Abstract
The resistance genes Lr9, Lr24, Lr25, Lr29, Lr35 and Lr37, which were not previously utilised in Hungary, have been incorporated into four Martonvásár winter wheat cultivars using marker-assisted selection with PCR-based markers. In the course of a backcross programme, the genes were transferred into Martonvásár wheat varieties and various BC generations were produced. Work aimed at pyramiding resistance genes is currently underway in Martonvásár, and plants containing the gene combinations Lr9 + Lr24, Lr9 + Lr25 and Lr9 + Lr29 are now available. From the BC2F4 generation of the ‘Mv Emma’*3/’R.L.6010’ combination (‘R.L.6010’ is the donor of the Lr9 gene) 287 lines were tested for leaf rust resistance in an artificially inoculated nursery. A co-dominant primer combination was designed to identify both resistant and susceptible offsprings. The results of resistance tests and molecular marker detection agreed in most cases. Designated leaf rust resistance genes were identified with molecular markers in wheat varieties and breeding lines. The Lr26 and Lr34 resistance genes occur frequently in the Martonvásár gene pool, and the presence of the Lr37 gene has also been detected in a number of Hungarian genotypes.
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Ayliffe M, Singh R, Lagudah E (2008) Durable resistance to wheat stem rust needed. Curr Opin Plant Biol 11:187–192. doi:10.1016/j.pbi.2008.02.001
Barabás Z, Matuz J (1983) A levélrozsda és a lisztharmat epidémia, illetve különféle rezisztencia típusok befolyása őszi búza genotípusok termésére (Yield of winter wheat genotypes as affected by leaf rust and powdery mildew epidemics as well as by the type of resistance). Novenytermeles 32:193–207
Bayles RA, Flath K, Hovmøller MS et al (2000) Breakdown of the Yr17 resistance to yellow rust of wheat in northern Europe. Agronomie 20:805–811. doi:10.1051/agro:2000176
Brown-Guerdira GL, Singh S, Fritz AK (2003) Performance and mapping of leaf rust resistance to wheat from Triticum timopheevii subsp. armeniacum. Phytopathol 93:784–789. doi:10.1094/PHYTO.2003.93.7.784
Browning JA, Frey KJ (1969) Multiline cultivars as a means of disease control. Annu Rev Phytopathol 7:355–382. doi:10.1146/annurev.py.07.090169.002035
Cherukuri DP, Gupta PK, Charpe A et al (2005) Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr28 in wheat. Euphytica 143:19–26. doi:10.1007/s10681-005-1680-6
Chu CC, Hill RD, Brule-Babel AL (1990) High frequency of pollen embryoid formation and plant regeneration in Triticum aestivum L. on monosaccharide containing media. Plant Sci 66:255–262. doi:10.1016/0168-9452(90)90211-6
Danna CH, Sacco F, Ingala LR et al (2002) Cloning and mapping of genes involved in wheat-leaf rust interaction through gene-expression analysis using chromosome-deleted near-isogenic wheat lines. Theor Appl Genet 105:972–979. doi:10.1007/s00122-002-0990-5
Dedryver F, Jubier MF, Thouvenin J et al (1996) Molecular markers linked to the leaf rust resistance gene Lr24 in different wheat cultivars. Genome 39:830–835
Dhaliwal AS, Mares DJ, Marshall DR (1987) Effect of 1B/1R chromosome translocation on milling and quality characteristics of bread wheats. Cereal Chem 64:72–76
Dyck PL (1994) Genetics of resistance to leaf rust and stem rust on wheat. Annu Wheat Newsl 40:63–64
Feuillet C, Travella S, Stein N et al (2003) Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat Triticum aestivum L genome. Proc Natl Acad Sci USA 100:15253–15258. doi:10.1073/pnas.2435133100
Gál M, Vida G, Uhrin A et al (2007) Incorporation of leaf rust resistance genes into winter wheat genotypes using marker-assisted selection. Acta Agronomica Hung 55:149–156. doi:10.1556/AAgr.55.2007.2.2
Gold J, Harder D, Townley-Smith F et al. (2002) Development of molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding lines. http://www.ejbiotechnology.cl/content/vol2/issue1/full/1/index.html. Accessed 28 Nov 2008
Gupta SK, Charpe A, Koul S et al (2005) Development and validation of molecular markers linked to an Aegilops umbellulata-derived leaf-rust-resistance gene, Lr9, for marker-assisted selection in bread wheat. Genome 48:823–830
Helguera M, Khan IA, Dubcovsky J (2000) Development of PCR markers for the wheat leaf rust resistance gene Lr47. Theor Appl Genet 100:1137–1143. doi:10.1007/s001220051397
Helguera M, Khan IA, Kolmer J et al (2003) PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci 43:1839–1847
Helguera M, Vanzetti L, Soria M et al (2005) PCR markers for Triticum speltoides leaf rust resistance gene Lr51 and their use to develop isogenic hard red spring wheat lines. Crop Sci 45:728–734
Herrera-Foessel SA, Djurle A, Yuen J et al (2007) Identification and molecular characterization of leaf rust resistance gene Lr14a in durum wheat. Plant Dis 92:469–473. doi:10.1094/PDIS-92-3-0469
Hiebert C, Thomas J, McCallum B (2005) Locating the broad-spectrum wheat leaf rust resistance gene Lr52 by a new cytogenetic method. Theor Appl Genet 111:1453–1457. doi:10.1007/s00122-005-1978-8
Hiebert CW, Thomas JB, Somers DJ et al (2007) Microsatellite mapping of adult-plant resistance gene Lr22a in wheat. Theor Appl Genet 115:877–884. doi:10.1007/s00122-007-0604-3
Huang L, Gill BS (2001) An RGA-like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops tauschii and wheat. Theor Appl Genet 103:1007–1013. doi:10.1007/s001220100701
Husz B (1941) A beteg növény és gyógyítása (The diseased plant and its healing). Királyi Magyar Természettudományi Társulat, Budapest
Khan RR, Bariana HS, Dholakia BB (2005) Molecular mapping of stem and leaf rust resistance in wheat. Theor Appl Genet 111:846–850. doi:10.1007/s00122-005-0005-4
Knott DR (1989) The wheat rusts: breeding for resistance. Springer-Verlag, Berlin/Heidelberg
Kőszegi B, Linc G, Juhász L, Láng L, Molnár-Láng M (2000) Occurrence of the 1RS/1BL wheat—rye translocation in Hungarian wheat varieties. Acta Agr Hung 48:27–236
Kuraparthy V, Sood S, Chhuneja P et al (2007) A cryptic wheat-Aegilops triuncialis translocation with leaf rust resistance gene Lr58. Crop Sci 47:1995–2003. doi:10.2135/cropsci2007.01.0038
Lagudah ES, McFadden H, Singh RP et al (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor Appl Genet 114:21–30. doi:10.1007/s00122-006-0406-z
Mago R, Miah H, Lawrence GJ et al (2005) High-resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26, and Yr9 on the short arm of rye chromosome 1. Theor Appl Genet 112:41–50. doi:10.1007/s00122-005-0098-9
Mateos-Hernandez M, Singh R, Hulbert SH et al (2006) Targeted mapping of ESTs linked to the adult plant resistance gene Lr46 in wheat using synteny with rice. Funct Integr Genomics 6:122–131. doi:10.1007/s10142-005-0017-9
McCartney CA, Somers DJ, McCallum BD et al (2005) Microsatellite tagging of the leaf rust resistance gene Lr16 on wheat chromosome 2BSc. Mol Breed 15:329–337. doi:10.1007/s11032-004-5948-7
McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts—an atlas of resistance genes. Kluwer, Dordrecht, Boston, London
Mcintosh RA, Yamazaki Y, Dubcovsky J et al. (2008) Catalogue of gene symbols for wheat. In: Komugi—Integrated wheat science database. http://www.shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp. Accessed 28 Nov 2008
Mebrate SA, Oerke EC, Dehne HW et al (2008) Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers. Euphytica 162:457–466. doi:10.1007/s10681-007-9615-z
Melchinger AE (1990) Use of molecular markers in breeding for oligogenic disease resistance. Plant Breed 104:1–19. doi:10.1111/j.1439-0523.1990.tb00396.x
Mohler V, Hsam SLK, Zeller FJ et al (2001) An STS marker distinguishing the rye-derived powdery mildew resistance alleles at the Pm8/Pm17 locus of common wheat. Plant Breed 120:448–450. doi:10.1046/j.1439-0523.2001.00622.x
Nelson RR (1978) Genetics of horizontal resistance to plant diseases. Annu Rev Phytopathol 16:359–378. doi:10.1146/annurev.py.16.090178.002043
Pink DAC (2002) Strategies using genes for non-durable disease resistance. Euphytica 124:227–236. doi:10.1023/A:1015638718242
Pink D, Puddephat I (1999) Deployment of disease resistance genes by plant transformation—a ‘mix and match’ approach. Trends Plant Sci 4:71–75. doi:10.1016/S1360-1385(98)01372-7
Prabhu KV, Gupta SK, Charpe A et al (2004) SCAR marker tagged to the alien leaf rust resistance gene Lr19 uniquely marking the Agropyron elongatum gene Lr24 in wheat: a revision. Plant Breed 123:417–420. doi:10.1111/j.1439-0523.2004.00971.x
Pretorius ZA, Singh RP, Wagoire WW, Payne TS (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Dis 84:203. doi:10.1094/PDIS.2000.84.2.203B
Prins R, Groenewald JZ, Marais GF et al (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor Appl Genet 103:618–624. doi:10.1007/PL00002918
Procunier D (2004) Disease resistance. Leaf rust resistance Lr29 - Lr25. http://maswheat.ucdavis.edu/protocols/Lr29/index.htm. Accessed 28 Nov 2008
Procunier JD, Townely-Smith TF, Fox S et al (1995) PCR-based RAPD/DGGE markers linked to leaf rust resistance genes Lr29 and Lr25 in wheat (Triticum aestivum L.). J Genet Breed 49:87–92
Qiu JW, Schurch AC, Yahiaoui N et al (2007) Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat. Theor Appl Genet 115:159–168. doi:10.1007/s00122-007-0551-z
Raupp WJ, Sukhwinder-Singh Brown-GuerdiraGL et al (2001) Cytogenetic and molecular mapping of the leaf rust resistance gene Lr39 in wheat. Theor Appl Genet 102:347–352. doi:10.1007/s001220051652
Robert O, Abelard C, Dedryver F (1999) Identification of molecular markers for the detection of the yellow rust resistance gene Yr17 in wheat. Mol Breed 5:167–175. doi:10.1023/A:1009672021411
Robert O, Dedryver F, Rolland B et al (2000) Relationships between molecular identification of the gene Yr17 and adult plant resistance against stripe and leaf rust in bread wheat varieties. Acta Phytopathol Entomol Hung 35:59–63
Rogers OS, Bendich JA (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69–76. doi:10.1007/BF00020088
Schachermayr G, Siedler H, Gale DM, Winzeler H, Winzeler M, Keller B (1994) Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor Appl Genet 88:110–115. doi:10.1007/BF00222402
Seyfarth R, Feuillet C, Schachermayr G et al (1999) Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat. Theor Appl Genet 99:554–560. doi:10.1007/s001220051268
Seyfarth R, Feuillet Schachermayr G et al (2000) Molecular mapping of the adult-plant rust resistance gene Lr13 in wheat Triticum aestivum L. J Genet Breed 54:193–198
Singh RP, Rajaram S (1992) Genetics of adult-plant resistance to leaf rust in ‘Frontana’ and three CIMMYT wheats. Genome 35:24–31
Singh RP, Rajaram S (2002) Breeding for disease resistance in wheat. In: Curtis BC, Rajaram S, Gómez Macpherson H (eds) Bread wheat improvement and production. FAO, Rome, pp 252–270
Stubbs RW, Prescott EE, Saari EE, Dubin HJ (1986) Cereal disease methodology manual. CIMMYT, Mexico
Szunics L, Lu Szunics, Balla L (1991) Termesztett lisztharmat- és rozsda rezisztens búzafajták hatása a kórokozókra (Effect of the cultivated powdery mildew and rust resistant wheat varieties on the pathogens). Novenytermeles 40:395–404
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421. doi:10.1111/j.1365-3180.1974.tb01084.x
Zhuang JJ, Jia X (1983) Increasing differentiation frequencies in wheat pollen callus. In: Hu H, Vega MR (eds) Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing, pp 431–448
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This research was funded in part by the BioExploit (FOOD-CT-2005-513959) FP6 project and by the NAP_BIO_06-PlantResource project (NKTH).
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Vida, G., Gál, M., Uhrin, A. et al. Molecular markers for the identification of resistance genes and marker-assisted selection in breeding wheat for leaf rust resistance. Euphytica 170, 67–76 (2009). https://doi.org/10.1007/s10681-009-9945-0
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DOI: https://doi.org/10.1007/s10681-009-9945-0