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Effect of temperature, relative humidity, leaf wetness and leaf age on Spilocaea oleagina conidium germination on olive leaves

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Abstract

The effects of temperature, relative humidity (RH), leaf wetness and leaf age on conidium germination were investigated for Spilocaea oleagina, the causal organism of olive leaf spot. Detached leaves of five ages (2, 4, 6, 8 and 10 weeks after emergence), six different temperatures (5, 10, 15, 20, 25 and 30°C), eight wetness periods (0, 6, 9, 12, 18, 24, 36 and 48 h), and three RH levels (60, 80 and 100%) were tested. Results showed that percentage germination decreased linearly in proportion to leaf age (P < 0.001), being 58% at 2 weeks and 35% at 10 weeks. A polynomial equation with linear term of leaf age was developed to describe the effect of leaf age on conidium germination. Temperature significantly (P < 0.001) affected frequencies of conidium germination on wet leaves held at 100% RH, with the effective range being 5 to 25°C. The percent germination was 16.1, 23.9, 38.8, 47.8 and 35.5% germination at 5, 10, 15, 20 and 25°C, respectively, after 24 h. Polynomial models adequately described the frequencies of conidium germination at these conditions over the wetness periods. The rate of germ tube elongation followed a similar trend, except that the optimum was 15°C, with final mean lengths of 175, 228, 248, 215 and 135 μm at 5, 10, 15, 20 and 25°C, respectively after 168 h. Polynomial models satisfactorily described the relationships between temperature and germ tube elongation. Formation of appressoria, when found, occurred 6 h after the first signs of germination. The percentage of germlings with appressoria increased with increasing temperature to a maximum of 43% at 15°C, with no appressoria formed at 25°C after 48 h of incubation. Increasing wetness duration caused increasing numbers of conidia to germinate at all temperatures tested (5–25°C). The minimum leaf wetness periods required for germination at 5, 10, 15, 20 and 25°C were 24, 12, 9, 9 and 12 h, respectively. At 20°C, a shorter wetness period (6 h) was sufficient if germinating conidia were then placed in 100% RH, but not at 80 or 60%. However, no conidia germinated without free water even after 48 h of incubation at 20°C and 100% RH. The models developed in this study should be validated under field conditions. They could be developed into a forecasting component of an integrated system for the control of olive leaf spot.

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Acknowledgements

We would like to thank the New Zealand Foundation for Research, Science and Technology and the New Zealand Olive Association for funding, and Peter Alspach and Dr. Chris Frampton for their assistance in the statistical analyses.

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Authors and Affiliations

  1. Bio-Protection and Ecology Division, Lincoln University, P.O. Box 84, Lincoln, Canterbury, New Zealand

    Friday O. Obanor, E. Eirian Jones & Marlene V. Jaspers

  2. HortResearch, P. O. Box 51, Lincoln, Canterbury, New Zealand

    Friday O. Obanor & Monika Walter

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  1. Friday O. Obanor
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  2. Monika Walter
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  3. E. Eirian Jones
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  4. Marlene V. Jaspers
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Correspondence to Friday O. Obanor.

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Obanor, F.O., Walter, M., Jones, E.E. et al. Effect of temperature, relative humidity, leaf wetness and leaf age on Spilocaea oleagina conidium germination on olive leaves. Eur J Plant Pathol 120, 211–222 (2008). https://doi.org/10.1007/s10658-007-9209-6

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  • DOI: https://doi.org/10.1007/s10658-007-9209-6

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