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Lotus root extract inhibits skin damage through suppression of collagenase production in vitro

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Abstract

Lotus root is a traditional food ingredient used primarily in Asia and is rich in polyphenols. To determine its potential use in antiphotoaging, polyphenols were extracted from lotus root with 50% ethanol, and the activity of matrix metalloproteinase (MMP) was measured in dermal cells treated with ultraviolet A (UVA). UVA exposure increased the gene expression of IL-1α, the mRNA levels of MMP-1, and hence, the levels of MMP-1 protein in HaCaT cells, whereas cells treated with lotus polyphenol (LP) normalized these values to the control. In the presence of LP at concentrations of 1 and 10 μg/mL, both the secretion of IL-1α and protein levels of MMP-1 in human keratinocyte cells significantly reduced. Similarly, in the LabCyte EPI-MODEL24, irradiation with UVA caused an increase in mRNA expression of IL-1α and MMP-1, which was prevented by adding LP to the cells. Our results with three different skin cells accordingly showed that LP may help maintain skin health through decreased levels of MMP-1 activity via its anti-inflammatory properties.

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Data availability

The data that support the findings of this study are available from the corresponding author, AI, upon reasonable request.

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Funding

This research did not receive any specific grant from funding agencies in the commercial or not-for-profit sectors.

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Authors and Affiliations

  1. Industrial Technology Center of Saga, 114 Yaemizo, Nabesima-machi, Saga, 849-0932, Japan

    Akira Iwamoto, Yumi Tsuruta & Tsuge Keisuke

  2. Saga Foods & Cosmetics Laboratory, Division of Research and Development Promotion, Saga Prefectural Industrial Innovation Center, 114 Yaemizo, Nabesima-machi, Saga, 849-0932, Japan

    Ryoko Yamauchi, Shigeki Oogai, Yasuo Nagata & Teruyoshi Yanagita

  3. Center for Industry, University and Government Cooperation, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8535, Japan

    Yasuo Nagata

  4. Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo, Honjo-machi, Saga, 840-8502, Japan

    Teruyoshi Yanagita

Authors
  1. Akira Iwamoto
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  2. Ryoko Yamauchi
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  3. Shigeki Oogai
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  4. Yumi Tsuruta
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  5. Tsuge Keisuke
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  6. Yasuo Nagata
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  7. Teruyoshi Yanagita
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Contributions

AI conceived and designed the experiment. AI and RY equally performed the experiment and wrote the paper. SO and YT partially analyzed data. TK, YN, and TY supervised and helped with the editing of the manuscript.

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Correspondence to Akira Iwamoto.

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Iwamoto, A., Yamauchi, R., Oogai, S. et al. Lotus root extract inhibits skin damage through suppression of collagenase production in vitro. Cytotechnology 74, 309–317 (2022). https://doi.org/10.1007/s10616-022-00521-7

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  • DOI: https://doi.org/10.1007/s10616-022-00521-7

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