Abstract
The role of gonadotropin (GTH) in the reproduction of the Japanese flounder, Paralichthys olivaceus, was studied by assessing the changes in the apparent activity of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the pituitary gland during gonadal maturation by immunohistochemical analyses. Corresponding changes in plasma levels of testosterone (T), estradiol-17β (E2), and 17α,20β-dihydroxy-4–pregnen-3-one (DHP) were also studied. Reared fish at the early spawning to termination stages were sampled from May to August and wild fish at the previtellogenic to termination stages were caught at 3- to 4-week intervals between April and September offshore from the northern mainland of Japan by gill nets. The gonadosomatic index of the reared fish decreased from the early spawning stage to the termination stage, while that of the wild fish increased significantly from the previtellogenic stage to the early spawning stage and decreased thereafter. In the reared fish, the immunostaining intensities of FSH and LH were high during the spawning period, accompanied by high plasma levels of T, E2, and DHP. In the wild fish, the immunostaining intensities of FSH and LH were low during the previtellogenic stage but increased during the maturing and spawning stages. These results indicate that both FSH and LH are likely associated with oocyte maturation in the Japanese flounder.
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Acknowledgements
We are grateful to Dr. Masato Aritaki and the staff at the Miyako Station, National Center for Stock Enhancement, Fisheries Research Agency, Japan, for assisting with the experiments at the station. We would also like to thank Ms. Hiroe Aoki and Ms. Mio Matsuki, School of Fisheries Sciences, Kitasato University, Japan, for their help in collecting samples for this study.
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Pham, K.X., Amano, M., Kurita, Y. et al. Changes in the immunostaining intensities of follicle-stimulating hormone and luteinizing hormone during ovarian maturation in the female Japanese flounder. Fish Physiol Biochem 34, 357–365 (2008). https://doi.org/10.1007/s10695-007-9195-9
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DOI: https://doi.org/10.1007/s10695-007-9195-9