Skip to main content
SpringerLink
Log in

Catalasic activity in fish liver: improvement of the UV to visible analytic method

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

Antioxidative defenses and more especially catalasic activity (CAT) are studied in a large range of scientific research thematics. In environmental sciences, the problematic of oxidative stress is of great interest as pollutants can induce perturbations of redox homeostasis. Consequently, changes in antioxidative defenses levels in fish tissues and particularly in liver are used as potential biomarkers of pollution. In most studies, the CAT was assayed by following during 5 min the consumption of H2O2 in cytosolic buffered extracts at 240 nm (UV-method). This study proposed a development of this method in the visible, using permanganate and a 525-nm detection, which was more accurate, sensitive, and rapid. Moreover, the hepatic CAT of six different fish species [a cyclidae (Nimbochromis linni), 3 cyprinidae (Brachydanio rerio, Rutilus rutilus, Cyprinus carpio), an anguillidae (Anguilla anguilla), and a percidae (Perca fluviatilus)] was evaluated with the two protocols (UV- and KMnO4-method). The results but also the thermal optimum of the reaction and the interest of CAT as biomarker in ecotoxicology were discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Ahmad I, Hamid T, Fatima M, Chand HS, Jain SK, Altran M, Raisuddin S (2000) Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure. Biochem Biophys Acta 15(23):37–48

    Google Scholar 

  • Ahmad I, Pacheco M, Santos MA (2004) Enzymatic and non enzymatic antioxidants as an adaptation to phagocyte-induced damage in Anguilla anguilla L. following in situ harbour water exposure. Ecotoxicol Environ Saf 57:290–302

    Article  CAS  PubMed  Google Scholar 

  • Ahmad I, Oliveira M, Pacheco M, Santos MA (2005) Anguilla anguilla L. oxidative stress biomarkers responses to copper exposure with or without b-naphthoflavone pre-exposure. Chemosphere 61:267–275

    Article  CAS  PubMed  Google Scholar 

  • Ahmad I, Pacheco M, Santos MA (2006) Anguilla anguilla L. oxidative stress biomarkers: an in situ study of freshwater wetland ecosystem (Pateira de Fermentelos, Portugal). Chemosphere 65(6):952–962

    Article  CAS  PubMed  Google Scholar 

  • Almroth BC, Sturve J, Berglund A, Förlin L (2005) Oxidative damage in eelpout (Zoarces viviparous), measured as protein carbonyl and TBARS, as biomarkers. Aquat Toxicol 73:171–180

    Article  CAS  PubMed  Google Scholar 

  • Aruoma OL (1998) Free radicals, oxidative stress and antioxidants in human health and disease. J Am Oil Chem 75:199–212

    Article  CAS  Google Scholar 

  • Babo S, Vasseur P (1992) In vitro effects of thiram on liver antioxidant enzyme activities of rainbow trout (Oncorhynchus mykiss). Aquat Toxicol 22(1):61–68

    Article  CAS  Google Scholar 

  • Beliaeff B, Burgeot T (2002) Integrated biomarker response: a useful tool for ecological risk assessment. Environ Toxicol Chem 21:1316–1322

    Article  CAS  PubMed  Google Scholar 

  • Benedetti M, Fattorini D, Martuccio G, Nigro M, Regoli F (2009) Interactions between trace metals (Cu, Hg, Ni, Pb) and 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin in the Antarctic fish Trematomus bernacchii: oxidative effects on biotransformation pathway. Environ Toxicol Chem 28:818–825

    Article  CAS  PubMed  Google Scholar 

  • Beutler E (1975) Red cell metabolism: a manual of biochemical methods. Grune and Straton, New York

    Google Scholar 

  • Bhatia S, Shukla R, Madhu SV, Gambhir JK, Prabhu KM (2003) Antioxidant status, lipid peroxidation and NO end products in patients of type 2 diabetes mellitus with nephropathy. Clin Biochem 36:557–562

    Article  CAS  PubMed  Google Scholar 

  • Biagianti-Risbourg S, Paris-Palacios S, Mouneyrac C, Amiard-Triquet C (2012) Pollution acclimation, adaptation, resistance and tolerance in ecotoxicology. Encyclopedia of aquatic ecotoxicology In: Férard JF and Blaise C (eds) Springer, pp 135–146

  • Bradford MM (1976) A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Broeg K, Westernhagen HV, Zander S, Körting W, Koehler A (2005) The “bioeffect assessment index”—a concept for the quantification of effects of marine pollution by an integrated biomarker approach. Mar Pollut Bull 50:495–503

    Article  CAS  PubMed  Google Scholar 

  • Cavalheiro de Menezes C, Braga da Fonseca M, Pretto A, Dos Santos Miron D, Santi A (2011) Oxidative parameters of Rhamdia quelen in response to commercial herbicide containing clomazone and recovery pattern. Pestic Biochem Physiol 100(2):145–150

    Article  CAS  Google Scholar 

  • Celi P (2011) Biomarkers of oxidative stress in ruminant medicine. Immunopharm Immunotox 33(2):233–240

    Article  Google Scholar 

  • Cohen G, Dembiec D, Marcus J (1970) Measurement of catalase activity in tissue extracts. Anal Biochem 34:30–38

    Article  CAS  PubMed  Google Scholar 

  • Crestani M, Menezes C, Glusczak L, Miron DS, Spanevello R, Silveira A, Gonçalves FF, Zanella R, Loro VL (2007) Effects of clomazone herbicide on biochemical and histological aspects of silver catfish (Rhamdia quelen) and recovery pattern. Chemosphere 67:2305–2311

    Article  CAS  PubMed  Google Scholar 

  • Di Matteo V, Esposito E (2003) Biochemical and therapeutic effects of antioxidants in the treatment of Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. Curr Drug Targets CNS Neurol Disord 2:95–107

    Article  PubMed  Google Scholar 

  • Galloway TS, Brown RJ, Browne MA, Dissanayake A, Lowe D, Jones MB, Depledge MH (2004) A multibiomarker approach to environmental assessment. Environ Sci Technol 38:1723–1731

    Article  CAS  PubMed  Google Scholar 

  • Gerber M, Boutron-Ruault MC, Hercberg S, Riboli E, Scalbert A, Siess MH (2002) Food and cancer: state of the art about the protective effect of fruits and vegetables. Bull Cancer 89:293–312

    PubMed  Google Scholar 

  • Gorbi S, Regoli F (2003) Total oxyradical scavenging capacity as an index of susceptibility to oxidative stress in marine organisms. Comments Toxicol 9:303–322

    Article  CAS  Google Scholar 

  • Góth L (1991) A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 196:143–152

    Article  PubMed  Google Scholar 

  • Góth L, Németh H, Mészáros I (1984) Clinical study of the determination of serum catalase enzyme activity. Hung Sci Inst 57:7–12

    Google Scholar 

  • Johansson J, Hakan Borg LA (1988) A spectrophotometric method for determination of catalase activity in small tissue samples. Anal Biochem 174:331–336

    Article  CAS  PubMed  Google Scholar 

  • Keramati V, Jamili S, Ramin M (2010) Effect of diazinon on catalase antioxidant enzyme activity in liver tissue of Rutilus rutilus. J Fish Aquat Sci 5(5):368–376

    Article  CAS  Google Scholar 

  • Krishnaiah D, Sarbatly R, Nithyanandam R (2011) A review of the antioxidant potential of medicinal plant species. Food Bioprod Proc 89(3):217–233

    Article  CAS  Google Scholar 

  • Lefer DJ, Granger DN (2000) Oxidative stress and cardiac disease. Am J Med 109:315–323

    Article  CAS  PubMed  Google Scholar 

  • Leighton F, Poole B, Beaufay H, Baudhuin P, Coffey JW, Fowler S, De Duve C (1968) The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with Triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions. J Cell Biol 37:482–513

    Article  CAS  PubMed  Google Scholar 

  • Mao YX, Zheng H, Guo XQ, Chen R, Zheng WY (2002) Studies on the fluorometric method for determining activity of catalase and its application to marine biosamples. Chem J Chinese Univ 23(10):18–67

    Google Scholar 

  • Masuoka N, Wakimoto M, Ubuka T, Nakano T (1996) Spectrophotometric determination of hydrogen peroxide: catalase activity and rates of hydrogen peroxide removal by erythrocytes. Clin Chim Acta 254:101–112

    Google Scholar 

  • Modesto KA, Martinez CBR (2010) Roundup® causes oxidative stress in liver and inhibits acetylcholinesterase in muscle and brain of the fish Prochilodus lineatus. Chemosphere 78:294–299

    Article  CAS  PubMed  Google Scholar 

  • Montavon P, Kukic KR, Bortlik K (2007) A simple method to measure effective catalase activities: optimization, validation, and application in green coffee. Anal Biochem 360(2):207–215

    Article  CAS  PubMed  Google Scholar 

  • Moraes BS, Loro VL, Glusczak L, Pretto A, Menezes C, Marchezan E, Machado SO (2007) Effects of four rice herbicides on some metabolic and toxicology parameters of teleost fish (Leporinus obtusidens). Chemosphere 68:1597–1601

    Article  CAS  PubMed  Google Scholar 

  • Mueller S, Riedel HD, Stremmel W (1997a) Determination of catalase activity at physiological hydrogen peroxide concentrations. Anal Biochem 245:55–60

    Article  CAS  PubMed  Google Scholar 

  • Mueller S, Riedel HD, Stremmel W (1997b) Direct evidence for catalase as the predominant H2O2-removing enzyme in human erythrocytes. Blood 15:4973–4978

    Google Scholar 

  • Paris-Palacios S, Biagianti-Risbourg S, Vernet G (2000a) Biochemical and (ultra)structural hepatic perturbations of Brachydanio rerio (Teleostei, Cyprinidae) exposed to two sublethal concentrations of copper sulfate. Aquat Toxicol 50(1–2):109–124

    Article  CAS  PubMed  Google Scholar 

  • Paris-Palacios S, Biagianti-Risbourg S, Fouley A, Vernet G (2000b) Metallothioneins in liver of Rutilus rutilus exposed to Cu2+. Analysis by metal summation, SH determination and spectrofluorimetry. Comp Biochem Physio C 126(2):113–122

    CAS  Google Scholar 

  • Paris-Palacios S, Biagianti-Risbourg S, Vernet G (2003) Metallothionein induction related to hepatic structural perturbations and antioxidative defences in roach (Rutilus rutilus) exposed to the fungicide procymidone. Biomarkers 8(2):128–141

    Article  CAS  PubMed  Google Scholar 

  • Peuchant E, Brun J, Rigalleau V, Dubourg L, Thomas M, Daniel J (2004) Oxidative and antioxidative status in pregnant women with either gestational or type 1 diabetes. Clin Biochem 37:293–298

    Article  CAS  PubMed  Google Scholar 

  • Piva F, Francesco Ciaprini F, Onorati F, Benedetti M, Fattorini D, Ausili A, Regoli F (2011) Assessing sediment hazard through a weight of evidence approach with bioindicator organisms: a practical model to elaborate data from sediment chemistry, bioavailability, biomarkers and ecotoxicological bioassays. Chemosphere 83(4):475–485

    Article  CAS  PubMed  Google Scholar 

  • Regoli F, Pellegrini D, Winston GW, Gorbi S, Giuliani S, Virno-Lamberti C, Bompadre S (2002) Application of biomarkers for assessing the biological impact of dredged materials in the Mediterranean: the relationship between antioxidant responses and susceptibility to oxidative stress in the red mullet (Mullus barbatus). Mar Pollut Bull 44:912–922

    Article  CAS  PubMed  Google Scholar 

  • Regoli F, Frenzilli G, Bocchetti R, Annarumma F, Scancelli V, Fattorini D, Nigro M (2004) Time-course variations of oxyradical metabolism, DNA integrity, and lysosomal stability in mussels, Mytilus galloprovincialis, during a field translocation experiment. Aquat Toxicol 68:167–178

    Article  CAS  PubMed  Google Scholar 

  • Sayeed I, Parvez S, Pandey S, Bin-Hafeez B, Rizwanul H, Raisuddin S (2003) Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus bloch. Ecotoxicol Environ Saf 56:295–301

    Article  CAS  PubMed  Google Scholar 

  • Scandalious JG (1997) Molecular biology of the antioxidant defence genes encoding catalases and superoxide dismutases in maize. In: Hatzios KK (ed) Regulation of enzymatic systems detoxifying xenobiotics in plants. Kluver Academic, Dordrecht, pp 97–108

    Chapter  Google Scholar 

  • Shahidi F, Janitha PK, Wanasundara PD (1992) Phenolic antioxidants. Crit Rev Food Sci Nutr 32:67–103

    Article  CAS  PubMed  Google Scholar 

  • Slaughter MR, O’Brien PJ (2000) Fully-automated spectrophotometric method for measurement of antioxidant activity of catalase. Clin Biochem 33(7):525–534

    Article  CAS  PubMed  Google Scholar 

  • Smith MA, Rottkamp CA, Nunomura A, Raina AK, Perry G (2000) Oxidative stress in Alzheimer’s disease. Biochem Biophys Acta 1502:139–144

    Article  CAS  PubMed  Google Scholar 

  • Sreejayan N, Rao M (1996) Free radical scavenging activity of Curcuminoids. Drug Res 46:169–171

    CAS  Google Scholar 

  • Steer P, Milligard J, Sarabi DM, Wessby B, Kahan T (2002) Cardiac and vascular structure and function are related to lipid peroxidation and metabolism. Lipids 37:231–236

    Article  CAS  PubMed  Google Scholar 

  • Sullivan JB (1992) Cyrogenics, oxidizers, reducing agents, and explosives. In: Sullivan JB, Krieger GR (eds) Hazardous materials toxicology. Clin. Principles Environ health. Baltimore, Williams & Wilkins, pp 1192–1201

  • Toni C, Menezes CC, Loro VL, Clasen BE, Cattaneo R, Santi A, Pretto A, Zanella R, Leitemperger J (2010) Oxidative stress biomarkers in Cyprinus carpio exposed to commercial herbicide bispyribac-sodium. J Appl Toxicol 30:590–595

    Article  CAS  PubMed  Google Scholar 

  • Uchida K (2000) Role of reactive aldehyde in cardiovascular diseases. Free Radical Biol Med 28:1685–1696

    Article  CAS  Google Scholar 

  • Van der Oost R, Beyer J, Vermeulen NPE (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ Toxicol Pharmacol 13:57–149

    Article  PubMed  Google Scholar 

  • Van Lente F, Pepoy M (1990) Coupled-enzyme determination of catalase activity in erythrocytes. Clin Chem 36:1339–1343

    PubMed  Google Scholar 

  • Whaley-Connell A, McCullough PA, Sowers JR (2011) The role of oxidative stress in the metabolic syndrome. Rev Cardiovasc Med 12(1):21–29

    PubMed  Google Scholar 

  • Wheeler CR, Salzman JA, Elsayed NB, Omaye ST, Korte DW (1990) Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Anal Biochem 184:193–199

    Article  CAS  PubMed  Google Scholar 

  • Winston GW, DiGiulio RT (1991) Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat Toxicol 19(2):137–161

    Article  CAS  Google Scholar 

  • Yildirim NC, Benzer F, Danabas D (2011) Evaluation of environmental pollution at Munzur river of tunceli applying oxidative stress biomarkers in Capoeta trutta (Heckel, 1843). J Plant Sci 21(1):66–71

    CAS  Google Scholar 

Download references

Acknowledgments

The present study was financially supported by the French National Research Agency through the program CIEL (ANR CESA005 2008).

Author information

Authors and Affiliations

  1. Laboratoire d’Ecologie-Ecotoxicologie, Faculté des Sciences, EA4689 Interaction Animal-Environnement (IAE), Université de Reims Champagne-Ardenne, Moulin de la Housse, B.P. 1039-51687, Reims Cedex 2, France

    Séverine Paris-Palacios, Laurence Delahaut, Alexis Carreras & Sylvie Biagianti-Risbourg

  2. Unité de Recherche Animal et Fonctionnalités des Produits Animaux (UR AFPA), Université Henri Poincaré de Nancy 1, Muséum Aquarium de Nancy, 34 rue Sainte Catherine, 54000, Nancy, France

    Marielle Thomas

Authors
  1. Séverine Paris-Palacios
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Laurence Delahaut
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alexis Carreras
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marielle Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sylvie Biagianti-Risbourg
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Séverine Paris-Palacios.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Paris-Palacios, S., Delahaut, L., Carreras, A. et al. Catalasic activity in fish liver: improvement of the UV to visible analytic method. Fish Physiol Biochem 39, 957–966 (2013). https://doi.org/10.1007/s10695-012-9754-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10695-012-9754-6

Keywords

Search

Navigation