Abstract
Père David’s deer is a severely bottlenecked species but without showing inbreeding depression, making it essential to develop molecular markers to explore her genetic mechanism of population recovery. In this study, we isolated 18 novel polymorphic microsatellite loci from a dinucleotide-enriched library. This suit of markers presented 2–3 alleles for each locus and their observed and expected heterozygosities were 0.057–0.610 and 0.056–0.598, respectively. These new microsatellite loci had an average of 2.12 alleles and thus contributed to relatively low exclusion probabilities of parentage and paternity testing (0.768 and 0.921). However, when these loci were examined in combination with previous microsatellite markers, overall probabilities of parentage and paternity exclusion went up to 0.905 and 0.990, respectively, showing that these 26 microsatellite loci should be adopted together in future genetic analyses for this highly inbred species.
References
Belkhir K et al (2001) GENETIX, logiciel sous Windows™ pour la génétique des populations. Laboratoire Génome, Populations, Interactions CNRS UMR 5000, Université de Montpellier II, Montpellier, France
Cao K (1993) Selection of a suitable area for reintroduction of the Père David’s deer in China. In: Ohtaishi N, Sheng H (eds) Deer of China: biology and management, Elsevier Science Publishers B. V., Amsterdam, The Netherlands
Ding Y (2004) Chinese milu research. Jinlin Publishing House for the Science and Technology. Changchun, China
Hamilton MB, Pincus EL, Di Fiore A, Flesher RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507
He LP, Wan QH, Fang SG, Xi YM (2006) Development of novel microsatellite loci and assessment of genetic diversity in the endangered Crested Ibis, Nipponia Nippon. Conserv Genet 7:157–160
Jiao Y, Ge YF, Fang SG (2007) Eight novel microsatellite markers from the Père David’s deer (Elaphurus davidianus). Conserv Genet. doi:10.1007/s10592-007-9388-x
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655
Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Sambrook J, Fritsh EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Sternicki T, Szablewski P, Szwaczkowski T (2003) Inbreeding effects on lifetime in David’s deer (Elaphurus davidianus, Milne Edwards 1866) population. J Appl Genet 44:175–183
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Yu CQ (1996) Status of genetic diversity and conservation strategy of Père David’s deer in China. Chinese Biodiver 4:130–134
Zeng Y, Jiang ZG, Li CW (2006) Genetic variability in relocated Père David’s deer (Elaphurus davidianus) populations—implications to reintroduction program. Conserv Genet 8:1051–1059
Acknowledgements
This work was supported by a special grant from the state forestry administration of China (No. 2005–4-C04) and the Nature Science Foundation of Anhui Province (No. 070413135).
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Wu, HL., Ni, XW., Zhang, LY. et al. Eighteen novel polymorphic microsatellite loci developed from the Père David’s deer (Elaphurus davidianus). Conserv Genet 9, 1679–1682 (2008). https://doi.org/10.1007/s10592-008-9523-3
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DOI: https://doi.org/10.1007/s10592-008-9523-3