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Silencing of GATA6 Suppresses SW1990 Pancreatic Cancer Cell Growth In Vitro and Up-Regulates Reactive Oxygen Species

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Abstract

Background/Aims

Pancreatic cancer has the worst prognosis of any gastrointestinal cancer with a mortality rate approaching its incidence. Previous studies have indicated that GATA6 plays a key role in organ development and function, and that abnormal expression of GATA6 may induce tumorigenesis. Meanwhile, it has been reported that generation of reactive oxygen species contributes to carcinogenesis. In this study, we set out to study the role of GATA6 expression on proliferation and apoptosis of pancreatic cancer cells and the role of reactive oxygen species.

Methods

Four target miRNA sequences against GATA6 mRNA were synthesized and used to transfect SW1990 cells. Then, GATA6 expression in SW1990 cells was examined by western blot and quantative real-time polymerase chain reaction. Cell proliferation was examined by WST-8 and colony formation assay. Cell cycle progression and apoptosis were measured by flow cytometry. We also measured the generation of reactive oxygen species by immunofluorescence and flow cytometry.

Results

RNA interference against GATA6 successfully inhibited mRNA and protein expression of GATA6 in the SW1990 pancreatic cancer cell line. Silencing of GATA6 by RNA interference inhibited cell proliferation and increased apoptosis of SW1990, and enhanced the expression of reactive oxygen species.

Conclusions

These results suggest that the RNA interference approach against GATA6 may be an effective therapeutic approach for treatment of pancreatic cancer.

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Acknowledgments

The authors thank Wanling Yang (Hong Kong University, China) for his helpful discussion of the manuscript and the Lab of Target Therapy of Sun Yat-sen Memorial Hospital for the technical assistance. This research was supported in part by a grant from the Natural Science Foundation of Guangdong Province (No. 815100890100013, 04009381) and Medical Scientific Research Foundation of Guangdong Province (No. B2009066).

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Gastroenterology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, No. 107 Yanjiang West Road, Guangzhou, 510120, Guangdong, People’s Republic of China

    Wen-Bo Chen, Feng-Ting Huang, Yan-Yan Zhuang, Wen-Jie Cheng, Zhi-Qiang Gu & Shi-Neng Zhang

  2. Institute of Gastroenterology and the Sixth Affiliated Hospital, Institute of Human Virology, Key Laboratory of Tropical Disease Control (Ministry of Education), Sun Yat-sen University, No. 26 Yuancun Erheng Road, Guangzhou, 510655, Guangdong, People’s Republic of China

    Feng-Ting Huang

  3. Department of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, No. 26 Yuancun Erheng Road, Guangzhou, 510655, Guangdong, People’s Republic of China

    Jian Tang

  4. Department of Oncology and Hematology, Hainan Provincial Nongken Hospital, No. 48 Building Baishuitang Road, Haikou, 570311, Hainan, People’s Republic of China

    Xiao-Hong Zhuang

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  1. Wen-Bo Chen
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  2. Feng-Ting Huang
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Corresponding author

Correspondence to Shi-Neng Zhang.

Additional information

Wen-Bo Chen and Feng-Ting Huang contributed equally to this work.

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Chen, WB., Huang, FT., Zhuang, YY. et al. Silencing of GATA6 Suppresses SW1990 Pancreatic Cancer Cell Growth In Vitro and Up-Regulates Reactive Oxygen Species. Dig Dis Sci 58, 2518–2527 (2013). https://doi.org/10.1007/s10620-013-2752-4

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  • DOI: https://doi.org/10.1007/s10620-013-2752-4

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