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Polyphasic identification of two novel Colletotrichum species causing leaf spots on mangroves in Thailand

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Abstract

Mangroves play a significant part in the marine ecosystem and harbour a plethora of microorganisms vital for various ecological functions. However, they remain vulnerable to disruptive impacts caused by fungal plant pathogens. In this study, leaves with anthracnose symptoms were collected from mangrove forests in Prachuap Khiri Khan, Thailand. Three species were identified using a polyphasic approach namely two new species – Colletotrichum avicenniae from Avicennia marina, Colletotrichum lumnitzerae from Lumnitzera racemosa, and a known species Colletotrichum tropicale. Combined analysis of ITS, gapdh, chs-1, act, and tub2 sequence data coupled with Genealogical Concordance Phylogenetic Species Recognition Analysis (GCPSRA) supported the two strains as two distinct species within the gloeosporioides species complex. The three Colletotrichum species were compared and differentiated with closely related taxa. Koch’s postulates were proven, demonstrating that C. avicenniae, C. lumnitzerae, and C. tropicale cause anthracnose leaf spots in mangroves. Understanding the classification and etiology of these fungal pathogens will contribute to a more comprehensive knowledge of mangrove biology within the marine ecosystem.

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Data availability

Publicly available datasets were analysed in this study. These data can be found here: https://www.ncbi.nlm.nih.gov.

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Acknowledgements

Permission for surveys, studies, research, or academic experiments (No. 0907.4/23579) in the National Parks (book no. 02/020). We would like to thank the National Research Council of Thailand (NRCT) grant “Total fungal diversity in a given forest area with implications towards species numbers, chemical diversity and biotechnology” (grant no. N42A650547). RS Jayawardena thanks the National Science, Research, and Innovation Fund: Thailand Science Research Innovation (Basic Research Fund 2022) entitled “Biodiversity, taxonomy and phylogeny of Colletotrichum on Citrus and Mango in Northern Thailand (662A01002)”. We thank Dr. Shaun Pennycook for his valuable suggestion on nomenclature for the new taxa described herein. HD Aumentado is grateful to the Department of Science and Technology – Science Education Institute, Taguig City, Philippines. RS Jayawardena would like to thank the Eminent scholar offered by Kyun Hee University.

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Authors and Affiliations

  1. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Herbert Dustin R. Aumentado, Alireza Armand, Chayanard Phukhamsakda, Kevin D. Hyde & Ruvishika S. Jayawardena

  2. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Herbert Dustin R. Aumentado, Alireza Armand & Ruvishika S. Jayawardena

  3. Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China

    Kevin D. Hyde

  4. Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia

    E. B. Gareth Jones

  5. Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, South Korea

    Ruvishika S. Jayawardena

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  1. Herbert Dustin R. Aumentado
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Contributions

Conceptualization, RSJ; Methodology, HDA; Formal analysis, HDA, AA, and RSJ; Investigation, HDA; Resources, RSJ; Sample Collection, HDA, CP; Data curation, HDA; Writing—original draft, HDA; Writing—review & editing, RSJ, HDA, AA, CP, EBGJ, and KDH; Supervision, RSJ; Project administration, RSJ; Funding acquisition, RSJ and EBGJ. All authors have read and agreed to the published version of the manuscript.

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Correspondence to E. B. Gareth Jones or Ruvishika S. Jayawardena.

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Aumentado, H.D.R., Armand, A., Phukhamsakda, C. et al. Polyphasic identification of two novel Colletotrichum species causing leaf spots on mangroves in Thailand. Eur J Plant Pathol (2024). https://doi.org/10.1007/s10658-024-02819-y

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