Abstract
According to the importance of vitamin B6 (pyridoxine) as a water-soluble vitamin on the physiological conditions of aquatic animals, the present study aimed to investigate effects of different concentrations of this vitamin in recycle system culture water on the Nile tilapia (Oreochromis niloticus). Treatments including 0 (control), 10, 20, 30, and 40 mg L−1 vitamin B6 were adjusted in triplicate recirculating systems. Each of the experimental tanks (100 L) was stocked 15 fingerling Nile tilapia during 60-day experimental period. According to the findings, weight gain in treatments of 30 and 40 mg L−1 pyridoxine was significantly higher than the other treatments while blood cortisol hormone in the treatment of 40 mg L−1 was significantly highest among the treatments. In addition, mid-intestine trypsin activity in the treatment of 40 mg L−1 was significantly higher than the other treatments. The histological analysis of the intestine showed that the number of mucus-secreting cells significantly decreased in treatments of 30 and 40 mg L−1. Our findings here suggest that pyridoxine can possibly be absorbed by the Nile tilapia’s body through culture water and it seems 20–30 mg L−1 pyridoxine in the culture water is the optimal concentration for the Nile tilapia juveniles in recycle system culture.
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Acknowledgments
The authors wish to thank the Fisheries Department of Natural Resources Faculty of the University of Tehran for providing experimental facilities. Also, we would like to sincerely thank Prof. Rafiee for their technical assistance.
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Current research was funded by the University of Tehran under grant number 26713.
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The trial protocol was approved by the Ethics Committee for the Animal Research, University of Tehran; none of the fish suffered starvation, trauma, or electrical shock and all the fish were completely anesthetized before tissue sampling.
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Javanmardi, S., Rezaei Tavabe, K., Rosentrater, K.A. et al. Effects of different levels of vitamin B6 in tank water on the Nile tilapia (Oreochromis niloticus): growth performance, blood biochemical parameters, intestine and liver histology, and intestinal enzyme activity. Fish Physiol Biochem 46, 1909–1920 (2020). https://doi.org/10.1007/s10695-020-00840-6
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DOI: https://doi.org/10.1007/s10695-020-00840-6