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Selective genotyping to identify late blight resistance genes in an accession of the tomato wild species Solanum pimpinellifolium

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Abstract

Late blight (LB), caused by the oomycete Phytophtohra infestans, is one of the most destructive diseases of tomato (Solanum lycopersicum) and other Solanaceae species. Current disease control and prevention strategies are not sufficient to control the disease in tomato. Recent germplasm screening experiments led to the identification of a new source of resistance (PI 270443) in the tomato wild species S. pimpinellifolium. This study was conducted to identify genomic regions associated with LB resistance in this accession. A large F2 population (n = 986) derived from a cross between PI 270443 and a LB-susceptible tomato breeding line (NCEBR-2) was screened for LB resistance using a highly aggressive isolate of P. infestans. Twenty-five of the most resistant and 29 of the most susceptible, but surviving F2 individuals were identified based on disease evaluations conducted in the F2 and F3 progeny populations. The selected individuals were genotyped with 153 DNA markers located across the 12 tomato chromosomes. A selective genotyping approach led to the identification of two genomic regions on tomato chromosomes 1 and 10 associated with LB resistance in PI 270443. Identification of two genomic regions associated with resistance was consistent with a previous estimate of the number of LB resistance genes in this accession. Research is currently underway to fine map the two resistance genes and incorporate them into new tomato breeding lines and hybrid cultivars.

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Acknowledgments

The authors graciously thank Dr. Matthew Kinkade for his help with development of SSR markers and all other Penn State staff and undergraduate students who helped with disease screening and data collection. The authors also are thankful to Dr. Randolph Gardner for providing seeds of control genotypes NC84173, NCEBR-2, NC63 EB, NC870, NC03220 and cultivar New Yorker, to the USDA PGRU for providing the original seed of PI 270443, and to Dr. Seong-Hwan Kim of the Pennsylvania Department of Agriculture for providing original isolates of P. infestans. This research was supported in part by Agricultural Research Funds administered by the Pennsylvania Department of Agriculture, the Pennsylvania Vegetable Marketing and Research Program, and the College of Agricultural Sciences at the Pennsylvania State University.

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  1. Heather L. Merk

    Present address: Department of Horticulture and Crop Sciences, OARDC, Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA

  2. Hamid Ashrafi

    Present address: Department of Plant Sciences, Seed Biotechnology Center, University of California, Davis, CA, 95616, USA

Authors and Affiliations

  1. Department of Horticulture and the Intercollege Graduate Degree Program in Genetics, The Pennsylvania State University, University Park, PA, 16802, USA

    Heather L. Merk, Hamid Ashrafi & Majid R. Foolad

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  1. Heather L. Merk
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Correspondence to Majid R. Foolad.

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Merk, H.L., Ashrafi, H. & Foolad, M.R. Selective genotyping to identify late blight resistance genes in an accession of the tomato wild species Solanum pimpinellifolium . Euphytica 187, 63–75 (2012). https://doi.org/10.1007/s10681-012-0729-6

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