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Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region

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Abstract

Incarvillea mairei (H. Léveillé) Grierson (Bignoniaceae) is an endemic species to the Himalaya-Hengduan Mountains region. Here, we developed 13 microsatellite markers from I. mairei using a modified biotin-streptavidin capture method. Number of alleles per locus (NA) ranged from 3 to 7 with an average of 4.615. The observed (H O) and expected (H E) heterozygosities were from 0.050 to 0.800 and from 0.249 to 0.815, respectively. Additionally, among the 13 identified microsatellite markers, 12 of them were successfully amplified in other three congeneric species, and most of them showed polymorphic. Obtained evidences suggest that these markers provide a useful tool for further study of the population genetic structure and the breeding system in this species or/and infra-generic species.

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Acknowledgments

This study was supported by National Basic Research Program of China (973 Program, No. 2007CB411603). We would like to thank Dr. Zhen-Hua Guo for revising this manuscript. And we are grateful to Dr. Yuan Huang, Mr. Xing-Feng Zhao and Miss Yang Yang for their kindly assistance on the procedure of experiments.

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Authors and Affiliations

  1. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 650204, Kunming, Yunnan, China

    Hong-Lian Ai, Hong Wang, De-Zhu Li & Jun-Bo Yang

  2. Graduate School of the Chinese Academy of Sciences, 100049, Beijing, China

    Hong-Lian Ai

  3. School of Life Science, Nanjing University, 210093, Nanjing, China

    Hong Wang

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  1. Hong-Lian Ai
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  2. Hong Wang
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  3. De-Zhu Li
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  4. Jun-Bo Yang
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Correspondence to Hong Wang.

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Ai, HL., Wang, H., Li, DZ. et al. Isolation and characterization of 13 microsatellite loci from Incarvillea mairei (Bignoniaceae), an endemic species to the Himalaya-Hengduan mountains region. Conserv Genet 10, 1613–1615 (2009). https://doi.org/10.1007/s10592-009-9808-1

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  • DOI: https://doi.org/10.1007/s10592-009-9808-1

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