Cytogenet Genome Res 140:226–240

    Article  CAS  PubMed  Google Scholar 

  • Arai K, Mukaino M (1997) Clonal nature of gynogenetically induced progeny of triploid (diploid × tetraploid) loach Misgurnus anguillicaudatus (Pisces: Cobitididae). J Exp Zool 278:412–420

    Article  CAS  Google Scholar 

  • Arai K, Mukaino M (1998) Electrophoretic analysis of the diploid progenies from triploid × diploid crosses in the loach Misgurnus anguillicaudatus (Pisces: Cobitidae). J Exp Zool 280:368–374

    Article  Google Scholar 

  • Benfey TJ (1999) The physiology and behavior of triploid fishes. Rev Fish Sci 7(1):39–67

    Article  Google Scholar 

  • Benfey TJ (2011) Physiology of triploid fish. In: Farrell AP (ed) Encyclopedia of Fish Physiology: From Genome to Environment, vol 3. Academic Press, San Diego, pp 2009–2015

    Chapter  Google Scholar 

  • Cherfas NB, Gomelsky B, Ben-Dom N, Peretz Y, Hulata G (1994) Assessment of triploid common carp (Cyprinus carpio L.) for culture. Aquaculture 127:11–18

    Article  Google Scholar 

  • Davisson MT, Wright JE, Aterton LM (1973) Cytogenetic analysis of pseudolinkage of LDH loci in the teleost genus Salvelinus. Genetics 73:645–658

    PubMed Central  CAS  PubMed  Google Scholar 

  • Gomelsky B, Schneider KJ, Anil A, Delomas TA (2015) Gonad development in triploid ornamental koi carp and results of crossing triploid females with diploid males. N Am J Aquac 77:96–101

    Article  Google Scholar 

  • Itono M, Morishima K, Fujimoto T, Bando E, Yamaha E, Arai K (2006) Premeiotic endomitosis produces diploid eggs in the natural clone loach, Misgurnus anguillicaudatus (Teleostei: Cobitidae). J Exp Zool A Comp Exp Biol 305A:513–523

    Article  CAS  Google Scholar 

  • Itono M, Okabayashi N, Morishima K, Fujimoto T, Yoshikawa H, Yamaha E, Arai K (2007) Cytological mechanisms of gynogenesis and sperm incorporation in unreduced diploid eggs of the clonal loach, Misgurnus anguillicaudatus (Teleostei: Cobitidae). J Exp Zool A Ecol Genet Phsiol 307A:35–50

    Article  CAS  Google Scholar 

  • Lee GM, Wright JE (1981) Mitotic and meiotic analyses of brook trout Salvelinus fontinalis. J Hered 72:321–327

    Google Scholar 

  • Li YJ, Yin J, Wang JB, Yuan X, Wei J, Sun XW, Arai K (2008) A Study on the distribution of polyploid loaches in China. Nippon Suisan Gakkaishi 74:177–182

    Article  Google Scholar 

  • Li YJ, Tian Y, Zhang MZ, Tian PP, Yu Z, Abe S, Arai K (2010) Chromosome banding and FISH with an rDNA probe in the diploid and tetraploid Loach. Misgurnus anguillicaudatus. Ichthyol Res 57(4):358–366

    Article  Google Scholar 

  • Li YJ, Yu Z, Zhang MZ, Qian C, Abe S, Arai K (2011) The origin of natural tetraploid loach Misgurnus anguillicaudatus (Teleostei:Cobitidae) inferred from meiotic chromosome configurations. Genetica 139:805–811

    Article  PubMed  Google Scholar 

  • Li YJ, Zhang MZ, Qin C, Gao M, Arai K (2012) Fertility and ploidy of gametes of diploid, triploid and tetraploid loaches, Misgurnus anguillicaudatus, in China. J Appl Ichthyol 28:900–905

    Article  Google Scholar 

  • Li YJ, Yu Z, Zhang MZ, Qian C, Abe S, Arai K (2013) Induction and viable gynogenetic progeny using eggs and UV-irradiated sperm from the Chinese tetraploid loach, Misgurnus anguillicaudatus. Aquacult Int 21:759–768

    Article  Google Scholar 

  • Matsubara K, Arai K, Suzuki R (1995) Survival potential and chromosomes of progeny of triploid and pentaploid females in the loach Misgurnus anguillicaudatus. Aquaculture 131:37–48

    Article  Google Scholar 

  • Morishima K, Horie S, Yamaha E, Arai K (2002) A cryptic clonal line of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) evidenced by induced gynogenesis, interspecific hybridization, microsatellite genotyping and multilocus DNA fingerprinting. Zool Sci 19:565–575

    Article  PubMed  Google Scholar 

  • Morishima K, Nakamura-Shiokawa Y, Bando E, Li YJ, Boron A, Khan MMR, Arai K (2008a) Cryptic clonal lineages and genetic diversity in the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) inferred from nuclear and mitochondrial DNA analyses. Genetica 132:59–69

    Article  Google Scholar 

  • Morishima K, Yoshikawa H, Arai K (2008b) Meiotic hybridogenesis in triploid Misgurnus loach derived from a clonal lineage. Heredity 100:581–586

    Article  CAS  PubMed  Google Scholar 

  • Nygren A, Nilsson B, Jahnke M (1972) Cytological studies in Atlantic salmon from Canada, in hybrids between Atlantic salmon from Canada and Sweden and in hybrids between Atlantic salmon and sea trout. Hereditas 70:295–306

    Article  CAS  PubMed  Google Scholar 

  • Piferrer F, Beaumont A, Falguiere J-C, Flajshans M, Haffray P, Colombo L (2009) Polyploid fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture 293:125–156

    Article  Google Scholar 

  • Shimizu Y, Shibata N, Yamashita M (1997) Spermiogenesis without proceeding meiosis in the hybrid medaka between Oryzias latipes and O. curvinotus. J Exp Zool 279:102–112

    Article  Google Scholar 

  • Suzuki R, Nakanishi T, Oshiro T (1985) Survival, growth and sterility of induced triploids in the cyprinid loach Misgurnus anguillicaudatus. Bull Jpn Soc Sci Fish 51:889–894

    Article  Google Scholar 

  • Wu C (1990) Retrospects and prospects of fish genetics and breeding research in China. Aquaculture 86:111–125

    Article  Google Scholar 

  • Yoshikawa H, Morishima K, Fujimoto T, Saito T, Kobayashi T, Yamaha E, Arai K (2009) Chromosome doubling in early spermatogonia produces diploid spermatozoa in a natural clonal fish. Biol Reprod 80:973–979

    Article  CAS  PubMed  Google Scholar 

  • Zhang Q, Arai K (1996) Flow cytometry for DNA contents of somatic cells and spermatozoa in the progeny of natural tetraploid loach. Fish Sci 62:870–877

    CAS  Google Scholar 

  • Zhang Q, Arai K (1999) Aberrant meioses and viable aneuploidy progeny of induced triploid (Misgurnus anguillicaudatus) when crossed to natural tetraploids. Aquaculture 175:63–76

    Article  Google Scholar 

  • Zhang Q, Arai K, Yamashita M (1998) Cytogenetic mechanisms for triploid and haploid egg formation in the triploid loach Misgurnus anguillicaudatus. J Exp Zool 281:608–619

    Article  Google Scholar 

  • Zhang Q, Hanada K, Arai K (2002) Aberrant meioses in diploid and triploid progeny of gynogenetic diploids produced from eggs of natural tetraploid loach. Folia Zool 51:165–176

    Google Scholar 

  • Zhao Y, Saito T, Pšenička M, Fujimoto T, Arai K (2014) Comparison of spermatozoa parameters, fine structures, and energy-related factors among tetraploid, hyper-tetraploid, and hyper-triploid loaches (Misgurnus anguillicaudatus). J Exp Zool A Ecol Genet Physiol 321A:198–206

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported in part by KAKENHI Grant-in-Aid for Scientific Research (B) (No. 21380114) from the Japan Society of the Promotion of Science (JSPS) to K.A. and Grants-in-Aid from the Natural Science Foundation of China (No. 31272650) and the Natural Science Foundation of Liaoning Province (No. 201102019) to Y.J.L.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Katsutoshi Arai.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, YJ., Gao, YC., Zhou, H. et al. Meiotic chromosome configurations in triploid progeny from reciprocal crosses between wild-type diploid and natural tetraploid loach Misgurnus anguillicaudatus in China. Genetica 143, 555–562 (2015). https://doi.org/10.1007/s10709-015-9853-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10709-015-9853-2

Keywords

Navigation