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Studies on molecular variability of coat protein gene of Papaya ringspot virus-P isolates from India

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Abstract

Papaya ringspot virus (PRSV) is the key pathogen responsible for damage of the papaya plant and significant loss of fruit yield globally. Its coat protein (CP) sequences are highly variable and provide adaptability to the virus in the papaya plant. Due to the variability, the major concern facing of papaya cultivation is the breakdown of CP-transgenic PRSV resistance. In this study, 16 PRSV isolates were collected from different locations in seven districts of Uttar Pradesh, India and the variability of the CP gene of the virus was analysed. Further, it was compared with the isolates from other geographical origins. High diversity in CP sequences of our isolates was observed when compared with other Indian isolates. Phylogenetic analysis also revealed that PRSV isolates were more divergent from isolates of other countries. This study of sequence variation in the CP could be further very useful in virus management and development of broad-spectrum virus-resistant transgenic papaya.

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Acknowledgements

The authors are thankful to University Grants Commission (UGC), New Delhi, for providing financial assistance as Dr. D.S. Kothari Postdoctoral Fellowship to Rajesh Kumar to carry out this research work.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Genetics and Plant Breeding, Rajiv Gandhi South Campus (RGSC), Banaras Hindu University, Mirzapur, 231001, India

    Rajesh Kumar

  2. Amity Institute of Virology and Immunology, Amity University, Noida, 201313, India

    Ashish Srivastava

  3. Molecular Plant Virology Lab, Department of Botany, University of Lucknow, Lucknow, 226007, India

    Aparana Srivastava, Shalini Srivastava & Vivek Prasad

  4. Molecular Plant Virology, CSIR—National Botanical Research Institute, Rana Pratap Marg, Lucknow, UP, 226001, India

    Shri Krishna Raj

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  1. Rajesh Kumar
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  2. Ashish Srivastava
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  3. Aparana Srivastava
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  4. Shalini Srivastava
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Correspondence to Rajesh Kumar.

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Kumar, R., Srivastava, A., Srivastava, A. et al. Studies on molecular variability of coat protein gene of Papaya ringspot virus-P isolates from India. Eur J Plant Pathol 155, 369–376 (2019). https://doi.org/10.1007/s10658-019-01778-z

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