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Melanins as biomarkers of ovarian follicular atresia in the catfish Heteropneustes fossilis: biochemical and histochemical characterization, seasonal variation and hormone effects

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Abstract

Follicular atresia is a common feature of the vertebrate ovary that occurs at different stages of folliculogenesis and ovarian regression. It has physiological significance to maintain homeostasis and control fecundity, and ensure removal of post-ovulatory follicular remnants for preparing the ovary for the next cycle. Pigments appear late in the atretic process as indigestible waste formed out of the degradation of the oocytes, follicle wall and granulocytes. In the present study, pigment accumulation was demonstrated by Schmorl’s and Perls’ staining methods in the atretic ovarian follicles of Heteropneustes fossilis during follicular development and regression. Melanins were characterized spectrophotometrically for the first time in fish ovary. The predominant form is eumelanin, followed by pheomelanin and alkali-soluble melanin. Melanins showed significant seasonal variations with levels low in gonad resting phase, increasing to the peak in the post-spawning phase. The concentration of melanins increased time-dependently in post-ovulated ovary after human chorionic gonadotropin treatment. In the spawning phase, in vitro incubation of ovary slices with estradiol-17β or dexamethasone for 8 or 16 h decreased both eumelanin and pheomelanin levels time-dependently. The alkali-soluble melanin showed a significant decrease only in the dexamethasone group at 16 h. The results show that melanin assay can be used as a biomarker of follicular atresia in fish ovary, natural or induced by environmental toxicants.

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Acknowledgments

RK is a recipient of Rajiv Gandhi National Fellowship of UGC, New Delhi, which is gratefully acknowledged.

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  1. Department of Zoology, Centre of Advanced Study, Banaras Hindu University, Varanasi, 221005, India

    Ravi Kumar & Keerikkattil P. Joy

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  1. Ravi Kumar
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  2. Keerikkattil P. Joy
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Correspondence to Keerikkattil P. Joy.

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Kumar, R., Joy, K.P. Melanins as biomarkers of ovarian follicular atresia in the catfish Heteropneustes fossilis: biochemical and histochemical characterization, seasonal variation and hormone effects. Fish Physiol Biochem 41, 761–772 (2015). https://doi.org/10.1007/s10695-015-0044-y

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  • DOI: https://doi.org/10.1007/s10695-015-0044-y

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