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Mixture of Pb, Zn and Cu on root permeability and radial oxygen loss in the mangrove Bruguiera gymnorrhiza

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Abstract

A short term pot trail was employed to evaluate the exposure of mixed heavy metals (Cu, Pb and Zn) on growth, radial oxygen loss (ROL) and root anatomy in Bruguiera gymnorrhiza. The possible function of BgC4H, a cytochrome P450 gene, on root lignification was also discussed. The exposures of mixed Cu, Pb and Zn directly reduce O2 leakage at root surface. The reduced ROL inhibited by heavy metals was mainly ascribed by the changes in root anatomical features, such as decreased root porosity together with increased lignification within the exodermis. BgC4H was found to be up-regulated after 0.5-day metal exposure, and remained higher transcript levels within 3-day metal exposure when compared to control roots. Besides, the inhibited photosynthesis may also result in less oxygen can be transported to the underground roots. In summary, the mangrove B. gymnorrhiza appeared to react to external mixed metal contaminants by developing a lignified and impermeable exodermis, and such a root barrier induced by mixed Cu, Pb and Zn appeared to be an adaptive response to block metal ions enters into the roots.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No. U1901211, No. 41676086, No. 41706118, No. 4143096, No. 41876126 and No. 41506127), the National Key Research and Development Plan (No. 2017FY100700), the International Partnership Program of Chinese Academy of Sciences (No. 133244KYSB20180012), the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA23050200, No. XDA13010500 and No. XDA13020503), Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (No. GML2019ZD0305), Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences (No. ISEE2019ZR02 and No. ISEE2018ZD02), Natural Science Foundation of Guangdong province (No. 2014A030313783), Science and Technology Project of Guangdong province (No. 2016A020222011), Guangdong special branch plans young talent with scientific and technological innovation (No. 2016TQ03Z985), Guangzhou Science and Technology Project (No. 20171001013).

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Cheng, H., Wang, YS., Li, CD. et al. Mixture of Pb, Zn and Cu on root permeability and radial oxygen loss in the mangrove Bruguiera gymnorrhiza. Ecotoxicology 29, 691–697 (2020). https://doi.org/10.1007/s10646-020-02234-z

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