Abstract
Bile acids (BAs) are a class of cholesterol-derived amphipathic molecules approved as new animal feed additives. However, the functional researches mainly focused on BAs mixture, and the influence of the individual BA on fishes was still limited. In the present study, Nile tilapia were fed basal diet with three levels of sodium taurocholate at 0 mg/kg (CON), 300 mg/kg (TCAL), and 600 mg/kg (TCAH) for 8 weeks. The results indicated that addition of sodium taurocholate did not significantly influence the growth performance. Instead, TCAH group had higher cholesterol accumulation with liver fibrosis. In TCAH group, the level of nuclear factor E2-related factor 2 (nrf2) signaling-associated oxidative stress factors significantly increased in the liver. Additionally, fish in TCAH group had the highest expression level of genes encoding endoplasmic reticulum (ER) stress and inflammatory cytokines in the liver. In conclusion, 300 mg/kg of sodium taurocholate did not significantly influence the growth performance of fish, while 600 mg/kg of sodium taurocholate markedly induced cholesterol accumulation and liver injury, suggesting that the application of taurocholic acid in aquafeed should be re-evaluated.
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This work was financed by the National Natural Science Foundation of China (grant number 31972798).
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Mei-Ling Zhang, Fei-Fei Ding, and Miao Li conceived the study and designed the experiments. Miao Li and Fei-Fei Ding carried out the experiments and analyzed the data. Fei-Fei Ding and Mei-Ling Zhang wrote and revised the manuscript. Fei-Fei Ding, Miao Li, Tong Wang, Nan-Nan Zhou, Fang Qiao, Zhen-Yu Du and Mei-Ling Zhang contributed to the material preparation, methodology, and supervision. All authors read and approved the final manuscript.
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The growth experiment of the fish was conducted strictly in Guidance of the Care and Use of Laboratory Animals in China. The procedures used for fish was approved by the Committee on the Ethics of Animal Experiments of East China Normal University (ECNU20201002).
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Ding, FF., Li, M., Wang, T. et al. Influence of dietary sodium taurocholate on the growth performance and liver health of Nile tilapia (Oreochromis niloticus). Fish Physiol Biochem 50, 319–330 (2024). https://doi.org/10.1007/s10695-022-01116-x
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DOI: https://doi.org/10.1007/s10695-022-01116-x