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Production and characterization of recombinant Manchurian trout thyrotropin

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Abstract

Thyrotropin (thyroid-stimulating hormone, TSH), a heterodimeric glycoprotein hormone produced in the pituitary, stimulates the thyroid gland and release of thyroid hormones. In contrast to a well-known efficacy of recombinant mammalian TSHs, there is no report about the production of teleost recombinant TSH and its biological activity. In this study, we report the production of a single-chain recombinant TSH (mtTSH) of Manchurian trout (Brachymystax lenok), by baculovirus in silkworm (Bombyx mori) larvae. The mtTSH was produced in silkworm larvae and characterized as a form of N-linked glycosylation. The cAMP signaling system in transiently transfected COS-7 cells revealed that the mtTSH was recognized by their cognate receptors, salmon TSHα and TSHβ receptors, but not LH receptor. The thyrotropic potency of the mtTSH was examined by rainbow trout basibranchial tissues containing thyroid follicles. The height of follicle epithelial cells was significantly increased by treatments of mtTSH in vivo and in vitro. In conclusion, the present study suggests that the mtTSH produced by baculovirus–silkworm larvae is a biologically active recombinant TSH.

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Acknowledgments

We thank Dr. Yoshitaka Nagahama, National Institute for Basic Biology, Okazaki, Aichi, Japan, for supplying the amago salmon LH and TSH receptor vectors. We also thank the personnel of Salmon Research Center, Yang–Yang, Gangwon-do, Korea, for providing experimental fish. This work was supported by Grants-in-Aid from the JSPS, Japan, for Japan-Korea Basic Scientific Cooperation, the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST; 2012R1A1A2044506), and Gangneung-Wonju National University (2011-0190).

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Authors and Affiliations

  1. Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung, 210-702, Republic of Korea

    Jihye Shin, Mi Ae Kim & Young Chang Sohn

  2. Department of Life Science, International Christian University, Tokyo, 181-8585, Japan

    Makito Kobayashi

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  1. Jihye Shin
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  2. Mi Ae Kim
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  3. Makito Kobayashi
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  4. Young Chang Sohn
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Correspondence to Young Chang Sohn.

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Shin, J., Kim, M.A., Kobayashi, M. et al. Production and characterization of recombinant Manchurian trout thyrotropin. Fish Physiol Biochem 39, 1353–1363 (2013). https://doi.org/10.1007/s10695-013-9789-3

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  • DOI: https://doi.org/10.1007/s10695-013-9789-3

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