Abstract
The efficiency of pollutant removal in sewage is mainly determined by the structure and abundance of microbial flora, which is essentially represented by the type and abundance of genes and enzymes, and the reaction mechanism is the abundance and degree of reaction of metabolic pathways and metabolic modules. In order to study the mechanism of carbon, nitrogen, and phosphorus metabolism of activated sludge microflora in the A2O process at high altitudes at different temperatures, so as to improve the removal rate of pollutants and reduce the discharge concentration of pollutants. Based on the research environment of high-altitude environmental research, the author used the A2O system as the treatment process and put Illumina MiSeq high-through sequencing technology to comprehensively dissect the reaction relationship of carbon, nitrogen and phosphorus metabolic pathways, and derived the main functional genes and enzymes of carbon, nitrogen, and phosphorus metabolism. It was demonstrated that the abundance of the most metabolic modules and functional genes tended to increase and then decrease with increasing temperature. In particular, the highest abundance and activity and the best pollutant removal efficiency were observed at 20 °C. This study will provide a valuable reference for the study of carbon metabolism, nitrogen metabolism, and phosphorus metabolism of microbial flora in sewage treatment under plateau habitats.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (no. 51868069), the Bridge Structure Wind Resistance Technology Transportation Industry Key Laboratory Open Fund Project (KLWRTBMC21-01), the Postgraduate Education Innovation Project of Tibet Agriculture & Animal Husbandry University (no. YJS2021-26), the 2022 National College Students Innovative Entrepreneurship Training Program General Project (SBR-MBR Craftsmanship and Microbial Community Structure Research in Plateau Habitat), and the Innovative Experimental Project of College Students in Tibet Autonomous Region (Research on Nitrogen Removal Efficiency and Microbial Flora of Anammox Filter in Plateau Habitat).
Funding
National Natural Science Foundation of China, 51868069, Yongchen Zong, The Bridge Structure Wind Resistance Technology Transportation Industry Key Laboratory Open Fund Project, KLWRTBMC21-01, HE Qiang, the Postgraduate Education Innovation Project of Tibet Agriculture and Animal Husbandry University,YJS2021-26, HAO Kaiyue, 2022.
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HKy designed the study, interpreted the results, and was a major contributor to writing the manuscript. HQ carried out the experiments and collected the data. ZYc conducted all the work. ZLs and GMz were involved in the gene expression experiments. All authors read and approved the final manuscript.
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Kaiyue, H., Lishuai, Z., Yongchen, Z. et al. The relationship analysis and reaction mechanism of carbon, nitrogen, and phosphorus metabolism in sewage treatment in plateau habitat. Environ Monit Assess 194, 520 (2022). https://doi.org/10.1007/s10661-022-10151-3
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DOI: https://doi.org/10.1007/s10661-022-10151-3