Abstract
The majority of Indians consume a lot of vegetables because of their health advantages. High concentrations of polycyclic aromatic hydrocarbons (PAHs) in vegetables may be seriously harmful to consumers’ health. The method for identifying and measuring 16 USEPA polycyclic aromatic hydrocarbons (PAHs) in samples of sewage-irrigated vegetables from three industrial cities in Haryana, India, is described in this research. Ultrasonication, liquid–liquid extraction using n-hexane as a solvent, clean-up using a Florisil column, and reversed-phase high-performance liquid chromatography with a UV detector were all included in the process. The PAHs were successfully linearized (R2 > 0.99) at various doses. Results for PAH recovery ranged from 90 to about 100%. The limit of quantification was 0.002–0.580, and the limit of detection was 0.0006–0.174 µgkg−1. Data indicated that the highest mean concentrations of ∑16 PAHs were detected in Spinach (123.36 µgkg−1), in carrot (105.09 µgkg−1), and in cucumber (63.40 µgkg−1) among leafy, underground, and fruity vegetables, respectively.
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References
Aichner, B., Glaser, B., & Zech, W. (2007). Polycyclic aromatic hydrocarbonsand polychlorinated biphenyls in urban soils from Kathmandu, Nepal. Organic Geochemistry, 38, 700–715. https://doi.org/10.1016/j.orggeochem.2006.11.002
Apiratikul, R., Pongpiachan, S., & Deelaman, W. (2021). Spatial distribution, sources and quantitative human health risk assessments of polycyclic aromatic hydrocarbons in urban and suburban soils of Chile. Environmental Geochemistry and Health, 3, 2851–2870. https://doi.org/10.1007/s10653-020-00798-7
Ashraf, M. W., & Salam, A. (2012). Polycyclic aromatic hydrocarbons (PAHs) in vegetables and fruits produced in Saudi Arabia. Bulletin of Environmental Contamination and Toxicology, 88(543), 547. https://doi.org/10.1007/s00128-012-0528-8
Bahrami, S., Moore, F., & Keshavarzi, B. (2021). Evaluation, source apportionment and health risk assessment of heavy metal and polycyclic aromatic hydrocarbons in soil and vegetable of Ahvaz metropolis. Human and Ecological Risk Assessment, 27, 71–100. https://doi.org/10.1080/10807039.2019.1692300
Bishnoi, N. R., Mehta, U., Sain, U., & Pandit, G. G. (2005). PAHs in some brands of tea. Environmental Monitoring Assessment, 107, 399–405. https://doi.org/10.1007/s10661-005-3547-7
Bishnoi, N. R., Mehta, U., & Pandit, G. G. (2006). Quantification of polycyclic aromatic hydrocarbons in fruits and vegetables using high performance liquid chromatography. Indian Journal of Chemical Technology, 13, 30–35.
Bishnoi, K., Kumar, R., & Bishnoi, N. R. (2008). Biodegradation of polycyclic aromatic hydrocarbons by white rot fungi Phanerochaete chrysosporium in sterile and unsterile soil. Journal of Scientific and Industrial Research, 67, 538–542.
Camargo, M. C. R., & Toledo, M. C. F. (2003). Polycyclic aromatic hydrocarbons in Brazilian vegetables and fruits. Food Control, 14, 49–53. https://doi.org/10.1016/S0956-7135(02)00052-X
Chen, H. W. (2007). Distribution and risk assessment of polycyclic aromatic hydrocarbons in household drinking water. Bulletin of Environmental Contamination and Toxicology, 78, 201–205. https://doi.org/10.1007/s00128-007-9124-8
Chiou, C. T., Sheng, G., & Manes, M. A. (2001). Partition-limited model for the plant uptake of organic contaminants from soil and water. Environmental Science and Technology, 35, 1437–1444. https://doi.org/10.1021/es0017561
ChooChuay, C., Pongpiachan, S., Tipmanee, D., Suttinun, O., Deelaman, W., Wang, Q., Xing, L., Li, G., Han, Y., Palakun, J., & Cao, J. (2020). Impacts of PM2.5 sources on variations in particulate chemical compounds in ambient air of Bangkok Thailand. Atmospheric Pollution Research, 11, 1657–1667. https://doi.org/10.1016/j.apr.2020.06.030
Choochuay, C., Deelaman, W., Pongpiachan, S. (2023). Polycyclic aromatic hydrocarbons in Thai and Myanmar rice: Concentrations, distribution and health concerns. Nature Environment & Pollution Technology, 22, 1097-1110 https://doi.org/10.46488/NEPT.2023.v22i03.002
Chung, N. J., Cho, J. Y., Park, S. W., Park, B. J., Hwang, S. A., & Park, T. I. (2008). Polycyclic aromatic hydrocarbons in soils and crops after irrigation of wastewater discharged from domestic sewage treatment plants. Bulletin of Environmental Contamination and Toxicology, 81, 124–127. https://doi.org/10.1007/s00128-008-9398-5
Deelaman, W., Choochuay, C., Pongpiachan, S., & Han, Y. (2023). Ecological and health risks of polycyclic aromatic hydrocarbons in the sediment core of Phayao Lake Thailand. Journal of Environmental Exposure Assessment, 2, 3. https://doi.org/10.20517/jeea.2022.29
Deelaman, W., Pongpiachan, S., Tipmanee, D., Choochuay, C., Iadtem, N., Suttinun, O., Wang, Q., Xing, L., Li, G., Han, Y., Hashmi, M.Z., & Cao, J. (2020) Source identification of polycyclic aromatic hydrocarbons in terrestrial soils in Chile. Journal of South American Earth Sciences, 99 https://doi.org/10.1016/j.jsames.2020.102514
Gao, Y., & Zhu, L. (2004). Plant uptake, accumulation and translocation of phenanthrene and pyrene in soils. Chemosphere, 55, 1169–1178. https://doi.org/10.1016/j.chemosphere.2004.01.037
Gratz, S. R., Ciolino, L. A., Mohrhaus, A. S., Gamble, B. M., Gracie, J. M., Jackson, D. S., Roetting, J. P., McCauley, H. A., Heitkemper, D. T., Fricke, F. L., Krol, W. J., Arsenault, T. L., White, J. C., Flottmeyer, M. M., & Johnson, Y. S. (2011). Screening and determination of polycyclic aromatic hydrocarbons in seafoods using QuEChERS-based extraction and high-performance liquid chromatography with fluorescence detection. Journal of AOAC International, 94, 1601–1616. https://doi.org/10.5740/jaoacint.11-035
Jia, J., Bi, C., Guo, X., Wang, X., Zhou, X., & Chen, Z. (2017). Characteristics, identification, and potential risk of polycyclic aromatic hydrocarbons in road dusts and agricultural soils from industrial sites in Shanghai, China. Environmental Science and Pollution Research, 24, 605–615. https://doi.org/10.1007/s11356-016-7818-3
Jiries, A., Al-Nasir, F., Hijazin, T.J., Al-Alawi, M., Fels, L.E., Mayyas, A., Al-Dmour, R., & Al-Madanat, O.Y. (2022). Polycyclic aromatic hydrocarbons in citrus fruit irrigated with fresh water under arid conditions: Concentrations, sources, and risk assessment. Arabian Journal of Chemistry, 15. https://doi.org/10.1016/j.arabjc.2022.104027
Kalteh, S., Rastkari, N., Shamsipour, M., Alimohammadi, M., & Yunesian, M. (2020). Health risk assessment of polycyclic aromatic hydrocarbons via dietary intake of leafy vegetables. International Journal of Environmental Analytical Chemistry, 102, 1–16. https://doi.org/10.1080/03067319.2020.1817906
Khalili, F., Shariatifar, N., Dehghani, M. H., Yaghmaeian, K., Nodehi, R. N., & Yaseri, M. (2022). The analysis and probabilistic health risk assessment of PAHs in vegetables and fruits samples marketed Tehran Chemometric. Global NEST Journal, 23, 497–508. https://doi.org/10.21203/rs.3.rs-130120/v1
Khillare, P. S., Jyethi, D. S., & Sarkar, S. (2012). Health risk assessment of polycyclic aromatic hydrocarbons and heavy metals via dietary intake of vegetables grown in the vicinity of thermal power plants. Food and Chemical Toxicology, 50, 1642–1652. https://doi.org/10.1016/j.fct.2012.01.032
Kipopoulou, A. M., Manoli, E., & Samara, C. (1999). Bioconcentration of polycyclic aromatic hydrocarbons in vegetables grown in an industrial area. Environmental Pollution, 106, 369–380. https://doi.org/10.1016/s0269-7491(99)00107-4
Lee, Y.-N., Lee, S., Kim, J.-S., Patra, J. K., & Shin, H.-S. (2019). Chemical analysis techniques and investigation of polycyclic aromatic hydrocarbons in fruit, vegetables and meats and their products. Food Chemistry, 277, 156–161. https://doi.org/10.1016/j.foodchem.2018.10.114
Li, Y. T., Li, F. B., Chen, J. J., Yang, G. Y., Wan, H. F., Zhang, T. B., Zeng, X. D., & Liu, J. M. (2008). The concentrations, distribution and sources of PAHs in agricultural soils and vegetables from Shunde, Guangdong, China. Environmental Monitoring Assessment, 139, 61–76. https://doi.org/10.1007/s10661-007-9816-x
Liu, Y., Chen, L., Jianfu, Z., Qinghui, H., Zhiliang, Z., & Hongwen, G. (2008). Distribution and sources of polycyclic aromatic hydrocarbons in surface sediments of rivers and an estuary in Shanghai China. Environmental Pollution, 154(298–305), 18045759. https://doi.org/10.1016/j.envpol.2007.10.020
Mohammed, S., Obiri, S., Ansa-Asare, O. D., Dartey, G., Kuddy, R., & Appiah, S. (2019). Assessment of concentration of polycyclic aromatic hydrocarbons (PAHs) in vegetables from farms in Accra, Ghana. Environmental Monitoring Assessment, 191, 417. https://doi.org/10.1007/s10661-019-7538-5
Nasir, F., & Batarseh, M. I. (2008). Agricultural reuse of reclaimed water and uptake of organic compounds: Pilot study at Mutah University wastewater treatment plant, Jordan. Chemosphere, 72, 1203–1214. https://doi.org/10.1016/j.chemosphere.2008.01.064
Obuobie, E., Keraita, B., Danso, G., Amoah, P., Cofie, O. O., Raschid-Sally, L., & Drechsel, P. (2006). Irrigated urban vegetable production in Ghana: Characteristics, benefits and risks. (p. 150) Accra, Ghana: IWMI-RUAF-CPWF, Accra, Ghana: IWMI
Paris, A., Ledauphin, J., Poinot, P., & Gaillard, J.-L. (2018). Polycyclic aromatic hydrocarbons in fruits and vegetables: Origin, analysis, and occurrence. Environmental Pollution, 234, 96–106. https://doi.org/10.1016/j.envpol.2017.11.028
Pongpiachan, S. (2009). Application of cloud point extraction for the determination of pyrene in natural water. Southeast Asian Journal of Tropical Medicine and Public Health, 40, 392–400.
Pongpiachan, S. (2015). A preliminary study of using polycyclic aromatic hydrocarbons as chemical tracers for traceability in soybean products. Food Control, 47, 392–400. https://doi.org/10.1016/j.foodcont.2014.07.032n
Pongpiachan, S. (2022). Discrimination of the geographical origins of rice based on polycyclic aromatic hydrocarbons. Environmental Geochemistry and Health, 44, 1619–1632. https://doi.org/10.1007/s10653-021-01039-1
Pongpiachan, S., Tipmanee, D., Choochuay, C., Deelaman, W., Iadtem, N., Wang, Q., & Poshyachinda, S. (2022). Concentrations and source identification of priority polycyclic aromatic hydrocarbons in sediment cores from south and northeast Thailand. Heliyon, 8, e10953. https://doi.org/10.1016/j.heliyon.2022.e10953
Premnath, N., Mohanrasu, K., Guru, R. R. R., Dinesh, G. H., Siva, P. G., Ananthi, V., Ponnuchamy, K., Muthusamy, G., & Arun, A. (2021). A crucial review on polycyclic aromatic hydrocarbons - Environmental occurrence and strategies for microbial degradation. Chemosphere, 280, 130608. https://doi.org/10.1016/j.chemosphere.2021.130608
Reizer, E., Viskolcz, B., & Fiser, B. (2022). Formation and growth mechanism of polycyclic aromatic hydrocarbons: A mini-review. Chemosphere, 291, 132793. https://doi.org/10.1016/j.chemosphere.2021.132793
Rocher, V., Azimi, S., Moilleron, R., & Chebbo, G. (2004). Hydrocarbons and heavy metals in the different sewer deposits in the ‘Le Marais’ catchment (Paris, France): Stocks, distributions and origins. Science of the Total Environment, 323(107–122), 15081721. https://doi.org/10.1016/j.scitotenv.2003.10.010
Sahoo, B. M., Ravi Kumar, B. V. V., Banik, B. K., & Borah, P. (2020). Polyaromatic hydrocarbons (PAHs): Structures, synthesis and their biological profile. Current Organic Synthesis, 17, 625–640. https://doi.org/10.2174/1570179417666200713182441
Savinov VM et al. (2003). Polycyclic aromatic hydrocarbons (PAHs) and organochlorines (Ocs) in bottom sediments of the GubaPachenga, Barents Sea, Russia. Science of the Total Environment, 306, 39–56. https://doi.org/10.1016/S0048-9697(02)00483-7
Sharma, P., Bano, A., Singh, S. P., Sharma, S., Xia, C., Nadda, A. K., Lam, S. S., & Tong, Y. W. (2022). Engineered microbes as effective tools for the remediation of polyaromatic aromatic hydrocarbons and heavy metals. Chemosphere, 306, 135538. https://doi.org/10.1016/j.chemosphere.2022.135538
Soclo, H. H., Garrigues, P., & Ewald, M. (2000). Origin of polycyclic aromatic hydrocarbons (PAHs) in coastal marine sediments: Case studies in Cotonou (Benin) and Aquitaine (France) areas. Marine Pollution Bulletin, 40, 387–396. https://doi.org/10.1016/S0025-326X(99)00200-3
Speer, K., Horstmann, P., Steeg, E., Kuhn, T., & Montag, A. (1990). PAH analysis in vegetable samples. Zeitschrift Für Lebensmittel-Untersuchung Und Forschung, 191, 442–448.
Sun, Y., Wu, S., & Gong, G. (2019). Trends of research on polycyclic aromatic hydrocarbons in food: A 20-year perspective from 1997 to 2017. Review-Trends in Food Science and Technology, 83, 86–98. https://doi.org/10.1016/j.tifs.2018.11.015
Tao, S., Cui, Y. H., Xu, F. L., Li, B. G., Cao, J., Liu, W. X., Schmitt, G., Wang, X. J., Shen, W. R., Qing, B. P., & Sun, R. (2004). Polycyclic aromatic hydrocarbons (PAHs) in agricultural soil and vegetables from Tianjin. Science of the Total Environment, 320, 11–24. https://doi.org/10.1016/S0048-9697(03)00453-4
Tuteja, G., Rout, C., & Bishnoi, N. R. (2011). Quantification of polycyclic aromatic hydrocarbons in leafy and underground vegetables: A case study around Panipat city, Haryana, India. Journal of Environmental Science and Technology, 4, 611–620. https://doi.org/10.3923/jest.2011.611.620
Vácha, R., Čechmánková, J., & Skála, J. (2010). Polycyclic aromatic hydrocarbons in soil and selected plants. Plant, Soil and Environment. Plant, Soil and Environment, 56, 434–443. https://doi.org/10.17221/7/2010-PSE
Vousta, D., & Samara, C. (1998). Dietary intake of trace elements and polycyclic aromatic hydrocarbons via vegetables grown in an industrial Greek area. Science of the Total Environment, 218, 203–216. https://doi.org/10.1016/S0048-9697(98)00206-X
Wang, M. J., & Jones, K. C. (1994). Uptake of chlorobenzenes by carrots from spiked and sewage sludge-amended soil. Science of the Total Environment, 28, 1260–1267.
Wang, X. C., Sun, S., Ma, H. Q., & Liu, Y. (2006). Sources and distribution of aliphatic and polyaromatic hydrocarbons in sediments of Jiaozhou Bay, Qingdao, China. Marine Pollution Bulletin, 52, 129–138. https://doi.org/10.1016/j.marpolbul.2005.08.010
Wang, C., Wu, S., Zhou, S., Shi, Y., & Song, J. (2017). Characteristics and source identification of polycyclic aromatic hydrocarbons (PAHs) in urban soils: A review. Pedosphere, 27, 17–26. https://doi.org/10.1016/S1002-0160(17)60293-5
Wennrich, L., Popp, P., & Zeibig, M. (2002). Polycyclic aromatic hydrocarbon burden in fruit and vegetable species cultivated in allotments in an industrial area. International Journal of Environmental Analytical Chemistry, 82, 667–690. https://doi.org/10.1080/0306731021000075401
WHO. (1991). Evaluation of certain foods additives and contaminants. Thirty-seventh report of the joint FAO/WHO. Geneva: Expert committee on food additives. Technical Report Series, 806,1–52.
Wild, S. R., & Jones, K. C. (1992). Organic chemicals entering agricultural soils in sewage sludges: Screening for their potential to transfer to crop plants and livestock. Science of the Total Environment, 119, 85–119. https://doi.org/10.1016/0048-9697(92)90258-T
Wild, S. R., & Jones, K. C. (1994). The significance of polynuclear aromatic hydrocarbons applied to agricultural soils in sewage sludges in the U.K. Waste Management and Research, 12, 49–59.
Wretling, S., Eriksson, A., Eskhult, G. A., & Larsson, B. (2010). Polycyclic aromatic hydrocarbons (PAHs) in Swedish smoked meat and fish. Journal of Food Composition and Analysis, 23, 264–272. https://doi.org/10.1016/j.jfca.2009.10.003
Yuan, S. Y., Wei, S. H., & Chang, B. V. (2001). Biodegradation of polycyclic aromatic hydrocarbons by a mixed culture. Chemosphere, 41, 1463–1468. https://doi.org/10.1016/S0045-6535(99)00522-6
Zhang, S., Yao, H., Lu, Y., Yu, X., Wang, J., Sun, S., Liu, M., Li, D., Li, Y. F., & Zhang, D. (2017). Uptake and translocation of polycyclic aromatic hydrocarbons (PAHs) and heavy metals by maize from soil irrigated with wastewater. SCientific Reports, 7, 12165. https://doi.org/10.1038/s41598-017-12437-w
Zhong, W., & Wang, M. (2002). Some polycyclic aromatic hydrocarbons in vegetables from Northern China. Journal of Environmental Science and Health, Part A, 37, 287–296. https://doi.org/10.1081/ESE-120002588
Zohair, A. (2006). Levels of polyaromatic hydrocarbons in Egyptian vegetables and their behavior during soaking in oxidizing agent solutions. World Journal of Agricultural Sciences, 2, 90–94.
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The authors are delighted to express their gratitude and sincerest thanks the Dean of Central Instrumentation Laboratory (CIL) of Guru Jambheshwer University of Science and Technology, Hisar, of the cartography unit. The authors are also thankful to Mr. Satyawan Technician, CIL for his kind cooperation during the sample analysis.
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Kiran Bishnoi: conceptualization, methodology, data curation, analysis, visualization, validation software, writing—original draft, review and editing; Pushpa Rani: writing—review and editing; Narsi R. Bishnoi: supervision, conceptualization, writing—review and editing. All authors read and approved the final manuscript.
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Bishnoi, K., Rani, P. & Bishnoi, N.R. Polycyclic aromatic hydrocarbons in sewage-irrigated vegetables from industrial cities in Haryana, India. Environ Monit Assess 196, 337 (2024). https://doi.org/10.1007/s10661-024-12468-7
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DOI: https://doi.org/10.1007/s10661-024-12468-7