Abstract
In a study on the anti-nutritional effect of dietary fiber, it was noticed that a high-pectin diet (PEC diet) caused growth retardation, hepatic cholestasis, steatosis, fibrosis, and enteritis accompanied by decreased glycocholic acid (GCA) in Pelteobagrus fulvidraco. This study was conducted to investigate the potential alleviating effects of supplementation with GCA. A PEC diet and a diet supplemented with 0.6 g kg−1 GCA based on the PEC diet (named the GCA diet) were formulated and randomly fed to juvenile Pelteobagrus fulvidraco. Compared to fish that were fed the PEC diet for 7 days, the GCA content in liver increased significantly in fish fed the GCA diet, the incidence of abnormal liver color, gallbladder somatic index (GBSI), total bile acid concentration in serum and liver, and the expression of arnesoid X receptor gene (fxr) upregulated and genes involved in bile acid (BA) synthesis and uptake in liver decreased significantly. After 56 days, the SGR, the expression of fxr and genes involved in BA synthesis and transportation in the liver, the serum content of total bilirubin, total protein, and globulin were significantly higher, while the hepatosomatic index, GBSI, liver lipid and collagen content, and the incidence of distal intestine tissue damage were lower in fish fed the GCA diet than in those fed the PEC diet. These results suggested that GCA improved growth performance and alleviated hepatic cholestasis and tissue damage to the liver and intestine induced by a high-pectin diet, which might occur through activating FXR.
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The datasets generated during and/or analyzed during the current study are not publicly available but are available from the corresponding author on reasonable request.
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Acknowledgements
We sincerely thank Qin Ni, Xinyue Li, Yunhe Meng, and Zijing Meng for their assistance in gene expression and serum biochemical analysis in this study.
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This work was supported by the Provincial Science and Technology Innovative Program for Carbon Peak and Carbon Neutrality of Jiangsu of China (BE2022422), Jiangsu Modern Agricultural (fresh fish) Industrial Technology System (JATS (2022) 408), and PAPD of Soochow University.
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Shibin Yao: investigation, validation, formal analysis, data curation, writing original draft. Shengjie Ren: investigation, data curation, validation, formal analysis. Chunfang Cai: supervision, writing — review and editing, conceptualization, project administration, funding acquisition. Xiamin Cao: conceptualization. Ye Shi: investigation. Pin Wu and Yuantu Ye: supervision, conceptualization.
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Yao, S., Ren, S., Cai, C. et al. Glycocholic acid supplementation improved growth performance and alleviated tissue damage in the liver and intestine in Pelteobagrus fulvidraco fed a high-pectin diet. Fish Physiol Biochem 50, 41–57 (2024). https://doi.org/10.1007/s10695-022-01148-3
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DOI: https://doi.org/10.1007/s10695-022-01148-3