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Stacking of Pup1 QTL for low soil phosphorus tolerance and bacterial blight resistance genes in the background of APMS6B, the maintainer line of rice hybrid DRRH-3

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Abstract

Phosphorus (P) is one of the macronutrients essential for plant growth and development. Rice (Oryza sativa L.) is sensitive to P starvation and its deficiency influences many key plant functions, resulting in crop yield penalties. Although the hybrid rice segment is well-known for its yield heterosis, P deficiency and bacterial leaf blight diseases are evident limitations. APMS6B, the female parent of DRRH-3 is susceptible to low P and bacterial blight disease. In the present study, the improvement of APMS6B to P starvation and resistance to bacterial leaf blight (BB) was carried out using the marker-assisted backcross breeding approach. Kasalath (+Pup1 QTL) was used as a donor, and a promising IL (ATR 594-1) at BC1F4 generation was identified with 81.15% of recurrent parent genome recovery. Concurrently, this IL was intercrossed with GU-2 (+Xa21 and Xa38). Intercross F1s (ICF1) hybridity was confirmed through foreground selection having maximum RPGR (88.29%) and was selfed to produce ICF2. The resultant progenies were phenotyped for BB using Xoo inoculum (IX-020), simultaneously genotyped with gene-specific functional SSR markers forXa21 and Xa38. The identified BB-resistant plants were subjected to foreground selection for Pup1. Four promising ICF3 plants (BP-10-1, BP-10-3, BP-10-5, and BP-10-15 with Xa21, Xa38, and Pup1) along with parents and checks were screened both in low P plot (< 2 kg P2O5 ha−1) as well as in normal plot (> 25 kg P2O5 ha−1) during dry and wet seasons 2018. The field evaluation identified four promising intercrossed lines with better root growth in the primary root length of extracted zone and root volume. In addition, fewer reductions in grain yield (39.10%) under P starvation and less susceptibility indices values (< 1) for BB were observed. These lines may be exploited in the CMS conversion and development of climate-resilient, biotic and abiotic stress-tolerant rice hybrids.

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Acknowledgements

Authors are grateful to the Indian Council of Agriculture Research (ICAR), ICAR-Indian Institute of Rice Research (ICAR-IIRR), and Govt. of India for their financial support and facilities for research.

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Authors and Affiliations

  1. Crop Improvement Section, ICAR-Indian Institute of Rice Research, Hyderabad, India

    N. Madhusudan, P. Beulah, V. Jaldhani, P. Nagaraju, Y. Manasa, R. M. Sundaram, G. S. Laha, M. S. Anantha, Kalyani M. Barbadikar, C. Gireesh, A. S. HariPrasad, M. Sheshu Madhav, R. Gobinath, K. B. Kemparaju, C. N. Neeraja, P. Brajendra, M. D. Tuti, R. Mahender Kumar & P. Senguttuvel

  2. Molecular Physiology, Heinrich Heine University, 40225, Düsseldorf, Germany

    A. Yugandhar

  3. Professor Jaya Shankar Telangana State Agricultural University (PJTSAU), Hyderabad, India

    K. V. Radha Krishna

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  1. N. Madhusudan
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  2. P. Beulah
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  3. V. Jaldhani
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  5. Y. Manasa
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  7. G. S. Laha
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  21. P. Senguttuvel
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Contributions

MN and SP Conceived, planned and designed the study. MN, BeP, VJ, NP and MY Conducted the experiments. MN and SP Analyzed the data and wrote the manuscript. AMS, KKB, KMB, LGS, GC, GR, ASH, BrP, TMD and MKR Critically edited the manuscript. SP and SRM Helped in the coordination of the study and edited the final draft of the manuscript.

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Correspondence to R. M. Sundaram or P. Senguttuvel.

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Madhusudan, N., Beulah, P., Jaldhani, V. et al. Stacking of Pup1 QTL for low soil phosphorus tolerance and bacterial blight resistance genes in the background of APMS6B, the maintainer line of rice hybrid DRRH-3. Euphytica 218, 37 (2022). https://doi.org/10.1007/s10681-022-02987-0

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