Skip to main content
SpringerLink
Log in

Improved marker-assisted selection efficiency of multi-resistance in doubled haploid rice plants

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

During rice cultivation, brown planthopper (BPH, Nilaparvata lugens Stal.) and white-backed planthopper (WBPH, Sogatella furcifera) infestations cause decreased yield and rice quality. In this study, we attempted to develop multi-resistance plants and increase the marker-assisted selection (MAS) efficiency. The frequency of callus induction in 95 F1 doubled haploid lines (DHLs) derived from JSNDH13 (BPH resistance)/CNDH32 (WBPH resistance) ranged from 3.8 to 15.2 %. The plant regeneration rates varied from 2.9 to 39.3 %. Selected 75 DH plants were used to compare the results of WBPH bioassay and DNA marker reaction. The DHL bioassay showed continuous gene distribution and resistance score ranged from 0.5 to 9.0 (mean, 4.2). The broad-sense heritability for WBPH resistance was 51.7 %. The DHLs from the cross of JSNDH13/CNDH32 were segregated into 39 resistant (R):36 susceptible (S) to WBPH, i.e., 1 RR:SS. For the bioassay and MAS, RM11669 with WBPH resistance was selected. Genetically, the use of resistant cultivars, MAS, and DH breeding has proven to be environment friendly, efficient, and economical. This study confirmed the genetic basis for MAS to improve WBPH resistance in DHLs of WBPH/BPH resistant rice plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

WBPH:

Whitebacked planthopper

BPH:

Brown planthopper

MAS:

Marker assisted selection

DHLs:

Doubled haploid lines

JSNDH13:

Junam//Samkang/Nagdong Doubled Haploid-13

CNDH32:

Cheongcheong/Nagdong Doubled Haploid-32

References

  • Akhtar S, Bhat MA, Wani A, Bhat KA, Chalkoo S, Mir MR, Wani SA (2010) Marker assisted selection in rice. J Phytol 2:66–81

    Google Scholar 

  • Chen QF, Wang CL, Lu YM, Shen M, Afza RA, Duren MV, Brunner H (2001) Anther culture in connection with induced mutations for rice improvement. Euphytica 120:401–408

    Article  Google Scholar 

  • Collard BCY, Jahufer MZZ, Brouwer JB, Pang ECK (2005) An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: the basic concepts. Euphytica 142:169–196

    Article  CAS  Google Scholar 

  • Deen R, Ramesh K, Gautam SK, Rao YK, Lakshmi VJ, Viraktamath BC, Brar DS, Ram T (2005) Identification of new gene for BPH resistance introgressed from O. rufipogon. Rice Genet Newsl 25:70–71

    Google Scholar 

  • Germanà MA (2011) Anther culture for haploid and doubled haploid production. Plant Cell Tissue Organ Cult 104:283–300

    Article  Google Scholar 

  • Goncharova YK (2013) Selective elimination of alleles in rice anther culture. Russ J Genet 49:170–177

    Article  CAS  Google Scholar 

  • He T, Yang Y, Tu SB, Yu MQ, Li XF (2006) Selection of interspecific hybrids for anther culture of indica rice. Euphatica 86:271–277

    Google Scholar 

  • Kang WH, Luitel BP, Adhikari PB, Shrestha SL (2012) Morphological characterization of anther derived plants in minipaprika (Capsicum annuum L.). Korean J Breed Sci 44:450–461

    Article  Google Scholar 

  • Kim KM, Nam WI, Kwon YS, Sohn JK (2004) Development of doubled-haploid population and construction of genetic map using SSR markers in rice. J Plant Biotechnol 31:179–184

    Article  Google Scholar 

  • Kim TH, Kim KM, Manigbas NL, Yi GH, Sohn JK (2013) Identification of quantitative trait loci (QTL) for resistance to white-backed planthopper (Sogatella furcifera) in rice with background of Milyang 46 (Cheongcheong). Philipp J Crop Sci 38:30–36

    Google Scholar 

  • Kwon YS, Sohn JK (2000) Varietal difference and inheritance of plant regenerability in anther culture of rice. Korean J Plant Tissue Cult 27:163–167

    Google Scholar 

  • Lee JK, Lee SY, Kang HJ, Lee YT, Noh MS, Shin HT, Lee SY, Cho SY, Kim CH, Shin MS, Kim BK, Kim YD, Lee KS, Ko JK, Ha KY, Kim HS, Joung JI (1996) An anther-derived good quality and high yielding rice variety “Hwashinbyeo”. Korean J Breed Sci 28:480

    CAS  Google Scholar 

  • Lee JH, Yeo US, Kwak DY, Park DS, Kim CS, Shin MS, Oh BG, Ku YC, Sohn JK (2007) Identification of DNA marker for brown planthopper resistance gene Bph1 in rice (Oryza sativa L.). Korean J Breed Sci 39:172–177

    Google Scholar 

  • Moon HP, Kim HY, Cho SY, Kang KH, Choi HC, Choi IS, Shin YS, Kim KW, Choi YG, Park RK, Kim YS (1994) An anther-derived high quality rice variety, “Hwajoongbyeo”. Korean J Breed Sci 26:451

    Google Scholar 

  • Oh BG, Lim SJ, Yang SJ, Hwang HG, Yi GH, Yeo US, Park NB, Kwak DY, Kim SC, Kim HY, Oh YJ, Jun BT (1997) An anther-derived high eating quality and high yielding rice variety with resistance to bacterial leaf blight “Hwasambyeo”. Korean J Breed Sci 2:496

    Google Scholar 

  • Seo JP, Yang SJ, Jeung JU, Pamplona A, Kim JJ, Lee JH, Hong HC, Yang CI, Kim YG, Jena KK (2011) Development of elite breeding lines conferring Bph18 gene derived resistance to brown planthopper (BPH) by marker assisted selection and genome-wide background analysis in japonica rice(Oryza sativa L.). Field Crop Res 120:215–222

    Article  Google Scholar 

  • Shin MS, Kim WJ, Shin WC, Park HS, Seo CS, Choi IB, Ha KY, Kang HJ, Ko JK (2011) Effect of brown planthopper resistance gene, Bph18 to yield components in rice. Korean J Breed Sci 43:56–61

    Google Scholar 

  • Sogawa K, Jiang HU, Zeng LJ, Qian Q, Zeng D (2005) Resistance performance to whitebacked planthopper in different phenotypes of japonica/indica doubled haploid rice lines. Rice Sci 12:133–136

    Google Scholar 

  • Sohn JK, Yi GH, Oh BG, Lim SJ (1995) Variation of some agronomic traits in anther-derived rice plants. Korean J Breed Sci 27:404–408

    Google Scholar 

  • Sugiura N, Tsuji T, Fujii K, Kato T, Saka N, Touyama T, Hayano SY, Izawa T (2004) Molecular marker-assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.). Breed Res 6:143–148

    Article  Google Scholar 

  • Sun L, Su C, Wang C, Zhai H, Wan J (2005) Mapping of a major resistant gene to the brown planthopper in the rice cultivar Rathu Heenati. Breed Sci 55:391–396

    Article  CAS  Google Scholar 

  • Yi G, Won Y, Ko J, Park H, Cho J, Oh B, Yang S, Kim S, Nam MH (2003) Effected of cold shock pretreatment and carbohydrate sources on anther culture of rice. J Plant Biotechnol 30:369–373

    Article  Google Scholar 

  • Yun SH (2014) Studies on the optimum screening for improved WBPH-associated QTL analysis in rice. Master Thesis/Dissertation. Kyungpook National University, South Korea

Download references

Acknowledgments

This study was carried out with the support of the Cooperative Research Program for Agriculture & Technology Development (Project No. PJ0080622014), Rural Development Administration, Republic of Korea.

Author information

Authors and Affiliations

  1. Department of Farm Management, College of Agriculture and Life Science, Kyungpook National University, Gunwi-gun, Gyeongbuk, 716-821, Korea

    Gihwan Yi

  2. Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, 702-701, Korea

    Hyun-Suk Lee & Kyung-Min Kim

Authors
  1. Gihwan Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyun-Suk Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kyung-Min Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kyung-Min Kim.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yi, G., Lee, HS. & Kim, KM. Improved marker-assisted selection efficiency of multi-resistance in doubled haploid rice plants. Euphytica 203, 421–428 (2015). https://doi.org/10.1007/s10681-014-1303-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-014-1303-1

Keywords

Search

Navigation