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Polymorphic microsatellite loci for the cardinal fish (Apogon imberbis)

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Abstract

Eight polymorphic microsatellite loci were isolated and characterized for the Cardinal fish (Apogon imberbis), a coastal-reef fish endemic to the Mediterranean Sea. Characterization of 30 Cardinal fish individuals form the western Mediterranean showed moderate to high allelic diversity ranging from 6 to 19 alleles per locus. Two loci showed significant departures from Hardy-Weinberg equilibrium presumably due to null alleles. No evidence of linkage disequilibrium was found for any locus pairwise comparasions. This microsatellite set could be useful for any basic population genetic studies of this species.

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References

  • Garnaud J (1962) Monographie de l’Apogon méditerranéen, Apogon imberbis (Linné) 1758. Bull Inst Oceanogr (Monaco) 1248:1–83

    Google Scholar 

  • Glenn TC, Cary T, Dust M, Hauswaldt S, Prince K, Clifton R, Shute I (2000) Microsatellite Isolation. http://www.uga.edu/srel/DNA_Lab/protocols.htm

  • Goudet J (1995) F_STAT (2.9.3) a program for IBM compatible PCs to calculate Weir and Cockerman’s (1984) estimators of F-statistics. J Heredity 86:485–486

    Google Scholar 

  • Hamilton MB, Pincus EL, Di Fiore A, Flesher RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507

    PubMed  CAS  Google Scholar 

  • Kuiter RH, Kozawa T (1999) Pictorial guide to fishes of the Indo-West Pacific, Apogonidae Aquatic Photographics, Australia, 59pp

    Google Scholar 

  • Kuwamura T (1983) Spawning behaviour and timing of fertilization in the mouthbreeding cardinalfish Apogon notatus. Jpn J Ichthyol 30:61–71

    Google Scholar 

  • Lahnsteiner F (2003) The spermatozoa and eggs of the cardinal fish. J Fish Biol 62:115–128

    Article  Google Scholar 

  • Mabuchi K, Okuda N, Nishida M (2006) Molecular phylogeny and stripe pattern evolution in the cardinalfish genus Apogon. Mol Phylogenet Evol 38:90–99

    Article  PubMed  CAS  Google Scholar 

  • Miller-Sims V, Atema J, Kingsford MJ, Gerlach G (2004) Characterization and isolation of DNA microsatellite primers in the cardinalfish (Apogon doederleini). Mol Ecol Notes 4:336–338

    Article  CAS  Google Scholar 

  • Okuda N (2001) The costs of reproduction to males and females of a paternal mouthbreeding cardinalfish Apogon notatus. J Fish Biol 58:776–787

    Article  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. 2nd edn Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Schneider S, Roessli D, Excoffier L (2000) ARLEQUIN: A software for population genetics data analysis Ver.2.0. Genetics and Biometry Laboratory, University of Geneva, Switzerland

    Google Scholar 

  • Smith C (1992) Filial cannibalism as a reproductive strategy in care giving teleost? Neth J Zool 42:607–613

    Article  Google Scholar 

  • Thresher RE (1984) Reproduction in Reef Fishes. T.F.H. Publications, New Jersey

    Google Scholar 

  • Tortonese E (1986) Apogonidae. In: Whitehead PJP, Bauchot ML, Hureau JC, Nielsen J, Tortonese (eds), Fishes of the north-eastern Atlantic and the Mediterranean UNESCO, Paris, p 804

    Google Scholar 

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Acknowledgements

This work was funded in part by the Mexican Council for Science and Technology CONACyT and Junta de Andalucia Ref. 2003X880_1. The authors would like to thank Diego Moreno and Chemi for their help in collecting the samples.

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Correspondence to J. A. Galarza.

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Galarza, J.A., Roques, S., Carreras-Carbonell, J. et al. Polymorphic microsatellite loci for the cardinal fish (Apogon imberbis). Conserv Genet 8, 1251–1253 (2007). https://doi.org/10.1007/s10592-006-9253-3

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  • DOI: https://doi.org/10.1007/s10592-006-9253-3

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