Abstract
In European aquaculture, Eurasian perch, Perca fluviatilis L., is perceived as one of the most highly valuable freshwater fish species and a strong candidate for the development of freshwater aquaculture. In the pursuit of improving the quality of reproduction in this domesticated species, investigating the hormones mediating the final oocyte maturation (FOM) is therefore indispensable. But, the exact nature of the maturation-inducing hormone (MIH) in Eurasian perch is unknown. To further validate the existence of a maturation-inducing activity behind potential hormonal candidates in this species, we in vitro tested a group of nine hormones: cortisol (Co), 11-deoxycortisol (11-D), corticosterone (coS), 11-deoxycorticosterone (DOC), 17α,20βdihydroxy-4-pregnen-3-one (DHP) and 17α,20β,21 trihydroxy-4-pregnen-3-one (THP), prostaglandin E2 (PGE2), estradiol-17β (E2) and testosterone (T), in their ability to trigger FOM advancement and the production of sex steroids potentially involved in FOM. Using mature female perch, two in vitro experiments were conducted with oocytes at the start of the FOM. The follicles were incubated for 62 h in Cortland media with and without human chorionic gonadotropin (hCG). By the end of the incubation, only DHP and THP triggered the full advancement in FOM even at low doses with the effect of DHP being in vivo validated. However, the de novo productions of E2 and DHP were not shown to be regulated by either of the MIH candidates. Progestagens are hence more credible candidates as MIH than corticosteroids in Eurasian perch. Our in vitro study also revealed that both PGE2 and DHP are strongly associated with ovulation and that PGE2 might have slightly contributed to such DHP activity.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Leila El Mohajer: conceptualization, culture experiments, in vivo experiment, analysis, writing
Rose Bulteau: analysis
Céline Chevalier: in vivo experiment
Sirine Selmi: culture experiments
Pascal Fontaine: conceptualization
Sylvain Milla: conceptualization, culture experiments, in vivo experiment, writing
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All experimental manipulations were carried out in agreement with the European and French national legislations on animal welfare after evaluation and approval of the experimental project (protocol number: APAFIS15307-2018053110404570 and 23871-2020013017452359) by the local ethic committee in France (Name: CELMEA).
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El Mohajer, L., Bulteau, R., Chevalier, C. et al. In vitro follicle culture shows that progestagens are the maturation-inducing hormones (MIH) and possible regulators of the ovulation-mediating hormone PGE2 in female Eurasian perch Perca fluviatilis. Fish Physiol Biochem 47, 881–894 (2021). https://doi.org/10.1007/s10695-021-00946-5
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DOI: https://doi.org/10.1007/s10695-021-00946-5