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Not All Side Population Cells Contain Cancer Stem-Like Cells in Human Gastric Cancer Cell Lines

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Abstract

Introduction

Side population (SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer. Many kinds of cell lines and tissue have demonstrated presence of SP cells including different gastric cancer cell lines. However, is that true all SP cells contain cancer stem-like cells in gastric cancer cell lines?

Materials and methods

MKN-45 and BGC-823 cells labeled with Hoechst 33342 were chosen to obtain SP cells, then characterized the cancer stem-like properties of SP cells both in vitro and in vivo. Five stemness–related genes expression profiles, including OCT-4, SOX-2, NANOG, CD44 and ATP-binding cassette transporters gene ABCG-2, were tested in SP and MP cells using quantitative real-time RT-PCR. Western blot was chosen to show the difference of protein expression between SP and MP cells. When inoculated into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice, SP cells from MKN-45 showed higher tumorigenesis tendency than MP cells, but SP cells from BGC-823 showed same tumorgenesis tendency as MP cells.

Conclusion

SP cells from MKN-45 possess cancer stem cell properties and proved that they were gastric cancer stem-like cells. SP cells from BGC-823 didn’t possess cancer stem cell properties and proved that not all SP cells contain cancer stem-like cells in gastric cancer cell lines.

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Acknowledgments

The authors thank Junying Jia and Chunchun Liu (Bio-physics Institute of Chinese Academy) for the help in cell sorting. This study was supported by grants from National Natural Science Fund (81172368).

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of General Surgery, General Hospital of Chinese People’s Liberation Army (PLA), Beijing, 100853, People’s Republic of China

    Haihong Zhang, Hongqing Xi, Aizhen Cai, Qijun Xia, Xin Xin Wang, Canrong Lu, Yong Zhang, Zhou Song & Lin Chen

  2. Department of General Surgery, General Hospital of Chinese PLA Air Force, Beijing, 100142, People’s Republic of China

    Haihong Zhang

  3. Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Medical Science Chinese PLA, Beijing, 100850, People’s Republic of China

    Hua Wang, Qinfang Li & Zikuan Guo

Authors
  1. Haihong Zhang
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  2. Hongqing Xi
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  3. Aizhen Cai
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  4. Qijun Xia
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  5. Xin Xin Wang
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  6. Canrong Lu
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  7. Yong Zhang
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  8. Zhou Song
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  9. Hua Wang
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  10. Qinfang Li
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  11. Lin Chen
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  12. Zikuan Guo
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Corresponding authors

Correspondence to Lin Chen or Zikuan Guo.

Additional information

Haihong Zhang and Hongqing Xi contributed equally to this work.

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Zhang, H., Xi, H., Cai, A. et al. Not All Side Population Cells Contain Cancer Stem-Like Cells in Human Gastric Cancer Cell Lines. Dig Dis Sci 58, 132–139 (2013). https://doi.org/10.1007/s10620-012-2330-1

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  • DOI: https://doi.org/10.1007/s10620-012-2330-1

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