Abstract
Synthetic hybrids is one of the ways to heterosis application in rapeseed, which can effectively solve the problems of the promotion of extra-early-maturing rapeseed varieties in high altitude areas. The flowering period, the yield and the outcrossing rate of the restorer lines, and the yield of their F1s crossed with the sterile line are crucial factors for breeding synthetic hybrids. In this study, one excellent extra-early-maturing Brassica napus sterile line and 28 restorer lines were used as plant materials. Three excellent restorer lines (2036, 137, and 52) were selected by investigating the flowering period, the genetic differences and the yield of each restorer line, and the yield of their hybrid F1 crossed with the sterile line. DNA fingerprinting was performed with SSR molecular markers. The three restorer lines had outcrossing rates from high to low as follows: 137 (59.00%) > 2036 (46.90%) > 52 (30.55%). In addition, the pollen tube germination of each restorer line was observed, and their hybridization and self-compatibility were explored to explain the rates of outcrossing. Collectively, our results suggest that the proportion of the three restorer lines was 137 > 2036 > 52 when using these three extra-early-maturing and sterile rapeseed lines to prepare synthetic hybrids.
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Funding
This research was supported by the Earmarked Fund for China Agriculture Research System (CRAS-12), the Qinghai Natural Science Foundation Program-innovation team (2022-ZJ-902), and the Qinghai Innovation Platform Construction Project (2022-ZJ-Y01; 2022-ZJ-Y13).
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DD conceived and designed the study. LZ and KL performed the experiments, analyzed the data and wrote the manuscript. LZ, KL and YJ modified the article. All authors read and approved the manuscript.
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Zan, L., Li, K., Jia, Y. et al. Screening and evaluation of three restorer lines used to create synthetic hybrids of extra-early-maturing Brassica napus. Euphytica 219, 45 (2023). https://doi.org/10.1007/s10681-023-03175-4
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DOI: https://doi.org/10.1007/s10681-023-03175-4