Skip to main content
SpringerLink
Log in

Rodent IRR-219 (IgGFcγBP) and rTFF3, Expressed Mainly in the Intestinal Mucosa, Depleted During Dextran Sulfate Sodium–Induced Colitis

  • Original Article
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

IgGFcγBP and TFF3 are related with adaptation during injury, mucosal defense, and epithelial healing. In this work, we produced the polyclonal antibodies for rat IgGFcγBP or TFF3 and assessed their tissue distributions in adult and prenatal rats, rTFF3 molecular patterns under reduced and nonreduced condition, involvement of IgGFcγBP, and TFF3 in dextran sulfate sodium (DSS)-induced colitis. Polyclonal antibodies of rat IgGFcγBP or TFF3 were produced with their synthetic polypeptide. Rat TFF3 was detected in the scraped intestinal mucosa by SDS/PAGE and Western blotting. Immunohistochemical stainings of rat IgGFcγBP or TFF3 were performed in different tissues, mainly in mucin-producing tissues, of adult rat and prenatal rat intestine. Rat IgGFcγBP and TFF3 were immunohistochemically detected in the distal colon of rat colitis model induced with 7% DSS. IgGFcγBP and TFF3 were mainly expressed in the intestinal mucosa with different distribution patterns. The scattered staining was also found in the epithelium of bile duct. There was strong expression of IgGFcγBP and TFF3 in rat embryonic intestine. There were two kinds of rTFF3 complexes existed with different molecular weights, 250 and 55 kDa, under nonreduced conditions, but shifted to 6 kDa under reduced conditions. In the DSS-induced colitis model, IgGFcγBP and TFF3 were significantly decreased in the distal colon mucosa at the onset and active phases comparing with the normal control, partially recovered at the regenerative phase. Based on these findings,

IgGFcγBP and TFF3 were widely expressed in the intestinal mucosa, depleted during DSS-induced colitis. Rat TFF3 existed mainly in two complexes with 250 and 55 kDa molecular weights. The present findings indicate they are two important goblet cell-derived components possibly related to the pathogenesis of DSS-induced colitis, a rat model of ulcerative colitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Forstner JF, Forstner GG (1994) Physiology of the gastrointestinal tract. Raven, New York, pp 77–107

    Google Scholar 

  2. Van Klinken BJW, Dekker J, Büller HA, Einerhand AWC (1995) Mucin gene structure and expression: protection vs adhesion. Am J Physiol Gastrointest Liver Physiol 269:G613–G627

    CAS  Google Scholar 

  3. Hanauer SB (2006) Inflammatory bowel disease: epidemiology, pathogenesis, and therapeutic opportunities. Inflamm Bowel Dis 12(Suppl 1):S3–9

    Article  PubMed  Google Scholar 

  4. Einerhand AW, Renes IB, Makkink MK, van der Sluis M, Buller HA, and Dekker J (2002) Role of mucins in inflammatory bowel disease: important lessons from experimental models. Eur J Gastroenterol Hepatol 14:757–765

    Article  PubMed  CAS  Google Scholar 

  5. Gum JR Jr, Hicks JW, Toribara NW, Rothe EM, Lagace RE, Kim YS (1992) The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region. J Biol Chem 267:21375–21383

    PubMed  CAS  Google Scholar 

  6. Renes IB, Boshuizen JA, van Nispen DJPM, Bulsing NP, Büller HA, Dekker J, Einerhand AWC (2002) Alterations in Muc2 biosynthesis and secretion during dextran sulfate sodium-induced colitis. Am J Physiol Gastrointest Liver Physiol 282:G382–G389

    PubMed  CAS  Google Scholar 

  7. Jacobs LR, Huber PW (1985) Regional distribution and alterations of lectin binding to colorectal mucin in mucosal biopsies from controls and subjects with inflammatory bowel diseases. J Clin Invest 75:112–118

    Article  PubMed  CAS  Google Scholar 

  8. McCormick DA, Horton LW, Mee AS (1990) Mucin depletion in inflammatory bowel disease. J Clin Pathol 43:143–146

    Article  PubMed  CAS  Google Scholar 

  9. Kobayashi K, Blaser MJ, Brown WR (1989) Identification of a unique IgG Fc binding site in human intestinal epithelium. J Immunol 143:2567–2574

    PubMed  CAS  Google Scholar 

  10. Harada N, Iijima S, Kobayashi K, Yoshida T, Brown WR, Hibi T, Oshima A, Morikawa W (1997) Human IgGFc binding protein (FcgammaBP) in colonic epithelial cells exhibits mucin-like structure. J Biol Chem 272:15232–15241

    Article  PubMed  CAS  Google Scholar 

  11. Kobayashi K, Yagasaki M, Harada N, Chichibu K, Hibi T, Yoshida T, Brown WR, Morikawa M (2001) Detection of Fcγ binding protein antigen in human sera and its relation with autoimmune diseases. Immunol Lett 79:229–235

    Article  PubMed  CAS  Google Scholar 

  12. Rubin DC, Swietlicki EA, Iordanov H, Fritsch C, Levin MS (2000) Novel goblet cell gene related to IgGFcγBP is regulated in adapting gut after small bowel resection. Am J Physiol Gastrointest Liver Physiol 279:G1003–G1010

    PubMed  CAS  Google Scholar 

  13. Suemori S, Lynch-Devaney K, Podolsky DK (1991) Identification and characterization of rat intestinal trefoil factor: tissue- and cell-specific member of the trefoil protein family. Proc Natl Acad Sci USA 88:11017–11021

    Article  PubMed  CAS  Google Scholar 

  14. Thim L, May FE (2005) Structure of mammalian trefoil factors and functional insights. Cell Mol Life Sci 62:2956–2973

    Article  PubMed  CAS  Google Scholar 

  15. Hoffmann W (2005) Trefoil factors TFF (trefoil factor family) peptide-triggered signals promoting mucosal restitution. Cell Mol Life Sci 62:2932–2938

    Article  PubMed  CAS  Google Scholar 

  16. Kalabis J, Rosenberg I, Podolsky DK (2006) Vangl1 protein acts as a downstream effector of itf/tff3 signaling and regulates wound healing of intestinal epithelium. J Biol Chem Jan 12 [Epub ahead of print]

  17. Thim L, Mortz E (2000) Isolation and characterization of putative trefoil peptide receptors. Regul Pept 90:61–68

    Article  PubMed  CAS  Google Scholar 

  18. Otto WR, Thim L (2005) Trefoil factor family-interacting proteins. Cell Mol Life Sci 62:2939–2946

    Article  PubMed  CAS  Google Scholar 

  19. Kobayashi K, Ogata H, Morikawa M, Iijima S, Harada N, Yoshida T, Brown WR, Inoue N, Hamada Y, Ishii H, Watanabe M, Hibi T (2006) Distribution and partial characterization of IgG Fc binding protein in various mucin producing cells and body fluids. Gut 51:169–176

    Article  Google Scholar 

  20. Renes IB, Verburg M, Van Nispen DJ, Taminiau JA, Buller HA, Dekker J, Einerhand AD (2002) Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression. Int J Colorectal Dis 17:317–326

    Article  PubMed  Google Scholar 

  21. Frandsen EK, Jorgensen KH, Thim L (1986) Receptor binding of pancreatic spasmolytic polypeptide (PSP) in rat intestinal mucosal cell membranes inhibits the adenylate cyclase activity. Regul Pept 16:291–297

    Article  PubMed  CAS  Google Scholar 

  22. Mashimo H, Wu DC, Podolsky DK, Fishman MC (1996) Impaired defense of intestinal mucosa in mice lacking intestinal trefoil factor. Science 274:262–265

    Article  PubMed  CAS  Google Scholar 

  23. Lacy ER, Ito S (1984) Rapid epithelial restitution of the rat gastric mucosa after ethanol injury. Lab Invest 51:573–583

    PubMed  CAS  Google Scholar 

  24. Tan XD, Hsueh W, Chang H, Wei KR, Gonzalez-Crussi F (1997) Characterization of a putative receptor for intestinal trefoil factor in rat small intestine: identification by in situ binding and ligand blotting. Biochem Biophys Res Commun 237:673–677

    Article  PubMed  CAS  Google Scholar 

  25. Lin J, Nadroo AM, Chen W, Holzman IR, Fan QX, Babyatsky MW (2003) Ontogeny and prenatal expression of trefoil factor 3/ITF in the human intestine. Early Hum Dev 71:103–109

    Article  PubMed  CAS  Google Scholar 

  26. Lin J, Holzman IR, Jiang P, Babyatsky MW (1999) Expression of intestinal trefoil factor in developing rat intestine. Biol Neonate 76:92–97

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Gastroenterology, Southwestern Hospital, Third Military Medical University, Chongqing, 400038, People’s Republic of China

    Zao-Ming Feng, Dian-Chun Fang, Wen-Sheng Chen & Rong-Quan Wang

Authors
  1. Zao-Ming Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dian-Chun Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wen-Sheng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rong-Quan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rong-Quan Wang.

Additional information

Supported by the Initiative Scientific Fund of Southwest Hospital, Third Military Medical University (200303).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Feng, ZM., Fang, DC., Chen, WS. et al. Rodent IRR-219 (IgGFcγBP) and rTFF3, Expressed Mainly in the Intestinal Mucosa, Depleted During Dextran Sulfate Sodium–Induced Colitis. Dig Dis Sci 52, 2104–2112 (2007). https://doi.org/10.1007/s10620-006-9711-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10620-006-9711-2

Keywords

Search

Navigation