Abstract
To understand the physiological response of estuarine fish to acidification, barramundi (Lates calcarifer) juveniles were exposed to acidified seawater in experimental conditions. The molecular response of barramundi to acidification stress was assessed by RNA-seq analysis. A total of 2188 genes were identified as differential expression genes. The gene ontology classification system and Kyoto Encyclopedia of Genes and Genomes database analysis showed that acidification caused differential expressions of genes and pathways in the gills of barramundi. Acidification had a great influence on the signal transduction pathway in cell process. Furthermore, we detected that numerous unigenes involved in the pathways associated with lipid metabolism, carbohydrate metabolism, amino acid metabolism, glycan biosynthesis and metabolism specific and non-specific immunity were changed. This study indicates that the physiological responses in barramundi especially the immune system and energy allocation correspond to the variation of environmental pH. This study reveals the necessity for assessment of the potential of estuarine fishes to cope with acidification of the environment and the need to develop strategies for fish conservation in coastal areas.
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Acknowledgements
The authors are grateful for the technical support from Shanghai Majorbio Bio-Pharm Technology Co., Ltd.
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This research was financially supported by the South China Sea Fisheries Research Institute, CAFS (2017ZD01, 2018ZD01), Guangxi Innovation-driven Development Projects (Guike AA18242031), Central Public-interest Scientific Institution Basal Research Fund, and Key Research and Development Plan of Hainan Province (ZDYF2017036, ZDYF2018096).
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The experimental procedure was complied with the standards of Institutional Animal Care and Use Committee guidelines of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences. All experiments were conducted in line with the principles and guidelines for the care and use of live fish and the guidelines for animal experimentation approved by the Animal Experimental Council of South China Sea Fisheries Research Institute (Approved No. 2017ZD01).
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Ma, Z., Zheng, X., Fu, Z. et al. Transcriptional analysis reveals physiological response to acute acidification stress of barramundi Lates calcarifer (Bloch) in coastal areas. Fish Physiol Biochem 46, 1729–1741 (2020). https://doi.org/10.1007/s10695-020-00824-6
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DOI: https://doi.org/10.1007/s10695-020-00824-6