Skip to main content
SpringerLink
Log in

STS markers for powdery mildew resistance gene Pm6 in wheat

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The powdery mildew resistance gene Pm6, transferred to common wheat from the tetraploid Triticum timopheevii, is effective in most epidemic areas for powdery mildew in China. RFLP probe BCD135 was previously associated with Pm6. In the present research, four STS primers (NAU/STSBCD135-1, NAU/STSBCD135-2, STS003 and STS004) were designed from the sequence data of BCD135. These primers were used for PCR amplification using the genomic DNA of resistant near-isogenic lines with Pm6 and their recurrent parent, cv. Prins. No polymorphic product was observed using primers STS003 and STS004; however, primers NAU/STSBCD135-1 and NAU/STSBCD135-2 amplified two and one bands, respectively, polymorphic between the resistant near-isogenic-lines and Prins. The two primers were then used to amplify the F2 population from the cross IGV1-465 (FAO163b/7*Prins) × Prins. The amplification and the powdery mildew resistance identification data were analyzed using the software Mapmaker 3.0. The results indicated that both NAU/STSBCD135-1 and NAU/STSBCD135-2 were closely linked to Pm6 with a genetic distance of 0.8 cM. A total of 175 commercial varieties without Pm6 from different ecological areas of China were tested using marker NAU/STSBCD135-2 and none of them amplified the 230 bp-specific band. This marker thus has high practicability and can be used in MAS of Pm6 in wheat breeding programs for powdery mildew resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Bennett FGA (1984) Resistance to powdery mildew in wheat: a review of its use in agricuture and breeding programmes. Plant Pathol 33:279–300

    Article  Google Scholar 

  • Bennett FGA, van Kints T (1983) Mildew of wheat U.K. Cereal Pathogen Virulence Survey Annual Report. National Institute of Agricultural Botany, Cambridge, UK, pp 7–21

    Google Scholar 

  • Bryan GK, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Isolation and characterization of microsatellites from hexaploid bread wheat. Theor Appl Genet 94:557–563

    Article  CAS  Google Scholar 

  • Cai SB, Cheng SH, Wu JZ, Yan W (2005) Evaluation, improvement and utilization of introducted wheat reserve resource resistant to powdery mildew. Acta Tritical Crops 25:116–120

    Google Scholar 

  • Dweikat I, Zhang W, Ohm H (2002) Development of STS markers linked to Hessian fly resistance gene H6 in wheat. Theor Appl Genet 105:766–770

    Article  PubMed  CAS  Google Scholar 

  • Feuillet C, Messmer M, Schacehermayr G, Keller B (1995) Genetic and physical characterization of the Lr1 leaf rust resistance locus in wheat (Triticum aestivum L.). Mol Gen Genet 248:553–562

    Article  PubMed  CAS  Google Scholar 

  • Hsam SLK, Lapochkina IF, Zeller FJ (2003) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 8. Gene Pm32 in a wheat-Aegilops speltoides translocation line. Euphytica 133:367–370

    Article  Google Scholar 

  • Hu XY, Ohm HW, Dweikat I (1997) Identification of RAPD markers linked to the gene Pm1 for resistance to powdery mildew in wheat. Theor Appl Genet 94:832–840

    Article  CAS  Google Scholar 

  • Ji JH, CAO AZ, Wang HY, Qin B, Wang SL, Kong F, Chen PD, Liu DJ, Wang XE (2007) Discrimination of Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers. Hereditas (Beijing) (In Press) (In Chinese with English abstract)

  • Jorgensen JH, Jensen CJ (1972) Genes for resistance to wheat powdery mildew in derivatives of Triticum timopheevi and T carthlicum. Euphytica 21:121–128

    Article  Google Scholar 

  • Jorgensen JH, Jensen CJ (1973) Gene Pm6 for resistance to powdery mildew in wheat. Euphytica 22:423

    Article  Google Scholar 

  • Kameswara Rao K, Lakshminarasu M, Jena KK (2002) DNA markers and marker-assisted breeding for durable resistance to bacterial blight disease in rice. Biotechnol Adv 20:33–47

    Article  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Leath S, Heun M (1990) Identification of powdery mildew resistance genes in cultivars of soft red winter wheats. Plant Dis 74:747–752

    Article  Google Scholar 

  • Li Q, Wan JM (2005) SSRHunter. Development of a local searching software for SSR sites. Hereditas (Beijing) 27:808–810

    Google Scholar 

  • Lincoln SE, Daly MJ, Lander ES (1993) Constructing linkage maps with MAPMAKER/Exp Version 3.0. A tutorial reference manual, 3rd edn. Whitehead Institute for Medical Research, Cambridge, MA

  • Ma ZQ, Wei JB, Cheng SH (2004) PCR-based markers for the powdery mildew resistance gene Pm4a in wheat. Theor Appl Genet 109:140–145

    Article  PubMed  CAS  Google Scholar 

  • McIntosh RA, Yamazaki Y, Devos KM, Dubcovsky J, Rogers WJ, Appels R (2003) Catalogue of gene symbols for wheat. In: Pogna NE, Romano M, Pogna EA, Galterio G (eds) Proc 10th Int Wheat Genet Symp vol 4. Instituto Sperimentale per la Cerealcoltura, Rome

  • Miranda LM, Murphy JP, Marshall D, Leath S (2006) Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theor Appl Genet 113:1497–1504

    Article  PubMed  CAS  Google Scholar 

  • Naik S, Gill KS, Prakasa Rao VS, Gupta VS, Tamhankar SA, Pujar S, Gill BS, Ranjekar PK (1998) Identification of a STS marker linked to the Aegilops speltoides-derived leaf rust resistance gene Lr28 in wheat. Theor Appl Genet 97:535–540

    Article  CAS  Google Scholar 

  • Nyquist WE (1963) Inheritance of powdery mildew resistance in hybrids involving a common wheat strain derived from Triticum timopheevi. Crop Sci 3:40–43

    Article  Google Scholar 

  • Olson M, Hood L, Cantor C, Botstein D (1989) A common language for physical mapping of the human genome. Science 245:1434–1435

    Article  PubMed  CAS  Google Scholar 

  • Park YJ, Dixit A, Yoo JW, Bennetzen J (2004) Further evidence of microcollinearity between barley and rice genomes at two orthologous regions. Mol Cells 17:492–502

    PubMed  CAS  Google Scholar 

  • Parker GD, Langridge P (2000) Development of a STS marker linked to a major locus controlling flour colour in wheat (Triticum aestivum L.). Mol Breed 6:169–174

    Article  CAS  Google Scholar 

  • Prins R, Groenewald JZ, Marais GF, Snape JW, Koebner RMD (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor Appl Genet 103:618–624

    Article  CAS  Google Scholar 

  • Qi LL, Wang SL, Chen PD, Liu DJ, Friebe B, Gill BS (1997) Molecular cytogenetic analysis of Leymus racemosus chromosomes added to wheat. Theor Appl Genet 95:1084–1091

    Article  CAS  Google Scholar 

  • Rostoks N, Park YJ, Ramakrishna W, Ma J, Druka A, Shiloff BA, SanMiguel PJ, Jiang Z, Brueggeman R, Sandhu D, Gill K, Bennetzen JL, Kleinhofs A (2002) Genomic sequencing reveal gene content, genomic organization, and recombination relationships in barley. Funct Integr Genomics 2:51–59

    Article  PubMed  CAS  Google Scholar 

  • Seyfarth R, Feuillet C, Schachermayr G, Winzeler M, Keller B (1999) Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat. Theor Appl Genet 99:554–560

    Article  CAS  Google Scholar 

  • Smith PH, Koebner RMD, Boyd LA (2002) The development of a STS marker linked to a yellow rust resistance derived from the wheat cultivar Moro. Theor Appl Genet 104:1278–1282

    Article  PubMed  CAS  Google Scholar 

  • Talbert LE, Blake NK, Chee PW, Blake TK, Magyar GM (1994) Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat. Theor Appl Genet 87:789–794

    Article  CAS  Google Scholar 

  • Talbert LE, Bruckner PL, Smith LY, Sears R, Martin TJ (1996) Development of PCR markers linked to resistance to wheat streak mosaic virus in wheat. Theor Appl Genet 93:463–467

    Article  CAS  Google Scholar 

  • Tao WJ, Liu JY, Liu DJ, Chen PD (2000) Genetic mapping of the powdery mildew resistance gene Pm6 in wheat by RFLP analysis. Theor Appl Genet 100:564–568

    Article  CAS  Google Scholar 

  • Wang XY, Qi ZJ, Chen PD, Liu DJ (2000) Identification of RAPD markers tightly linked to wheat powdery mildew resistance gene Pm6. Acta Genetica Sin 27:1072–1079

    CAS  Google Scholar 

  • Weeden NF, Tierman GM, Hemmat M, Kneen BE, Lodhi MA (1992) Inheritance and reliability of RAPD markers. In: Hoisington D, McNab A (eds) Proc symp on application of RAPD technology to plant breeding (Joint Plant Breeding Symp. Ser.), CSSA, ASHS, AGA, Minneapolis, pp 31–35

    Google Scholar 

  • Zhu ZD, Zhou RH, Kong XY, Dong YC, Jia JZ (2005) Microsatellite markers linked to two genes conferring resistance to powdery mildew in common wheat introgressed from Triticum carthlicum acc. PS5 Genome 48:585–590

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The project was supported by the Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) (20020307011), the Chinese High Tech Program of China (2006AA10Z1F6, 2006AA100101) and the Program for Changjiang Scholars and Innovative Research Teams in Universities (No. 10418). We would like to thank Dr. R. McIntosh and Dr. Yiqun Weng for their critical reading of the manuscript. We are also grateful to Prof. Yilin Zhou and Prof. Xiayu Duan, Plant Protection Institute, Chinese Academy of Agricultural Sciences, for kindly supplying the Blumeria graminis isolates.

Author information

Authors and Affiliations

  1. Cytogenetics Institute and National Key Lab for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Jiangsu, 210095, P.R. China

    Jianhui Ji, Bi Qin, Haiyan Wang, Aizhong Cao, Suling Wang, Peidu Chen, Lifang Zhuang, Yu Du, Dajun Liu & Xiue Wang

Authors
  1. Jianhui Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bi Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Haiyan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aizhong Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Suling Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Peidu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lifang Zhuang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yu Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Dajun Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Xiue Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiue Wang.

Additional information

Jianhui Ji and Bi Qin contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ji, J., Qin, B., Wang, H. et al. STS markers for powdery mildew resistance gene Pm6 in wheat. Euphytica 163, 159–165 (2008). https://doi.org/10.1007/s10681-007-9578-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-007-9578-0

Keywords

Search

Navigation