Abstract
Rice growth and productivity are greatly affected by cold stress, which is likely to become more of a hindrance for high and stable rice yields. To identify cold tolerance at the booting and flowering stages in rice, a recombinant inbred line was developed by crossing a cold-tolerant japonica cultivated variety, Jileng1, with a cold-sensitive indica cultivated variety, Milyang23. The seed setting rate (SST) of the parents and RIL population were investigated under different temperature environments, and the SST and cold stress tolerance index under natural low temperature were used to evaluate cold tolerance and quantitative trait locus (QTL) mapping. Fifteen QTLs were detected on chromosomes 1, 2, 3, 5, 7, 11 and 12, with log-likelihood values ranging from 2.64 to 4.76. These QTLs account for 3.34% to 12.02% of the phenotypic variance explained. Three QTLs, qCtb1, qCtb5 and qCtb12, were repeatedly detected in different conditions, and they were considered stably expressed QTLs. qCtb5 was localized to chromosome 5 marker between CMB0526.3 and ID5014265. In this interval or nearby, many cold-resistance QTLs have been identified in previous studies, so qCtb5 is considered a major cold-tolerance QTL. Thirteen QTLs with environmental interactions were also detected, and QTLs detected in single environment were all found to be involved in environmental interactions. These results show that environmental interactions have a significant effect on cold tolerance in rice. Stable expression of major QTLs will help to fine mapping cold-tolerance genes and provide gene resources to cultivate cold-tolerance rice varieties.
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All data included in this study are available upon request by contact with the corresponding author.
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Acknowledgements
This work was supported by the National Key Research and Development Program of China (2016YFD0100101,2016YFD0100301), the National Natural Science Foundation of China (31671664), the National Natural Sciences Foundation (31670326), Technology Innovation and Application Development Program in Chongqing (cstc2019jscx-msxmX0353), CAAS Science and Technology Innovation Program, National Infrastructure for Crop Germplasm Resources (NICGR2018-01), Protective Program of Crop Germplasm of China (2018NWB036-01, 2018NWB036-122).
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LZ conducted field work, generated phenotypic data, preformed data analysis and wrote the manuscript; JT generated phenotypic data and genotypic data; DC preformed the genotyping of the mapping population; CT helped for field work; XM helped for field work; XA helped for field work; BH helped for field work; GC helped for field work; ZZ designed the research and manuscript revision; H-JK designed the research and manuscript revision; LH conceived the experiment, guided experiments and manuscript revision. All authors read and approved the final version.
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Zhang, L., Tang, J., Cui, D. et al. Identification of QTLs for cold tolerance at the booting and flowering stages in rice (Oryza sativa L.). Euphytica 217, 214 (2021). https://doi.org/10.1007/s10681-021-02898-6
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DOI: https://doi.org/10.1007/s10681-021-02898-6