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Genetic analysis of forest species Eugenia uniflora L. through of newly developed SSR markers

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Abstract

Nine microsatellite loci for genetic analysis of three populations of the tropical tree Eugenia uniflora L. (pitanga or Brazilian cherry) from fragments of semideciduous forest were developed. We used the technique of building a (GA) n and (CA) n microsatellite-enriched library by capture with streptavidin-coated magnetic beads. We assessed the polymorphism of seven microsatellites in 84 mature trees found in three areas (Ribeirão Preto, Tambaú and São José do Rio Pardo), highly impacted by the agricultural practices, in a large region among Pardo river and Mogi-Guaçu river basins, in state of São Paulo, Brazil. All loci were polymorphic, and the number of alleles was high, ranging from 6 to 24, with a mean of 14.4. All stands showed the same high level of genetic diversity (mean H E  = 0.83) and a low genetic differentiation (mean F ST = 0.031), indicating that genetic diversity was higher within rather than among populations. Seven of the nine loci were highly variable, and sufficiently informative for E. uniflora. It was concluded that these new SSR markers can be efficiently used for gene flow studies.

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References

  • Billotte N, Lagoda PJL, Risterucci AM, Baurens FC (1999) Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54:277–288

    CAS  Google Scholar 

  • Brondani RPV, Gaiotto FA, Missiaggia AA, Kirst M, Gribel R, Grattapaglia D (2003) Microsatellite markers for Ceiba pentandra (Bombacaceae), an endangered tree species of the Amazon forest. Mol Ecol Notes 3:177–179

    Article  CAS  Google Scholar 

  • Collevatti RG, Brondani RV, Grattapaglia D (1999) Development and characterisation of microsatellite markers for genetic analysis of a Brazilian endangered tree species Caryocar brasiliense. Heredity 83:748–756

    Article  PubMed  CAS  Google Scholar 

  • Dayanandan S, Dole J, Bawa K, Kesseli R (1999) Population structure delineated with microsatellite markers in fragmented populations of a tropical tree, Carapa guianensis (Meliaceae). Mol Ecol 8:1585–1592

    Article  PubMed  Google Scholar 

  • Dillenburg LR, Waechter JL, Porto ML (1993) Species composition and structure of a sandy coastal plain forest in northern Rio Grande do Sul, Brazil. In: Seeliger U (ed) Coastal plant communities of Latin America. Academic Press, San Diego, pp 349–366

    Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Garcia F, Noyer JL, Risterucci AM, Chevallier MH (2004) Genotyping of mature trees of Entandrophragma cylindricum with microsatellites. J Hered 95:454–457

    Article  PubMed  CAS  Google Scholar 

  • Grime JP (1998) Benefits of plant diversity to ecosystems: immediate, filter and founder effects. J Ecol 86:902–910

    Article  Google Scholar 

  • Hamrick JL, Godt MJW, Murawski MDA, Loveless MD (1992) Factors influencing levels of genetic diversity in woody plant species. New Forests 6:95–124

    Article  Google Scholar 

  • Holsinger KE, Gottlieb LD (1991) Conservation of rare and endangered plants: principles and prospects. In: Falk DA, Holsinger KE (eds) Genetics and conservation of rare plants. Oxford University Press, New York, pp 95–124

    Google Scholar 

  • Lewis PO, Zaykin D (2001) GDA—genetic data analysis: computer program for the analysis of allelic data, version 1.0. Available at: http://www.hydrodictyon.eeb.uconn.edu/people/plewis/software.php. Cited, 2007 August 20

  • Martins K, Chaves LJ, Buso GSC, Kageyama PY (2006) Mating system and fine-scale spatial genetic structure of Solanum lycocarpum St.Hil. (Solanaceae) in the Brazilian Cerrado. Conserv Genet 7:957–969

    Article  Google Scholar 

  • Novick RR, Dick CW, Lemes MR, Navarro C, Caccone A, Bermingham E (2003) Genetic structure of Mesoamerican populations of big-leaf mahogany (Swietenia macrophylla) inferred from microsatellite analysis. Mol Ecol 12:2885–2893

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the www for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386. Available at: http://www.frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi (cited 05 June 2007)

  • Sanguinetti CJ, Dias EN, Simpson AJG (1994) Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17:914–921

    PubMed  CAS  Google Scholar 

  • Scarano FR (2002) Structure, function and floristic relationships of plant communities in stressful habitats marginal to the Brazilian Atlantic rain forest. Ann Bot 90:517–524

    Article  PubMed  Google Scholar 

  • White GM, Boshier DH, Powell W (1999) Genetic variation within a fragmented population of Swietenia humilis. Zucc. Mol Ecol 8:1899–1909

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was financed by a grant from Sao Paulo State Government to ALAM: FAPESP 03/04199-4/2004. We also acknowledge the support of Research Pro-rectory of Sao Paulo University (USP) for ALAM and FAEPA for MAM. This study is part of the postgraduate work of first author. Ana Lilia Alzate-Marin was supported by Researcher assistantship from FAPESP. Ronai Ferreira-Ramos was supported by a postgraduate fellowship of FAPESP. The authors would like to thank to Dr. Alexandre Sebbenn and Marcela Corbo Guidugli for the statistical support, Dr. David de Jong for the language revision, and Maria do Carmo Tomitão Canas and Ana Lucia Pimentel for the technical support in microsatellite analyses.

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Correspondence to Ana Lilia Alzate-Marin.

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Ferreira-Ramos, R., Laborda, P.R., de Oliveira Santos, M. et al. Genetic analysis of forest species Eugenia uniflora L. through of newly developed SSR markers. Conserv Genet 9, 1281–1285 (2008). https://doi.org/10.1007/s10592-007-9458-0

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  • DOI: https://doi.org/10.1007/s10592-007-9458-0

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