Skip to main content
SpringerLink
Log in

Development and identification of a introgression line with strong drought resistance at seedling stage derived from Oryza sativa L. mating with Oryza rufipogon Griff

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Drought stress is severely damaging during seedling stage of rice (Oryza sativa L.), which can lead to significant yield reductions. Dongxiang common wild rice (Oryza rufipogon Griff., hereafter referred to as DXWR), with strong drought resistance, could be a favorable genetic resource to improve the drought resistance of cultivated rice. Xieqingzao B (O. sativa L. ssp. indica, hereafter referred to as XB) is a representative maintainer line in hybrid rice breeding system in China. By using DXWR as donor parent, XB as recurrent parent, through continuous selfing, backcrossing and strict drought-resistant screening, we developed a strong and stable drought-resistant introgression line IL395 (BC5F10), whose ability of drought resistance was significantly increased than that of the recurrent parent XB at the seedling stage. Meanwhile, no significant differences existed among other major agronomic traits under normal condition, except for plant height. Physiological assessment revealed that IL395 exhibited a significant increase in levels of free proline and soluble sugars, which was associated with drought resistance. Whole genome marker analyses identified genomic segments of DXWR linking with RM171 and RM590 (chr. 10) and RM235 (chr. 12) that require further analysis as possible sources of drought resistance trait. These results suggest that DXWR could be a favorable genetic resource to improve the drought resistance of cultivated rice, and the IL395 might be a useful resource for excavating the drought-resistant genes from DXWR.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Atwell BJ, Wang H, Scafaro AP (2014) Could abiotic stress tolerance in wild relatives of rice be used to improve Oryza sativa? Plant Sci 216:48–58

    Article  Google Scholar 

  • Bouman BAM, Peng S, Castaòeda AR, Visperas RM (2005) Yield and water use of irrigated tropical aerobic rice systems. Agric Water Manag 74:87–105

    Article  Google Scholar 

  • Chaves MM, Oliveira MM (2004) Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. J Exp Bot 55:2365–2384

    Article  PubMed  CAS  Google Scholar 

  • Chen XR, Yang KS, Fu JR, Zhu CL, Peng XS, He XP, He HH (2008) Identification and genetic analysis of fertility restoration ability in Dongxiang wild rice (Oryza rufipogon). Rice Sci 15:21–28

    Article  Google Scholar 

  • Ding XP, Li XK, Xiong LZ (2011) Evaluation of near-isogenic lines for drought resistance QTL and fine mapping of a locus affecting flag leaf width, spikelet number, and root volume in rice. Theor Appl Genet 123:815–826

    Article  PubMed  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Firon N, Nepi M, Pacini E (2012) Water status and associated processes mark critical stages in pollen development and functioning. Ann Bot 109:1201–1214

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Gu JF, Yin XY, Struik PC, Stomph TJ, Wang HQ (2012) Using chromosome introgression lines to map quantitative trait loci for photosynthesis parameters in rice (Oryza sativa L.) leaves under drought and well-watered field conditions. J Exp Bot 63:455–469

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Hammer K (2004) Resolving the challenge posed by agrobiodiversity and plant genetic resources—an attempt. J Agric Rural Dev Tropics Subtrop 76:184 Kassel University Press, Beiheft

    Google Scholar 

  • Hemamalini GS, Shashidhar HE, Hittalmani S (2000) Molecular marker assisted tagging of morphological and physiological traits under two contrasting moisture regimes at peak vegetative stage in rice (Oryza sativa L.). Euphytica 112:69–78

    Article  CAS  Google Scholar 

  • Hu BL, Fu XQ, Zhang T, Wan Y, Li X, Huang YH, Dai LF, Luo XD, Xie JK (2011) Genetic analysis on characteristics to measure drought resistance using Dongxiang wild rice (Oryza rufupogon Griff.) and its derived backcross inbred lines population at seedling stage. Agric Sci China 10:1653–1664

    Article  Google Scholar 

  • Huang DH, Qiu YF, Zhang YX, Huang F, Meng JR, Wei SM, Li RB, Chen BS (2013) Fine mapping and characterization of BPH27, a brown planthopper resistance gene from wild rice (Oryza rufipogon Griff.). Theor Appl Genet 126:219–229

    Article  PubMed  CAS  Google Scholar 

  • Igarashi Y, Yoshiba Y, Sanada Y, Yamaguchi-Shinozaki K, Wada K, Shinozaki K (1997) Characterization of the gene for deltal-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and salt tolerance in Oryza sativa L. Plant Mol Biol 33:857–865

    Article  PubMed  CAS  Google Scholar 

  • Khoury CK, Greene S, Wiersema J, Maxted N, Jarvis A, Struik PC (2013) An inventory of crop wild relatives of the United States. Crop Sci 53:1496–1508

    Article  Google Scholar 

  • Khush GS (2005) What it will take to feed 5.0 billion rice consumers in 2030. Plant Mol Biol 59:1–6

    Article  PubMed  CAS  Google Scholar 

  • Koh S, Lee SC, Kim MK, Koh JH, Lee S, An G, Choe S, Kim SR (2007) T-DNA tagged knockout mutation of rice OsGSK1, an orthologue of Arabidopsis BIN2, with enhanced tolerance to various abiotic stresses. Plant Mol Biol 65:453–466

    Article  PubMed  CAS  Google Scholar 

  • Kovach MJ, Sweeney MT, McCouch SR (2007) New insights into the history of rice domestication. Trends Genet 23:578–587

    Article  PubMed  CAS  Google Scholar 

  • Lanceras JC, Pantuwan G, Jongdee B, Toojinda T (2004) Quantitative trait loci associated with drought tolerance at reproductive stage in rice. Plant Physiol 135:384–399

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Li Z, Zhu Y (1988) Rice male sterile cytoplasm and fertility restoration. Hybrid Rice. International Rice Research Institute, Manila, pp 85–102

    Google Scholar 

  • Li ZC, Mu P, Li CP, Zhang HL, Li ZK, Gao YM, Wang XK (2005) QTL mapping of root traits in a doubled haploid population from a cross between upland and lowland japonica rice in three environments. Theor Appl Genet 110:1244–1252

    Article  PubMed  CAS  Google Scholar 

  • Liu FX, Sun CQ, Tan LB, Fu YC, Li DJ, Wang XK (2003) Identification of QTL for cold tolerance at booting and flowering stage in Jiangxi Dongxiang wild rice. Chin Sci Bull 48:1864–1867

    Google Scholar 

  • Liu JX, Liao DQ, Oane R, Estenor L, Yang XE, Li ZC, Bennett J (2006) Genetic variation in the sensitivity of anther dehiscence to drought stress in rice. Field Crop Res 97:87–100

    Article  Google Scholar 

  • Luo XD, Dai LF, Cao JF, Jian SR, Chen YL, Hu BL, Xie JK (2012) Identification and molecular cytology analysis of cold tolerance introgression lines derived from Oryza sativa L. mating with O. rufipogon Griff. Euphytica 187:461–469

    Article  Google Scholar 

  • Mani S, Van De Cotte B, Van Montagu M, Verbruggen N (2002) Altered levels of proline dehydrogenase cause hypersensitivity to proline and its analogs in Arabidopsis. Plant Physiol 128:73–83

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • McCouch SR, Teytelman L, Xu YB, Lobos KB, Clare K, Walton M, Fu BY, Maghirang R, Li ZK, Xing YZ, Zhang QF, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res 9:199–207

    Article  PubMed  CAS  Google Scholar 

  • Passioura JB (2007) The drought environment: physical, biological and agricultural perspectives. J Exp Bot 58:113–117

    Article  PubMed  CAS  Google Scholar 

  • Penna S (2003) Building stress tolerance through over-producing trehalose in transgenic plants. Trends Plant Sci 8:355–357

    Article  PubMed  CAS  Google Scholar 

  • Plucknett DL, Smith NJH, Williams JT, Anishetty NM (1987) Gene banks and the world’s food. Princeton University Press, Princeton

    Google Scholar 

  • Qiu YF, Guo JP, Jing SL, Zhu LL, He GC (2012) Development and characterization of japonica rice lines carrying the brown planthopper-resistance genes BPH12 and BPH6. Theor Appl Genet 124:485–494

    Article  PubMed  CAS  Google Scholar 

  • Ravi M, Geethanjali S, Sameeyafarheen F, Maheswaran M (2003) Molecular marker based genetic diversity analysis in rice (Oryza sativa L.) using RAPD and SSR markers. Euphytica 133:243–252

    Article  CAS  Google Scholar 

  • Robin S, Pathan MS, Courtois B, Lafitte R, Carandang S, Lanceras S, Amante M, Nguyen HT, Li Z (2003) Mapping osmotic adjustment in an advanced back-cross inbred population of rice. Theor Appl Genet 107:1288–1296

    Article  PubMed  CAS  Google Scholar 

  • Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. Plant Mol Biol Manual 6:1–10

    Google Scholar 

  • Sakai H, Itoh T (2010) Massive gene losses in Asian cultivated rice unveiled by comparative genome analysis. BMC Genom 11:121

    Article  Google Scholar 

  • Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58:221–227

    Article  PubMed  CAS  Google Scholar 

  • Smale M, Bellon MR, Jarvis D, Sthapit B (2004) Economic concepts for designing policies to conserve crop genetic resources on farms. Genet Resour Crop Evol 51:121–135

    Article  Google Scholar 

  • Takeshi F, Xiong LZ (2013) Genetic mechanisms conferring adaptation to submergence and drought in rice: simple or complex? Curr Opin Plant Biol 16:196–204

    Article  Google Scholar 

  • Tian F, Li DJ, Fu Q, Zhu ZF, Fu YC, Wang XK, Sun CQ (2006) Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (Oryza sativa L.) background and characterization of introgressed segments associated with yield-related traits. Theor Appl Genet 112:570–580

    Article  PubMed  CAS  Google Scholar 

  • Troll W, Lindsley J (1955) A photometric method for the determination of proline. J Biol Chem 215:655–660

    PubMed  CAS  Google Scholar 

  • Vikram P, Swamy BP, Dixit S, Ahmed HU, Teresa Sta Cruz M, Singh AK, Kumar A (2011) qDTY1.1, a major QTL for rice grain yield under reproductive-stage drought stress with a consistent effect in multiple elite genetic backgrounds. BMC Genet 12:89

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Xie JK, Kong XL, Bao JS, Wan Y (2004) Recent advances in molecular mapping and cloning of useful genes from wild rice and their application in breeding. Hereditas (Beijing). 26:115–121

    CAS  Google Scholar 

  • Xie JK, Hu BL, Wan Y, Zhang T, Li X, Liu RL, Huang YH, Dai LF, Luo XD (2010) Comparison of the drought resistance characters at seedling stage between Dongxiang common wild rice (Oryza rufipogon Griff.) and cultivars (Oryza sativa L.). Acta Ecol Sin 30:1665–1674

    Google Scholar 

  • Xiong LM, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14:165–183

    Article  Google Scholar 

  • Yue B, Xue WY, Xiong LZ, Yu XQ, Luo LJ, Cui KH, Jin DM, Xing YZ, Zhang QF (2006) Genetic basis of drought resistance at reproductive stage in rice: separation of drought tolerance from drought avoidance. Genetics 172:1213–1228

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhang XS, Zhou X, Fu YC, Zhen S, Wang XK, Sun CQ (2006) Identification of a drought tolerant introgression line derived from Dongxiang common wild rice (O. rufipogon Griff.). Plant Mol Biol 62:247–259

    Article  PubMed  CAS  Google Scholar 

  • Zhang LD, Yu SW, Zuo KJ, Luo LJ, Tang KX (2012) Identification of gene modules associated with drought response in rice by network-based analysis. PLoS One 7:e33748

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Zheng BS, Yang L, Zhang WP, Mao CZ, Wu YR, Yi KK, Liu FY, Wu P (2003) Mapping QTLs and candidate genes for rice root traits under different water-supply conditions and comparative analysis across three populations. Theor Appl Genet 107:1505–1515

    Article  PubMed  CAS  Google Scholar 

  • Zhou YH, Lam HM, Zhang JH (2007) Inhibition of photosynthesis and energy dissipation induced by water and high light stresses in rice. J Exp Bot 58:1207–1217

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to Ms. Feifei Sun (Technische Universität Dresden, Germany) for her critical reading of the manuscript in this work. This research was partially supported by the National Natural Science Foundation of China (31201191, 30960189, 31360327), the Natural Science Foundation of Jiangxi Province, China (20131522040024, 2010GZN0109), the Special Fund for Agro-scientific Research in the Public Interest (201103007), the Major Projects in Jiangxi Province (20114ABF03101), and the Key Projects of Jiangxi Education Department (KJLD12059, GTT11386).

Author information

Authors and Affiliations

  1. College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, China

    Fantao Zhang, Fenglei Cui, Liangxing Zhang, Xiufang Wen, Xiangdong Luo, Yi Zhou, June Zhang & Jiankun Xie

  2. Biotechnology Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China

    Xia Li & Yong Wan

Authors
  1. Fantao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fenglei Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liangxing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiufang Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiangdong Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yi Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xia Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yong Wan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. June Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jiankun Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Fantao Zhang or Jiankun Xie.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, F., Cui, F., Zhang, L. et al. Development and identification of a introgression line with strong drought resistance at seedling stage derived from Oryza sativa L. mating with Oryza rufipogon Griff. Euphytica 200, 1–7 (2014). https://doi.org/10.1007/s10681-014-1121-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-014-1121-5

Keywords

Search

Navigation