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Screening of PAHs and DDTs in sand and acrisols soil by a rapid solid-phase microalgal bioassay

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Abstract

Previously we have demonstrated the rapid screening potential of a newly developed solid-phase microalgal bioassay with spiked sands. In this paper, we report further comparative results using both PAHs and DDTs spiked sands and field-collected acrisols soils. Toxicity responses obtained from standard higher plant tests with three species of plants (Lolium perenne, Cynodon dactylon and Brassica chinensis) were compared with those obtained from a cocktail of microalgae (Selenastrum capricornutum, Chlorococcum hypnosporum and the indigenous Chlorococcum meneghini). The 5-day seed germination/root elongation tests were not sensitive at all in contrast to the 4-day solid-phase microalgal tests and the 28-day early seedling growth tests in both spiked sands and contaminated soils. Sensitivities of microalgal tests were generally higher than the seedling growth tests in spiked sands. Concerning the assays with contaminated soil, the responses of microalgae and higher plants varied. However, the results demonstrated that microalgae could generally act as effective surrogates to screen xenobiotic compounds at toxic level to higher plants, with the local species C. meneghini especially sensitive to reveal phytotoxic effects. This promising rapid screening solution is possible to be used in accompany with standard seedling growth tests when assessing phytotoxicities of contaminated areas, especially for acrisols soil.

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Acknowledgments

The authors acknowledge the financial support from the Strategic Research Fund from the Science Faculty, HKBU and technical assistance from Mr. W.C. Li. This study was also supported by the Area of Excellence (AoE) Scheme under the University Grants Committee of the Hong Kong Special Administrative Region (CITYU/AoE/0304/02).

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Authors and Affiliations

  1. Croucher Institute for Environmental Sciences and Department of Biology, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, P.R. China

    M. K. Chung, K. C. Cheung & M. H. Wong

  2. Institute of Hydrobiology and Aquatic Ecoscience, Jinan University, Guangzhou, P.R. China

    R. Hu

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  1. M. K. Chung
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  2. R. Hu
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  3. K. C. Cheung
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  4. M. H. Wong
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Correspondence to M. H. Wong.

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Chung, M.K., Hu, R., Cheung, K.C. et al. Screening of PAHs and DDTs in sand and acrisols soil by a rapid solid-phase microalgal bioassay. Ecotoxicology 16, 429–438 (2007). https://doi.org/10.1007/s10646-007-0146-0

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  • DOI: https://doi.org/10.1007/s10646-007-0146-0

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