Abstract
Camellia nitidissima Chi (Theaceae), a rare and famous plant with golden-yellow flowers, narrowly distributes in Southwest China and North Vienam. It is now endangered and its distribution is ongoing shrinked due to deforestation and collection of seeds for horticulture. From repetitive DNA enriched libraries, we reported here the development and characterization of ten microsatellite markers for this species. The number of alleles ranged from 5 to 12. Observed and expected heterozygosities ranged from 0.4583 to 0.9474, and 0.6976 to 0.9033, respectively. These ten polymorphic microsatellite loci is the first set of microsatellite markers for C. nitidissima, and they will be used for future study of conservation genetics of this species.
This is a preview of subscription content, log in via an institution to check access.
References
Deng GY, Yang ZD, Lu TL (2000) A brief review of research on yellow camellia in china. J Guangxi Agric Biol Sci 19:119–124
Doyle JJ (1991) DNA protocols for plants—CTAB total DNA isolation. In: Hewitt GM, Johnston A (eds) Molecular techniques in taxonomy. Springer, Berlin, pp 283–293
Fu LG (1992) China plant red data book. Science Press, Beijing
Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486
Lewis PO, Zaykin D (2001) Genetic data analysis: computer program for the analysis of allelic data. Version 1.0 (d16c). Free program distributed by the authors over the internet from http://lewis.eeb.uconn.edu/lewishome/software.html
Liang SY (1993) Golden Camellias. China Forestry Press, Beijing
Lunt DH, Hutchinson WF, Carvalho GR (1999) An efficient method for PCR-based isolation of microsatellite arrays (PIMA). Mol Ecol 8:891–894. doi:10.1046/j.1365-294X.1999.00636.x
Wei X, Cao HL, Jiang YS, Ye WH, Ge XJ, Li F (2008) Population genetic structure of Camellia nitidissima (Theaceae) and conservation implications. Bot Stud (Taipei, Taiwan) 49:147–153
Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evol Int J Org Evol 38:1358–1370. doi:10.2307/2408641
Yang QH, Wei X, Zeng XL, Ye WH, Yin XJ, Wang ZM, Jiang YS (2008) Seed biology and germination ecophysiology of Camellia nitidissima. For Ecol Manag 255:113–118. doi:10.1016/j.foreco.2007.08.028
Zhang HT (1996) Diagnosis on the systematic development of Theaceae. Acta Sci Natur Univ Sunyatseni 35:77–83
Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 30560015), Natural Science Foundation of Guangxi (No. 0575115) and Project of Western Program for Fostering Personal Ability of Chinese Academy of Sciences (No. 378).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chen, ZY., Jiang, YS., Wang, ZF. et al. Development and characterization of microsatellite markers for Camellia nitidissima . Conserv Genet 11, 1163–1165 (2010). https://doi.org/10.1007/s10592-009-9908-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-009-9908-y