Abstract
Peroxiredoxin1(Prx1), also known as natural killer enhancing factor A (NKEF-A), is a crucial antioxidant involving in various cellular activities and immune response against bacterial and viral infection in fish. In the present study, a full-length Prx1 cDNA sequence (TfPrx1) was firstly cloned from roughskin sculpin (Trachidermus fasciatus), which was composed of 1044 bp nucleotides encoding a peptide of 199 amino acids with a molecular weight of 22.35 kDa and a theoretical pI of 6.42, respectively. The predicted peptide was a typical 2-cys Prx containing two conserved characteristic motifs 43FYPLDFTFVCPTEI56 and 170GEVCPA175 with the two conserved peroxidatic and resolving cysteine residuals forming disulfide bond. Quantitative real-time PCR analysis showed that TfPrx1 was ubiquitously expressed in all tested tissues with the highest expression in the intestine. It could be significantly induced following LPS injection and heavy metal exposure. Recombinant TfPrx1 (rTfPrx1) displayed insulin disulfide reduction and ROS-scavenging activity in a concentration-dependent manner, and further exhibited DNA and cytoprotective effects under oxidative stress. These results suggested that TfPrx1 protein may play an important role in fish immune protection from oxidative damage.
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Funding
This work was supported by the Natural Science Foundation of Shandong Province, China (ZR2016CP10), the Weihai Science Technology Bureau (1070413421509), the State Oceanic Administration of China (201105011), and the Ministry of Agriculture of the People’s Republic of China (1070413701307).
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Yingying Liu: methodology, investigation, software, data curation, and writing—original draft. Haiyan Cong: investigation, software, and data curation. Caihong Bi: investigation, methodology, and software. Haidong Zha: investigation and methodology. Shanshan Yu: investigation and methodology. Lihua Zhao: investigation. Qian Zhu: conceptualization, supervision, and writing—review and editing.
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All experimental procedures of this study were in line with the animal ethics committee of Shandong University and the Department of Central Lab, Weihai Municipal Hospital (No. 2023002).
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Liu, Y., Cong, H., Bi, C. et al. Molecular characterization and functional analysis of peroxiredoxin 1 (Prx1) from roughskin sculpin (Trachidermus fasciatus). Fish Physiol Biochem 50, 513–526 (2024). https://doi.org/10.1007/s10695-023-01281-7
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DOI: https://doi.org/10.1007/s10695-023-01281-7